Flashcards in Biological Molecules Deck (42)
Monosaccharide - A single subunit of sugar
Disaccharide - 2 subunits of sugar
Polysaccharide - A polymer of sugar
Name 3 monosaccharides
Name 3 disaccharides
Name 3 polysaccharides
Which monosaccharides is sucrose formed from?
Glucose and Fructose
Which monosaccharide is maltose formed from?
Which monosaccharides is lactose formed from?
Glucose and Galactose
Features of starch
Compact and insoluble storage molecule in plants - won't affect osmosis
Made of α glucose
Amylose - chains of glucose
Amylopectin - branches
It is branched to provide free ends for condensation/hydrolysis reactions
1,4 glycosidic bonds form chains
1,6 glycosidic bonds form branches
Features of glycogen
Similar to starch
Storage molecule in animals and humans
More branches (1,6 linkages) than starch
More compact than starch
Made of α glucose
Features of cellulose
Found in the cell wall of plant cells
Made of β glucose (every other is flipped upside down)
This forms long straight chains
Chains held together with hydrogen bonds
Chains form microfibrils and layers for strength
What happens when monosaccharides or disaccharides react in a condensation reaction?
H₂O taken from ends of reactants
Glycosidic bond(s) join the reactants together
A new disaccharide or polysaccharide is formed
What happens when polysaccharides or disaccharides react in a hydrolysis reaction?
Must react with H₂O for it to work
Glycosidic bond is broken
Polysaccharide/disaccharide breaks down to form monosaccharides or disaccharides
What is the word equation for the hydrolysis of lipids?
Lipids + Water --ᴸ͟ᶦ͟ᵖ͟ᵃ͟ˢ͟ᵉ͟ -> Glycerol + Fatty Acids
Structure of fatty acids
R - COOH where R is a variable group
Describe the reaction that forms a triglyceride
Triglycerides are formed from condensation reactions between glycerol and fatty acids. The carboxylic acid (COOH) groups of three fatty acids join to the OH groups of one glycerol. At each site one molecule of H₂O and an ester bond are formed. The products of the reaction are x triglycerides and 3x water molecules, where the reaction begins with x glyercol molcules and 3x fatty acids.
What are the features of saturated fatty acids?
No carbon double bonds
Straight hydrocarbon tails
Enables tails to be tightly packed together
Tend to be solid, e.g. animal fat, lard, butter, cheese
What are the features of unsaturated fatty acids?
At least one carbon double bond
Results in kinks in the hydrocarbon tail
Fatty acids cannot be tightly packed together
Fats tend to be oils, e.g. olive oil
What is the biochemical test for lipids?
1) Add ethanol and shake
2) Decant top layer and add water
Positive result: White emulsion
Describe the structure of phospholipids
Only two fatty acids
Polar molecule (charged at either end)
What are polymers of amino acids known as?
Structure of an amino acid
H₂N -- R -- COOH
What are dipeptides and how are they formed?
Dipeptides are molecules of two amino acids.
The H₂N group of one amino acid joins to the COOH group of another amino acid.
One molecule of H₂O is produced and a peptide bond is formed between the amino acids.
The dipeptide has been formed in a condensation reaction.
Polypeptide chains are also formed in this way.
What are the levels of structure of a protein?
Primary - the specific sequence of amino acids.
Secondary - folding of the chain due to hydrogen bonds, forming α-helices and β-pleats.
Tertiary - overall folding of the chain due to disulfide bridges, ionic bonds and hydrogen bonds, to give a 3D shape.
Quaternary - when proteins are made up of more than one polypeptide chain or also have a prosthetic group (e.g. haemoglobin, antibodies).
What is the biochemical test for proteins?
Add Biuret solution to the test sample
Positive result: Lilac colour
What part of an enzyme binds to the substrate and why can it do so?
The active site binds to the substrate because it is specific and complimentary to the substrate.
Describe the lock and key and induced fit models.
Lock and key
The substrate fits into the enzyme perfectly - like a key into a lock.
When the product(s) are formed, they have a different shape so are released.
The substrate is of a similar shape to the enzyme.
Enough of the substrate can fit so the enzyme flexes and becomes complimentary.
This puts strain on the bonds in the substrate.
If the question says 'describe', what word should the answer start with?
What is an inhibitor?
A inhibitor is a molecule which binds to the enzyme and decreases the rate of reaction.
What are the differences between competitive inhibitors and non-competitive inhibitors?
Competitive inhibitors bind to the active site, competing with the substrate to decrease the number of enzyme-substrate complexes. They can be outcompeted by adding more substrate.
Non-competitive inhibitors bind to elsewhere on the enzyme, changing the shape of the active site so that it is no longer complimentary to the substrate. They not only decrease but limit the rate of reaction.
What is the equation for pH?
pH = -log₁₀(H⁺)
Rearrange the equation for pH for H⁺ ion concentration
H⁺ = 10⁻ᵖᴴ
Structure of DNA
-- Monomer of DNA
-- Phosphate group
-- Deoxyribose sugar
-- Nitrogenous base
-- Join to each other in condensation reactions
-- Phosphodiester bonds between the sugar and phosphate
-- Forms phosphate-sugar backbone
-- Two hydrogen bonds form between A and T
-- Three hydrogen bonds form between G and C
Two strands give DNA more stability
Structure of RNA
Similar to DNA
Also made of nucleotides
Ribose sugar instead of deoxyribose
Uracil instead of thymine
What methods of DNA replication were theorised?
Conservative - entire double helix conserved, entire new copy made
Semi-conservative - each strand is conserved separately and acts as a template for the formation of a new strand
Dispersive - original double helix dispersed among the two new double helices
Describe the process of DNA replication
DNA helicase breaks hydrogen bonds between bases
Free nucleotides are attracted to their complimentary bases
DNA polymerase adds the nucleotides to the strands
Nucleotides join with each other by undergoing condensation reactions
Why did many scientists doubt that DNA carried the genetic code?
The relative simplicity of DNA led many scientists to doubt that it carried the genetic code.
How was the structure of DNA discovered?
Watson and Crick worked at the University of Cambridge to theorise the structure of DNA. Using Chargaff's rule of complimentary base pairing and Rosalind Franklin's X - shaped image of DNA, they were able to model the structure of DNA.
What is ATP and what is it made of?
ATP stands for adenosine triphosphate and is formed from ribose, adenine and three phosphate groups.
It stores energy in its high energy bonds between the phosphate groups.
It is the universal currency of energy exchange in biological systems.
The ATP cycle
Start with ATP
ATP splitting releases energy used for muscle contraction, active transport and biosynthesis
Hydrolysis reaction --> ADP + P
Formation of ATP requires energy from the oxidation of glucose
Condensation reaction --> ATP
What is ATP the source of energy/phosphate for?
Movement (muscle contraction)
Activation of molecules
What are the important properties of water?
Is a metabolite in many metabolic reactions, including condensation and hydrolysis reactions
Is an important solvent in which metabolic reactions occur
Has a relatively high specific heat capacity, buffering changes in temperature
Has a relatively large latent heat of vaporisation, providing a cooling effect with little loss of water through evaporation
Has strong cohesion between water molecules; this supports columns of water in the tube-like transport cells of plants and produces surface tension where water meets air.