BIOLOGICAL SAFETY LEVEL/BIOLOGICAL AGENTS/HAZARDS Flashcards
(19 cards)
1
Q
- Those that may pose minimal threat to laboratory workers
- Those with no known potential of infecting healthy people
- i.e. Bacillus subtilis and Mycobacterium gordonae
A
BSL 1
2
Q
- Those that may pose moderate threat to laboratory workers
- Those associated with laboratory acquired infections
- Those acquired through ingestion, mucus membrane, and percutaneous exposure
- i.e. Bacillus anthracis and Yersinia pestis; Enteric pathogens such as Salmonella & Shigella; S. aureus; HIV and HBV
A
BSL 2
3
Q
- Those that may pose high risk to laboratory workers
- Those with a potential for aerosol transmission (inhalation)
- i.e. Mycobacterium, Coxiella, all agents of systemic mycoses, Rickettsiae, F. tularensis, Brucella
A
BSL 3
4
Q
- Those that may pose extreme risk to lab workers
- Can cause life threatening diseases
- Organisms encountered in research institutions (RITM, PGH)
- i.e. MERS-CoV, Ebola virus
A
BSL 4
5
Q
A device that encloses a workspace Sterilizes air with infectious material; Sterilizes air through filtration using HEPA filter .Prevents laboratory acquired infections (aerosols)
A
Biological safety cabinets
6
Q
- Least effective because it has an open front
- Negative pressure, ventilated cabinet
A
BSL CLASS1
7
Q
- Unsterilized air enters and circulates within the cabinet, & the exhaust air from the cabinet is filtered by HEPA filter
- Sterilizes air to be exhausted
A
BSL CLASS 1
8
Q
- Process non-pathogens, BSL-1, Low oncogenic virus, low toxic chemicals, carcinogens
- With 1 HEPA filter
A
BSL CLASS 1
9
Q
- Sterilizes air entering and circulating within the cabinet and the exhaust air
- Sterilizes air to be exhausted and the air that flows over infectious material
A
BSL CLASS 2
10
Q
A.k.a laminar flow
A
BSL CLASS 2
11
Q
- Process bacterial and fungal pathogens, medium risk oncogenic virus, chemicals, carcinogens
- For BSL-2 & 3 organisms
A
BSL CLASS 2
12
Q
Types of BSL CLASS II
- With fixed opening
- 70% of air is recirculated
A
Class IIA
13
Q
A
14
Q
Types of BSL CLASS II
- With variable sash opening
- Exhaust air is discharged outside the building
A
BSL CLASS IIB
15
Q
- The most effective
- The system is entirely close
- The infectious material is handled using gloves attached and sealed to the cabinet
- Can process/handle highly toxic chemicals and viral pathogens
- For BSL-4 organisms
A
CLASS III
16
Q
- These are agents that pose the greatest public health threat
- Easily transmitted and highly contagious
- Can cause high mortality rate
A
CATEGORY A
Bacillus anthracis
Francisella tularensis
17
Q
- Agents with moderate morbidity and low mortality
- Not easily transmitted as category A agents
A
Category B
Rickettsia,
Coxiella,
Brucella,
E. coli O157:H7
18
Q
- Emerging pathogens that can be engineered for mass spread in the future
A
CATEGORY C
MERS-CoV,
SARS-CoV,
MDR-Tuberculosis, H1N1
19
Q
True or False
Samples for possible bioterrorism must be processed within BSC Class-II
A
True
