Biology 14: Biotech Flashcards Preview

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Flashcards in Biology 14: Biotech Deck (16)
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1

DNA Primer

small piece of DNA that allows DNA polymerase to attach

2

Gel Electrophoresis

separates DNA fragments by size

3

Nucleic Acid Probe

identifies specific DNA seg. using radioactive/ fluorescent tags

4

Sticky Ends

unattached nucleotides at ends of DNA strands after restriction enzyme cuts

5

Plasmid

extra loop of bacterial DNA

6

Polymerase Chain Reaction (PCR)

quickly makes many copies of DNA without cells

7

Recombinant DNA

DNA blended from multiple sources

8

DNA Polymerase

Enzyme that assembles nucleotides to make more DNA

9

DNA Ligase

Enzyme that "glues" DNA fragments together

10

DNA Cloning

re-programs bacteria to make many copies of a gene we want

11

DNA Fingerprinting

uses patterns of sliced and sorted DNA to identify a person

12

Restriction Enzyme

cuts DNA at set locations based on the nucleotide sequence

13

Natural Cloning

Bacterial: asexual reproduction
Mitotic Cell Division
Identical twins

14

Artificial Cloning

gene cloning: gene spliced into a plasmid, then replicated as part of normal bacterial cell division

15

Therapeutic Cloning

produce a clone embryo in order to produce unspecialized stem cells then induce stem cells to grow into many different kinds of cells, then grow specialized tissues to study or replace diseased, damaged tissue

16

Reproductive Cloning

add DNA to egg, clone larger organisms