Bioremediation Flashcards

(35 cards)

1
Q

Whats bioremediation related to?

A

biotreatment
bioreclamation
biorestoration

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2
Q

Whats Xenobiotics?

A

Any foreign object to an organism

Don’t belong

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3
Q

Subtypes of bioremediation?

A

Biostimulation
Bioaugmentation
Intrinsic bioremediation

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4
Q

Why is bioremediation multi- faceted?

A

Deals with pollutants, organisms, and environments

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5
Q

2 categories of bioremediation?

A
  1. Insitu bioremediation Directly at site of pollution
    Intrinsic bioremediation
    Engineered bioremediation
    Obvious example: oil spill on beach, phytoremediation
  2. Ex-situ bioremediation
    Removal of contaminated material for remediation at a designed place
    Landfarms, biobeds, water treatment systems
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6
Q

Limitations of bioremediation?

A
  • Adequate microorganism concentrations/populations
  • Available electron acceptors
  • Nutrients
  • Non-toxic conditions
  • Minimum carbon sources
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7
Q

3 main mechanisms of bioremediation? Best way **

A

Anaerobic
Aerobic
Sequential**

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8
Q

Organisms break organic compounds down to feed their own growth and reproduction by providing:

A
  • carbon: structure and food

- electrons: energy

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9
Q

How does bioremediation work?

A
  • Toxic compounds are food for microbes

- Looks at reduction potentials

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10
Q

List compounds from least to most biodegradable.

A
Pesticides
Chlorinated Hydrocarbons
Alcohols, esters
Aromatic hydrocarbons
Simple hydrocarbons and petroleum fuels
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11
Q

Advantages and Disadvantages of BR?

A
  • *Advantages
  • Natural process (public accepts)
  • Can achieve complete destruction of contaminants (no “pass the problem”)
  • Can be carried out on-site
  • Can be less expensive than other hazardous clean-up
  • *Disadvantages
  • Can only bioremediate certain compounds
  • Persistence/toxicity of biodegradation products?
  • Delicate/intricate process
  • Scale-up is difficult (lab to field)
  • Longer timescale than other cleanup
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12
Q

Why isn’t it a magic bandaid?

A
  • Same thing won’t work on every site
  • Need to look into whats being left behind
  • Takes a long time
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13
Q

What is the electron receptor in aerobic BR?

A

Oxygen

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14
Q

Bacterial Enzymes in BR.

A

Monooxygenases Dehydrogenases

  • break organic bonds
  • Suseptible to climatic factors
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15
Q

Indigenous pop benefits

A

If added to env- indigenous pop will outcompete

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16
Q

Bacterial remediation examples

A

Psuedomonas, Arthorobacter, Gordonia, Geobacter, Nocardia, and actinomycetes

17
Q

Fungal enzymes in BR

A

Laccases, peroxidases, lipases, cellulases, and proteases, lignin/chitin-degrading enzymes

18
Q

What is fungal BR good in?

A
  • Fluctuating Environment

- Extreme environment-loving and huge metabolic diversity

19
Q

Cons of fungal BR?

A

-Hard to grow in lab- don’t know full diversity

20
Q

Examples of fungal BR

A

Candida (degrade formaldehyde), Gibeberella (cyanide), white rot fungi (chrysosporium) can degrade DDT, TNT, hydrocarbons, pentachlorophenol

21
Q

Why are communities important?

A
  • All activities depend on community presence

- Can promote growth of specialists

22
Q

Even if we are good at uncovering the players,…

A

… we are not as well equipped to determine the functional layers

23
Q
Whats phytoremediation? Phytostabilization?
Phytovolatilization?
Phytostimulation?
Phytotransformation/phytodegradation?
Phytoextraction?
A

Phytoremediation- Use plants to convert, remove, or sequester pollutants
(heavy metals, organic compounds)
*Effective, low-cost, environmentally friendly!!

  • Phytostabilization- increase SA of roots to clean
  • Phytovolatilization- Can get taken up by plant from soil and released in air
  • Phytostimulation- plants need microbes to help roots; support microbial growth
  • Phytotransformation/ phytodegradation- remove and transferred
  • Phytoextraction- plant sucks up and stores; primary food source is a con; animals may eat toxins; highly monitered
24
Q

Where is metal contamination from?

A

Mining activities, industry, waste disposal, agriculture, atmospheric deposition

25
Whats metal immobilization?
- Complexation (bioaccumulation, biosoprtion) – exopolysaccharide, lipoproteins - Precipitation - H2S producing bacteria, siderophores, metal reduction
26
Whats metal solubilization?
- Organic acids - Siderophores - Root exudates
27
Remediation technologies in soil and water.
**Soil** Composting – add moisture, nutrients, aeration Biopiles – ex situ aeration Bioventing – in situ aeration Landfarms – apply organic materials, irrigate **Water** Injection wells – introduce amendments to ground water Infiltration – in situ amendment Bioreactors – controlled “growth chamber” (~fermentation~) Constructed wetlands – filtration, adsorption, conversion
28
Whats a biopile?
Large-scale technology in which excavated soils are mixed with soil amendments, placed on a treatment area, and bioremediated using forced aeration (adding O2 to microbes). Contaminants are reduced to carbon dioxide and water. Optimal flow rates maximize biodegradation while minimizing volatilization of contaminants.
29
What was the biopile developed by?
Developed by the Air Force Center for Environmental Excellence
30
Whats the Constructed Wetland Treatment Systems (CWTS)?
- Used physical nature of wetland to make it do what they want - Lab-scale to pilot scale to demonstration scale to operational scale - Treatment of mining effluent water - adsorption - phytoremediation - metal reduction - Sequential treatment
31
Pesticide treatment on the prairies.
- Use biobed | - Usually harm to do bioremediation because its cold and variable weather
32
What does bioremediation refer to?
Refers to many different technologies, but all are complex and often delicate processes
33
What does BR involve?
Involves microbiology, geochemistry, engineering, hydrology
34
How to get concept approved?
Learn from nature, but must be data-driven with proof-of-concept
35
What important?
Communities- not just 1 org