Biosafety And Quality Control In A Microbiology Laboratory

Question 1

Chemical fume hood vs. Biosafety cabinet

Used for dangerous chemicals

Answer 1

Chemical fume hood

Question 2

Chemical fume hood vs. Biosafety cabinet

Used for infectious biological agents

Answer 2

Biosafety cabinet

Question 3

Chemical fume hood vs. Biosafety cabinet

Protects the user

Answer 3

Chemical fume hood

Question 4

Chemical fume hood vs. Biosafety cabinet

Protects the user, the environment, and the material

Answer 4

Biosafety cabinet

Question 5

Chemical fume hood vs. Biosafety cabinet

No HEPA filter

Answer 5

Chemical fume hood

Question 6

Chemical fume hood vs. Biosafety cabinet

Must have HEPA filter

Answer 6

Biosafety cabinet

Question 7

Chemical fume hood vs. Biosafety cabinet

Exhausts air outside the building

Answer 7

Chemical fume hood

Question 8

A device that encloses a working area to protect workers from aerosol exposure and infectious disease agents.

Answer 8

Biosafety cabinets

Question 9

the air that contains the infectious materials is sterilized, either by heat, UV light or passage through a high-efficiency particulate (HEPA) resistance filter.

Answer 9

Biosafety cabinets

Question 10

Open-fronted type of cabinet with negative pressure (ventilated cabinets)

Answer 10

Class I cabinet

Question 11

It allows room (unsterilized) air to enter the cabinet, circulate around the area and expose the material within; only the air to be exhausted is sterilized using a HEPA filter.

Answer 11

Class I cabinet

Question 12

It is used for biosafety levels 2 and 3 agents

Answer 12

Class I cabinet

Question 13

It is also known as the laminar flow BSC

Answer 13

Class II cabinet

Question 14

The most commonly used BSC in a clinical microbiology laboratory (Class IIA)

Answer 14

Class II cabinet

Question 15

Sterilizes the air using HEPA filter that flows over the infectious material and the air to be exhausted.

Answer 15

Class II cabinet

Question 16

Class II cabinet is used in BSL ____ and ____ agents

Answer 16

2 and 3

Question 17

types of Class II cabinet:

has fixed opening; 70%of the air is recirculated

Answer 17

Class II A

Question 18

types of Class II cabinet:

variable sash opening; used for chemicals, radioisotopes and carcinogens.

Answer 18

Class II B

Question 19

Provides the highest level of safety to the worker

Answer 19

CLASS III CABINET

Question 20

The air coming into and going out of the cabinet is sterilized using HEPA filter and the infectious material within is handled with rubber gloves that are attached and sealed in the cabinet.

Answer 20

CLASS III CABINET

Question 21

It is used for BSL 4 agents.

Answer 21

CLASS III CABINET

Question 22

Not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment.

Answer 22

BSL-1

Question 23

Examples of BSL-1

Answer 23

Saccharomyces cerevisiae
E. coli K-12, and
non-infectious bacteria

Question 24

Moderate potential hazard to personnel and the environment. Includes
bacteria andviruses that cause mild disease to humans, or are difficult to contract via aerosol in a lab setting.

Answer 24

BSL-2

Question 25

Examples of BSL-2

Answer 25

Hepatitis A virus, Streptococcus pyogenes, Borrelia burgdorferi (Lyme disease), Salmonella species

Question 26

Microbes there can either indigenous or exotic, and they can cause serious or potentially lethal diseasethrough respiratory transmission.

Answer 26

BSL-3

Question 27

Examples of BSL-3

Answer 27

Yersinia pestis (plague), Mycobacterium tuberculosis, SARS, rabies virus, West Nile Virus, hantaviruses

Question 28

Dangerous and exotic, posing a high risk of aerosol-transmitted infections. Infections caused by t h e s e microbes are frequently fatal and without treatment or vaccines.

Answer 28

B S L - 4

Question 29

Example of B S L - 4

Answer 29

Ebola virus Smallpox virus

Question 30

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM These are agents that pose the greatest public health threat.

Answer 30

CATEGORY A agents

Question 31

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM They are easily transmitted and highly infectious.

Answer 31

CATEGORY A agents

Question 32

Examples of CATEGORY A agents

Answer 32

smallpox, Bacillus anthracis and Francisella tularensis

Question 33

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM These are agents with moderate morbidity and low mortality.

Answer 33

CATEGORY B agents

Question 34

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM Not easily transmitted as category A agents

Answer 34

CATEGORY B agents

Question 35

Examples of CATEGORY B agents

Answer 35

Coxiella burnetti, Burkholderia pseudomallei, and Rickettsia.

Question 36

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM These are the emerging pathogens

Answer 36

CATEGORY C agents

Question 37

Examples of CATEGORY C agents

Answer 37

viruses that causes yellow fever, dengue hemorrhagic fever, influenza SARS.

Question 38

Donning of PPE

Answer 38

Hand hygiene Gown Respiratory protection Eye protection Gloves

Question 39

Doffing of PPE

Answer 39

Gloves Eye protection Gown Respiratory protection Hand hygiene

Question 40

Substances which singly or in combination have a significant threat or hazard to human health or to the environment and require special handling.

Answer 40

Hazardous wastes

Question 41

Qc monitoring: Thermometer shouldbe calibrated ______

Answer 41

Periodically

Question 42

Thermometer that differ by >10C from the reference thermometer should be

Answer 42

disposed

Question 43

Thermometers should be checked ______ for the presence of gas bubbles.

Answer 43

Daily

Question 44

The percentage of carbon dioxide must be checked

Answer 44

Daily

Question 45

The speed or revolution per minute (rpm) must be checked _____ using a ______

Answer 45

Twice a year Tachometer

Question 46

Culture Media Should be checked based on their performance and sterility., and records should be kept for at least ______

Answer 46

Two years

Question 47

Media that are not quality-controlled by the laboratory must still undergo observation for

Answer 47

Moisture Sterility Breakage, and Appearance

Question 48

Reagents should be tested _____ with both positive and negative controls

Answer 48

Daily

Question 49

Uses specific strains of microorganisms from American Type Culture Collection (ATCC)

Answer 49

Antimicrobial Susceptibility