Flashcards in biotech quizerino for flow cytometry 2 Deck (4)
What are monocytes?
1 - Types of leukocytes that originate from the bone marrow.
2 - They constitute the basic cellular components of the peripheral blood, make up 5-10%.
3 - after a short developmental stage of about 1-3 days, they circulate briefly in the blood and migrate into tissues where they assume their ultimate role as macrophages.
What are macrophages?
1 - Specific type of leukocyte that are specialized for tissue specific functions such as clearance of cellular debris, immune surveillance and the inflammatory response.
2 - Produced through differentiation from monocytes.
3 - Once differentiated, lose the ability to replicated and thus, need to be replenished.
How can monocytes be induced to differentiate into macrophages.
1 - Treat with PMA
2 - Treat with Vit D3
(treat THP-1; human monocytic cell leukemia cell line)