Block 1

Question 1

Eosin stain characteristics

Answer 1

Acidic dye (negative charge)
[Na+dye-]
Red color

Question 2

Hematoxylin stain characteristics

Answer 2

Resembles basic stain characteristics
Used with a mordant
Net positive charge [dye+Cl-]

Question 3

Acidophilia

Answer 3

Reaction of cationic ionic groups with an acidic dye

Question 4

FISH Procedure

Answer 4

Fluorescence in situ hybridization. Used to simultaneously examine distribution of gene products, of chromosomes, gene expression.

Question 5

Tissue preparation

Answer 5

Fixation-terminate cell processes using formalin
Embedding medium- washed and dehydrated with alcohol then melted paraffin
Staining- with hematoxylin and dehydrated further to stain with eosin