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ANalytical techniques > Blood Analysis > Flashcards

Blood Analysis Flashcards

1
Q

functions of blood

A

transport and homoesotasis

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2
Q

composition

A

60% plasma, 40% other components

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3
Q

normal pH

A

7.4

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4
Q

plasma

A

fluid solution with dissolved components like water, proteins, gases, etc

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5
Q

plasma proteins

A

large molecules in plasma that regulate osmotic pressure

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6
Q

blood cells

A

erythrocytes
leukocytes
thrombocytes

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7
Q

erythrocytes

A

red blood cells, produced in bone marrow, non-nucleated, biconcave

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8
Q

main function of RBC

A

carry oxygen as oxyhaemaglobin (haemoglobin-protein)

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9
Q

reticulocyte

A

immatures reb blood cells with nucleus

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10
Q

leukocytes

A

white bloode cells

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11
Q

type of WBC

A

granulocytes and agranulocytes

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12
Q

granulocytes

A

70%, granular cytoplasm, multilobed

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13
Q

types of granulocytes

A

neutrophiles
eosinophiles
basophiles

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14
Q

neutrophiles

A

phagocytes in response to infections do not stain any colour, 90%

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15
Q

eosinophiles

A

stain red, they act in immflamatory responses and in allergic reaction

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16
Q

basophiles

A

stain blue, they produce histamine and heparin

17
Q

agranulocytes

A

they have a clear cytoplasm with one big nucleus

18
Q

type of agranulocytes

A

monocytes and lumphocytes

19
Q

monocytes

A

phagocytes with big nucleus as horse show

20
Q

lymphocytes

A

90%, they form memory cells and anti bodies, clear cytoplasm with big rounded nucleus

21
Q

thrombocytes

A

plateletes, involved in process of blodd clotting and release serotonin

22
Q

haematology

A

aanalysis of blood

- changes in number or appeareance to analyse probable diagnostics

23
Q

blood smapling

A

venepuncture in cephalic or jugular veins (avoid haemolysis)

24
Q

blood tubes haematology

A

EDTA (pink/red)=plasma

25
Q

blood tubes biochemistry

A

plain=serum
serum separator tube (brown)= serum
heparin (orange)= plasma

26
Q

manual PCV

A

collect sample in EDTA tube
mix gently
capillary tube en into blood (45 angle, 3/4 full)
horizontal with finger to remove capillary tube
soft clay at the end of blood
centrifuge with blood on the outside

27
Q

how many rom for PCV

A

10000 x 5min

28
Q

PCV analysis

A

low pcv means anemia

high pcv means dehydration

29
Q

stains diff-quick

A

slide dipped into fixative solution (light blue) x 5
dipped into stain solution 1 (red) x 5
dipped into stai solution 2 (puple) x 5
distilled water

30
Q

normal pcv cat

A

30-45%

31
Q

pcv normal dog

A

35-55%

ANalytical techniques (12 decks)

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