Blood Collection Equipment

Question 1

What equipment do you need for a venipuncture?

Answer 1

1. Needles
2. Tube holder
3. Tourniquet
4. Antiseptics (alcohol, chlrohexidine gluconate)
5. Vacuum collection tubes
6. Labels
7. Gloves
8. Requisition
9. Patient
10. Band aid / gauze
11. Hand sanitizer
12. Black sharpie
13. Biohazard bag
14. Gauze

Question 2

What antiseptic will you use if a patient is allergic to alcohol?

Answer 2

Chlorhexidine gluconate

Question 3

Tube order of draw

Answer 3

Yellow (SPS)
Light blue
Red
Gold
Light Green
Green
Lavender
Pink
Tan
Royal Blue
Gray
Yellow ADC

Question 4

What does SST stand for?

Answer 4

Serum Separator Tube

Question 5

What color is the SST tube?

Answer 5

Gold

Question 6

What does SPS stand for?

Answer 6

Sodium polyanethol sulfonate

Question 7

What color is the SPS tube?

Answer 7

Yellow

Question 8

What color is the ACD tube?

Answer 8

Yellow

Question 9

FUO

Answer 9

Fever with unknown origin

Question 10

What tube is used to determine the cause of an FUO?

Answer 10

Yellow SPS / Culture vial

Question 11

What is EDTA?

Answer 11

An additive that causes the blood to remain fluid for years.

Question 12

Define phagocytosis

Answer 12

when a cell eats another cell. antibiotics cause white blood cells to eat bacteria or infested cells.

Question 13

Define antiseptics

Answer 13

chemicals used to inhibit the growth and development of microorganisms, but this doesn’t kill them

Question 14

Define disinfectant

Answer 14

chemical compounds used to remove or kill pathogenic microorganisms

Question 15

essential antiseptics for blood collection

Answer 15

70% isopropyl alcohol | iodine swab | chlorhexidine gluconate

Question 16

How long does it take antiseptics to dry?

Answer 16

30-60 seconds

Question 17

Why do we use disinfectants before a venipuncture?

Answer 17

Used to prevent contamination on the skin

Question 18

Anticoagulant

Answer 18

additive that prevents clotting

ex. EDTA

Question 19

Clot activators

Answer 19

additives that promote clotting

ex.

Question 20

An anticoagulant medication

Answer 20

aspirin

Question 21

A CBC test requires whole blood collected in what color tube

Answer 21

lavender

Question 22

This color tube is used to test for Lead

Answer 22

tan

Question 23

This tube is known as serum separation tube (SST)

Answer 23

gold

Question 24

This tube has the additive EDTA in it

Answer 24

lavender

Question 25

This tube is used for STAT testing

Answer 25

orange

Question 26

This tube is used to test for nutrient determination

Answer 26

dark blue

Question 27

This needle is used for hand draws

Answer 27

butterfly needle

Question 28

How many times should a lavender top be inverted

Answer 28

8-10 times

Question 29

This tube colors is used to test for PT/INR

Answer 29

light blue

Question 30

This color tube is used to test for paternity

Answer 30

yellow acd

Question 31

The color coding for needles indicates the

Answer 31

gauge size

Question 32

This color tube is used to test a person's Blood Type

Answer 32

pink

Question 33

The amount of time a tourniquet can be applied on the arm

Answer 33

60 seconds

Question 34

This tube has no additives in it

Answer 34

red

Question 35

These tubes have the additive heparin in it

Answer 35

green

Question 36

This tube is used to test glucose tolerance

Answer 36

gray

Question 37

This part of the needle always needs to be up

Answer 37

bevel

Question 38

The hole of a needle is also known as the

Answer 38

lumen

Question 39

This holds the tube while it fills with blood

Answer 39

tube holder

Question 40

The device blood flows into as it's being collected

Answer 40

vacuum holder

Question 41

Which tube is used to test for Septicemia or FUO

Answer 41

yellow SPS

Question 42

This color tube is used to test for Hemoglobin A1C

Answer 42

lavender

Question 43

This is the liquid portion of blood

Answer 43

plasma

Question 44

Inversion times for the light blue tube

Answer 44

4 - 5

Question 45

How is serum collected?

Answer 45

centrifuge a serum tube > suction out serum from the top > transfer serum into another container for transport to the lab

Question 46

Anticoagulant tubes

Answer 46

tubes where the blood remains fluid - light blue - lavender - green (heparin) - gray - SPS

Question 47

Clot activator tubes

Answer 47

tubes where the blood clots and becomes solid - red - SST - orange (RST) - gold - marble

Question 48

Whole blood

Answer 48

blood collected in anticoagulant tubes and only requires inversion

Question 49

Plasma

Answer 49

liquid portion of the blood - collected in anticoagulant tubes and centrifuged

Question 50

Serum

Answer 50

collected in coagulant tubes and separated from blood cells via centrifuge

Question 51

Whole blood tubes

Answer 51

- yellow, SPS - lavender - yellow, ACD - pink

Question 52

Plasma tubes

Answer 52

- light blue - gray - green - light green - royal blue - tan - pink

Question 53

Serum tubes

Answer 53

- red (60 mins) - gold (30 mins) - tiger top (30 mins) - gray - light green - orange (5 mins) - royal blue

Question 54

Largest gauge of a needle

Answer 54

22

Question 55

Typical needle gauge to use on adults

Answer 55

21G

Question 56

the smaller the gauge,

Answer 56

the larger the needle

Question 57

Needles color code by

Answer 57

gauge size

Question 58

Hemolysis

Answer 58

rupture or lysis of blood cells, which can be cause by using a needle that is too small

Question 59

Gauge used for blood donations

Answer 59

18 G

Question 60

Tests done on Light blue tube

Answer 60

- coagulation tests

Question 61

Tests done on Red tubes

Answer 61

- therapeutic drug monitoring

Question 62

Tests done on Gold tubes

Answer 62

-HCG - Lytes - LFTS - Iron - Hormones - Any panels

Question 63

Tests done on Green tubes

Answer 63

- Lactate - Ammonia - Plasma K

Question 64

Tests done on Lavender tubes

Answer 64

- Blood count (CBC) - Hemaglobin A1C - Sickle cell - Platelet count - Bone marrow - Retics - ESR - CD4

Question 65

Tests done on Pearlescent tubes

Answer 65

You don't, it's a discard tube

Question 66

Tests done on Pink tubes

Answer 66

- Cross match blood donations - Transfusion medicine

Question 67

Tests done on Yellow SPS tubes

Answer 67

- blood cultures - FUO

Question 68

Tests done on Orange tubes

Answer 68

- STAT serum determinations

Question 69

Tests done on Royal blue tubes

Answer 69

- nutritional tracing - toxicology tests (overdosing) - Lead - Zinc - Chromium - Cobalt

Question 70

Tests done on Gray tubes

Answer 70

- pregnancy glucose - glucose testing - ETOH (alcohol) - Lactose testing

Question 71

Tests done on Yellow ACD

Answer 71

- paternity testing - organ donation compatibility

Question 72

Which anticoagulant is found in the white top tube?

Answer 72

EDTA

Question 73

For capillary collection from newborns, a lancet of which lengths should be used to avoid penetrating the bone?

Answer 73

anything less than 2.0 mm

Question 74

If a heparin tube is underfilled, which of the following analytes performed with blood from a heparin tube will have false values?

Answer 74

Troponin

Question 75

What is an anticoagulant used in blood donations?

Answer 75

ACD

Question 76

The glycolytic inhibitor is found in which tube

Answer 76

gray

Question 77

Which tube cannot be used to collect for the CPK, Troponin T, and other cardiac assays since the additive would destroy the analytes?

Answer 77

gray

Question 78

Which tube should be used for collection of blood for the complete blood count?

Answer 78

lavender

Question 79

Which tube should be used for collecting the blood to go to the blood bank for the type and cross-match?

Answer 79

pink

Question 80

D-Dimer

Answer 80

Light blue

Question 81

Panels

Answer 81

SST

Question 82

Bilirubin

Answer 82

SST

Question 83

BUN

Answer 83

SST

Question 84

Calcium

Answer 84

SST

Question 85

Cholesterol

Answer 85

SST

Question 86

Creatinine

Answer 86

SST

Question 87

Ferritin

Answer 87

SST

Question 88

Follicle stimulating hormone

Answer 88

SST

Question 89

Glucose, Fasting

Answer 89

SST

Question 90

HCG

Answer 90

SST

Question 91

HIV

Answer 91

SST

Question 92

H-Pylori

Answer 92

SST

Question 93

Lyme Disease

Answer 93

SST

Question 94

Magnesium

Answer 94

SST

Question 95

Potassium

Answer 95

SST

Question 96

Prostate Specific Antigen

Answer 96

SST

Question 97

Sodium

Answer 97

SST

Question 98

Testosterone

Answer 98

SST

Question 99

Vitamin B12

Answer 99

SST

Question 100

Thyroid Stimulating Hormone

Answer 100

SST

Question 101

Antibody identification

Answer 101

Red

Question 102

Antibody screening

Answer 102

Red

Question 103

Digoxin

Answer 103

Red

Question 104

Lithium

Answer 104

Red

Question 105

Serum tests

Answer 105

Red

Question 106

Discard tube

Answer 106

Red

Question 107

Blood bank type

Answer 107

Lavender

Question 108

Bone marrow

Answer 108

Lavender

Question 109

Cerebrospinal fluid

Answer 109

Lavender

Question 110

Complete blood count (CBC)

Answer 110

Lavender

Question 111

Platelets

Answer 111

Lavender

Question 112

Hematocrit

Answer 112

Lavender

Question 113

Hemoglobin

Answer 113

Lavender

Question 114

Erythrocyte sedimentation rate

Answer 114

Lavender

Question 115

Hemoglobin A1C

Answer 115

Lavender

Question 116

White blood cell

Answer 116

Lavender

Question 117

Sickle cell

Answer 117

Lavender

Question 118

Red blood cells

Answer 118

Lavender

Question 119

Glucose tolerance

Answer 119

Gray

Question 120

Lactose tolerance

Answer 120

Gray

Question 121

Pregnancy glucose testing

Answer 121

Gray