Blood Component Preparation and Transfusion Therapy Flashcards by Ramon Angelo Fajarito

Blood collected from a donor before separation into its components

Whole Blood

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Parts of whole blood that can be separated by centrifugation consists of

RBC
Plasma
Cryoprecipitated Antihemophilic Factor
Platelets

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Treatment of a disease or condition by the use of blood or blood derivatives

Hemotheraphy

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Collection of blood in a sterile blood container

Closed System

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Collection or exposure to air through an open port that would shorten the expiration because of potential bacterial contamination

Open System

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What solution works to prevent clotting and extend the sstorage of Red Blood Cells

Anticoagulant Preservative Solution

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The volume of anticoagulant preservative solution in the primary collection bag is ___mL or _____ mL

63 mL or 70 mL

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The standard whole blood collection volume is _____ mL for blood collected in a bag containing ____ mL of anticoagulant or ______ mL for the larger volume bag containing _____ mL of anticoagulant preservative

450+-45
63mL
500+_50
70 mL

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Reducing the anticoagulant may be necessary if a unit of blood is collected from an individual weighing less than ____lb

110 lb

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Heavy Spin for PRBC, Platelet concentrate

5000 g for 5 mintues

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Heavy Spin for Cryo, Cell free plasma

5000 G FOR 7 minutes

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Heavy Spin for platelet - rich plasma

2000 g for 3 minutes

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Binds calcium, prevents coagulation by chelating calcium, alsoprotects red cell membrane

Citrate

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Provides energy to RBC, supports ATP Generation by glycolytic pathway

Dextrose

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To lower the pH, Prevents caramelization

Citric Acid

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Increase ATP, prevents excessive decrease in pH

Phosphate Buffer

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Improves survival of RBC, Acts as substrate for red cell ATP synthesis

Adenine

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In Mannitol, ____ acts as membrane stabilizer

Osmotic Diuretic

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Days forCPD

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Days for CP2D

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Days for CPDA 1

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Days for AS-1 (Adsol)

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Days for AS-3 (Optisol)

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It is used in some blood centers to regenerate ATP and 2,3 DGP

Rejuvination Solutions

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In rejuvenation solution, red cells stored in the liquid state for fewer than ___ days after their outdate are rejuvanted for ___ to ___ hours, at ___ deg C with the solution

3 DAYS 1 - 4HOURS 37 Deg C

What is PIPA in rejuvination solution

Phosphate, Inosine, Pyruvate, Adenine

Only FDA approved rejuvenation solution in US

Rejuvesol

What is PIGPA in rejuvination solution

Phosphate, Inosine, Gluose, Pyruvate, Adenine

Whole blood is the unmodified component drawn from a donor, which consists of ___, ___, ___, and ____ with anticoagulant preservative solution

Erythrocyte, Leukocyte, and Plasma Proteins

Wholeb lood is stored in a monitored refrigerator at ___ - ____ Deg C for ___ days if collected in CPD or for ___ days if collected in CPDA 1

1-6 Deg C 21 days 35 days

Storage for WHOLE BLOOD

1-6C

Transport for WHOLE BLOOD

1-10 C

Heparin for WHOLE BLOOD

2 days

RBC contains ___, which transports oxygen from tissues and the bloodstream

Hemoglobin

Transfusing __ unit of RBCs usually increases the hemoglobin by ___ g/dL and increases the hematocrit by ___ %

1 unit 1 g/dL 35 %

Storage of RBC

1-6 Deg C

Shelf life of RBC

Closed (Depends on anticoagulant) Open (24 Hrs)

Is designed to remove leukocyte from whole blood without removing platelets

Inline Filters

In frozen red blood cells, freezing extends storage up to ___ years from collection when stored at or below ___ deg C

10 Years -65 deg C

Freezing can also be accomplished with a lower glycerol concentration if a _____ is used

Liquid Nitrogen Freezer

Glycerol concentration is approximately ___% and the initial freezing temperature is ___ Deg C

20% -196 Deg C

In frozen red blood cells, the maximum temperature of freezing is ____ Deg C for ____ Years

-120 Deg C 10 Years

What is the process of thawing, and removing the glycerol from Frozen RBCs

Deglycerolization

The process of glycerolization and deglycerolization involves entering of blood unit, the system is considered ____ and the prduct must be transfused within ____ days if closed system

Open System 14 Days

In Washed Red Blood Cells, the reaction can be ____, ____, or ____.

Allergic Febrile Anaphylactic

Washing is accomplished with approximately _____ mL of ____% Saline using the ________

1000 mL 0.9% SALINE Automatic blood cell processor

In Red Blood Cells Irradiated, Irradiation can be performed with a

Gamma Radiator Linear Accelerators Ultraviolet A Irradiation Nonradioisotopes equipment (xray)

The required dose of irradiation is _____ cGy or ____ Gy, the lowest dose should be _____ cGy

2500 cGy or 25 Gy 1500 cGy

The expiration date of irradiated RBCs is changed to ____ days after irradiation if the available shelf life exceeds ___ days

28 days 28 days

Transfused platelets normally circulate with a lifespan of ____ days

3-4 days

Platelet concentrates should contain at least _____ platelets per unit, and under optimal conditions, it should elevate the platelet count by about ____%L in a recipient weighing ____kg

5.5x1010 5000%L 75 kg

In pooled platelets, the approximate dose is ____ unit per ___ of body weight

1 unit per 10/kg of patient body weight

The pooled platelets should be stored at ____ to ____ deg C

20-24 deg C

It is an effective method of harvesting therapeutic dose of platelets from one individual donor

Apheresis Platelets

Plateletpheresis donors may donate as often as ____ a week or ____ ayear with an interval of ____ hours between procedures

2x a week 24 times a year 48 hours

A unit of platelets prepared by apheresis should contain a minimum of _____ platelets in _____% of the samples units

3x1011 platelets 90%

FFP (Fresh Frozen Plasma) contains all coagulation factor including ____, _____ which do not store well at temperatures greater than _____ deg C

Labile factors V VIII -18 deg C

Units of FFP and PF24 are thawed before administration in a ______ to _____ deg C waterbath for approximately _____ to ____ minutes

30-37 deg C 30-45 minutes

Standard microwave ovens should never be used because they denature ______

Plasma Proteins

FFP (Fresh Frozen Plasma) is stored at ____ to ____ deg C and should be transfused within ____ hours from thawing

1-6 deg C 24 hours

In FFP, if not transfused within 24 hours, the product should be relabeled as ______ and stored at ____ to ____ deg C for up to ____ days after thawing

"Thawed Plasma" 1-6 deg C 5 days

Storage for FFP

-18 deg C or colder

Shelf life for FFP

1 year/12 months

What is the cold insoluble precipitate that forms when a unit of FFP is thawed between 1 deg c and 6 deg C

Cryoprecipitated AHF (ANTIHEMOPHILIC FACTOR)

Factors in cryoprecipitated AHF

Von Willebrand Factor Fibrinogen (Factor 1) Factor VII Fibronectin Factor X11

Storage for Cryoprecipitated AHF

-18 deg C or colder

Shelf life for Cryoprecipitated AHF

1 year / 12 months

The primary clinical uses of CRYO are as a supplement for patients with deficiencies of ____ and _____

Factor XIII and Fibrinogen (Factor I)

In Plasma Cryoprecipitated Reduced, the product is relabeled as ______ and is stored at ____ deg C or lower for ___ year from the date of collection

Plasma Cryoprecipitated Reduced -18deg C 1 year

The CRYO Poor plasma (CPP) is used primarily in the treatment of ________ because it contains ______

Thrombotic Thrombocytopenic Purpura (TTP) ADAMTS 13

In cryoprecipitated antihemophilic factor (pooled), the frozen units first must be thawed in a ____ to ____ water bath for up to ____ minutes using ____ to prevent contamination of the ports

30-37 deg C 15 minutes over wraps

In apheresis granulocytes, the number of granulocytes in each product equals or is greater than ______

1.0x1010

Granulocytes deteriorate rapidly on storage and should be administered within ___ hours of collection.

24 hours

Storage for Apheresis Granulocyte

20-24 deg C without agitation

Shelf life for Apheresis Granulocyte

24 Hours

Shelf life for Modified Whole Blood

21 days at 1-6 deg C

Transfused platelets normally circulatewith a lifespan of how many days?

3-4 days

PLATELETS LEUOKOCYTES REDUCED Platelets collected by apheresis must have less than ____ leukocytes in ____% of the units tested

5 x 106 95%

The primary clinical use of CRYO are as a supplement for patients with deficiencies of factor ___ and ___

Factor XII Factor I

What is the protein that is reduced in Thrombotic Thrombocytopenic Purpura?

ADAMTS 13

Pooled CEYO must be administered within ___ hours of first entry and should be stored at ___ until transfusion?

4 hours Room Temperature

STORAGE for Apheresis Granulocyte

20-24 C with agitation

STORAGE for fresh frozen plasma

-18 or -65C

STORAGE for Thawed Plasma

1-6 C

Storage for Apheresis FFP

-18C

STORAGE for cryoprecipitated AHF (After Thawing)

20-24 C

For cancer patients(chemotheraphy) and recipients of hematopoietic progenitor transplants

Platelets