Compatibility Testing

Question 1

Term often considered synonymous with crossmatching

Answer 1

Compatibility Testing

Question 2

Recipient identification, sample collection and handling, and required pretransfusion testing.

Answer 2

Compatibility Testing

Question 3

Discovery of the circulation of blood by William Harvey

(YEAR)

Answer 3

1628

Question 4

Discovered ABO, early recipients often died because no understanding existed of ABO blood group system antibodies o of other blood group system alloantibodies.

(YEAR)

Answer 4

1900

Question 5

A crossmatch procedure was first attempted year ______ in ______ by _______

Answer 5

1907
New York
Weil & Ottenberg

Question 6

Antibody Screening

(YEAR)

Answer 6

1950

OR

1950 to mid 1960s

Question 7

The AABB made the minor crossmatch unnecessary in year ______

Answer 7

1976

Question 8

Computer Crossmatch

(YEAR)

Answer 8

1990

Question 9

Crossway mixing of donor and recipient blood components

Answer 9

Crossmatch

Question 10

A crossmatch is interpreted as compatible when ____ and ____ are present in testing

Answer 10

No agglutination
No hemolysis

Question 11

A crossmatch is interpreted as incompatible when ____ and ____ are present in testing

Answer 11

Agglutination
Hemolysis

Question 12

One unit of RBCs should increase the hematocrit by ___ and the hemoglobin by ____

Answer 12

3%
1g/dL

Question 13

Patient samples for compatibility testing may be ______

Answer 13

Serum or Plasma

Question 14

Tubes that are commonly used in compatibility testing

Answer 14

Red top (NO ANTICOAGULANT)
EDTA

Question 15

Preferred sample for compatibility testing

Answer 15

Plasma

Question 16

AGE OF THE SAMPLE

patient samples and a segment from the donor unit used for crossmatching must be stored for at least ____ days

Answer 16

7 days

Question 17

Mechanical hemolysis may be caused by

Answer 17

Use of small gauge needles

Trauma to a small vein

Forcing of blood into the tube through a small needle

Addition of blood to a partially clotted sample

Question 18

Mechanical hemolysis can mask the detection of antibody induced hemolysis in some examples of _____

Answer 18

ABO
P1
Lewis
Kidd
Vel system antibodies

Question 19

Responsible for confirming the correct ABO labeling of all donor blood received from the donor center if the units were not previously confirmed.

Answer 19

Transfusing Facility

Question 20

It is performed by making a red cell suspension of the donor blood from a segment attached to the donor bag.

Answer 20

Retyping

Question 21

TRUE OR FALSE

Platelets and plasma do not require retyping.

Answer 21

eurT

Question 22

Pretransfusion testing on the recipient’s sample includes the determination of the patients _____, _____, and ______

Answer 22

ABO and D phenotype
Antibody Screen
Crossmatch

Question 23

Are terms used to describe the detectable ABO red cell antigens

Answer 23

ABO Phenotype
ABO type
ABO group

Question 24

2 Main functions of crossmatching

Answer 24

Final check of ABO compatibility between donor and recipient
Detects the presence of an antibody in the patient’s serum.

Question 25

Two parts of crossmatch testing

Answer 25

Major Cross Match (PSDR) Minor Cross Match (PRDS)

Question 26

Detects antibodies in the serum of the donor

Answer 26

Minor crossmatch

Question 27

Detects antibodies in the serum of the recipient

Answer 27

Major crossmatch

Question 28

What are the other methods of cross matching

Answer 28

Saline cross match High protein tube test Ortho broad spectrum compatibility Enzyme method of crossmatch

Question 29

Same with saline tube test, with the addition of _______ which will detect many of the RH - Hr antibodies (2 Answers)

Answer 29

High Protein Tube Test 22% Bovine Albumin

Question 30

Enzymes employed including bromelain, trypsin, papain, and ficin

Answer 30

Enzyme method of crossmatch

Question 31

Give 3 phases of Ortho Broad Spectrum Compatibility

Answer 31

Protein/Room Temperature Phase Thermo/Incubated phase Anti globulin/Coomb's Phase

Question 32

ORTHO BROAD SPECTRUM COMPATIBILITY TEST Detect the 3 major types of antibodies including

Answer 32

Saline Albumin Antihuman globulin

Question 33

What phase detects antibodies that react optimally at 37 deg C

Answer 33

Thermo Phase

Question 34

Suspended in saline to detect saline agglutinins (_____ antibodies)

Answer 34

Saline cross match IgM

Question 35

______ ensures that only ABO compatible whole blood or red cells are selected for transfusion.

Answer 35

Computer system

Question 36

ADVANTAGES of computer crossmatch

Answer 36

Decrease workload Reduced sample volume for testing Reduced exposure of personnel to blood samples Better use of blood inventory

Question 37

SELECTION OF ABO DONOR UNITS If the component for transfusion contains ___ mL or more of _____, the donor's ____ must be _____ with the recipient's ______

Answer 37

2 mL Red cell Red cell ABO Compatible Plasma

Question 38

In the transfusion of plasma products, the ABO antibodies in the transfused plasma should be compatible with the recipient's _____

Answer 38

Red cell

Question 39

D positive blood components should be selected for ____ recipients

Answer 39

D positive

Question 40

If the recipient has a clinically unexpected antibody, the recipient should receive _____ blood

Answer 40

Antigen negative

Question 41

D negative recipient's should receive ____ that are _____ to avoid immunization to the D antigen, especially women of childbearing age.

Answer 41

RBCs D Negative

Question 42

______ and ______ propose a policy for the selection of suitable donor units for recipients with blood group antibodies using information about the antibody's history of clinical significance and availability of compatible blood

Answer 42

Poole and Daniels

Question 43

Transfusion of donor units that are crossmatch compatible with anti M and anti N is a generally accepted practice, provided that the antibodies are not reactive at what temperature?

Answer 43

37 Deg C

Question 44

In emergency situations, _____ are issued uncross matched inf the patient's ABO group is unknown.

Answer 44

Group O RBCs

Question 45

After the specimen is in the blood bank, the most important test to complete is ____ and _____

Answer 45

ABO and D phenotyping

Question 46

If _______ are in short supply. they should be reserved preferentially for emergency release to women younger than or of childbearing age.

Answer 46

Group O D negative RBCs

Question 47

May be substituted for emergency release to men and to women older than childbearing age

Answer 47

Group O, D positive RBCs

Question 48

Defined as a total volume exchange of blood t hrough transfusion within a 24 hour period, wether in an infant or in an adult patient

Answer 48

Massive Transfusion

Question 49

Blood donated by the prospective recipient for later use

Answer 49

Autologous blood

Question 50

Infants younger than _____ old are unable to produce their own antibodies

Answer 50

4 months

Question 51

At ___ - ____ old of age, infant begin producing their own ABO antibodies and become capable of producing antibodies.

Answer 51

4-6 months

Question 52

Contains almost no red cells and do not need to be crossmatched

Answer 52

Plasma Platelet concentrates Cryoprecipitate

Question 53

Blood component prepared from whole blood that contains only the plasma portion of whole blood and is frozen after separation

Answer 53

Plasma

Question 54

Platelets obtained from a whole blood donation contain a minimum of ________ platelets

Answer 54

5.5x1010

Question 55

Blood component recovered from a controlled thaw of fresh frozen plasma

Answer 55

Cryoprecipitate

Question 56

In cryoprecipitate, the cold insoluble precipitate is rich in coagulation factor ____, _____, ____, and ______

Answer 56

VIII von Willebrand's factor XIII Fibrinogen

Question 57

Apheresis procedure in which the platelets are removed from adonor, and remaining red cells and plasma are returned,

Answer 57

Apheresis Platelets

Question 58

Granulocyte Concentrates: Contains a minimum of _____ granulocytes.

Answer 58

1.0x101 granulocytes