Blood dilution Flashcards
(76 cards)
1
Q
reported as percentage
A
relative count
2
Q
shows the real count/ number
A
absolute count
3
Q
A
4
Q
•Prior to blood cell counting using the ______, blood is first diluted with specific diluting fluids
A
Improved Neubauer counting chamber
5
Q
RBc dilution if blood ratio
A
1:200
6
Q
WBC dilution of blood ratio
A
1:20
7
Q
PLT
A
1:100
8
Q
Devices used first cell count
A
Thoma Pipettes, Improved Neubauer Hemacytometer, Compound microscope
9
Q
A
10
Q
RBC Pipet markings
A
0.5/1.0/101
11
Q
WBC markings
A
0.5/1.0/11
12
Q
RBc size of bulb
A
large
13
Q
WBC size of bulb small
A
small
14
Q
rbc color of bead
A
red
15
Q
wbc color of bead
A
white
16
Q
rbc volume in bulb
A
100
17
Q
wbc volume in bulb
A
10
18
Q
RBC size of bore
A
smaller
19
Q
WBC size of bore
A
bigger
20
Q
mark to which blood is drawn (RBC)
A
0.5 mark
21
Q
mark to which blood is drawn (Plt count)
A
1 mark
22
Q
the mark to which diluting fluid is drawn
A
101 mark
23
Q
area to where both blood and diluting fluid combine
A
Bulb
24
Q
mixes the contents; identifier of the type of thoma pipette
A
Red bead
25
to which diluting fluid is drawn (wbc)
11 mark
26
to which blood is drawn (wbc)
0.5 mark
27
28
Red blood cell count diluting fluids
Isotonic solutions: Hayem's, Gower's, or Dacie's fluid.
29
WBC Count diluting fluids
2% acetic Acid; 1% Hydrochloric Acid; Turk's Diluting fluid (Containing Acetic Acid-3ml, 1 ml of aqueous gentian violet, and 100 ml distilled water)
30
platelet count diluting fluid
•1% ammonium oxalate; Rees-Ecker fluid (Containing 3.8 grams of sodium citrate, 0.2 ml of 40% formaldehyde, 0.1 gram of brilliant cresyl blue in 100 ml aqueous solution)
31
DILUTING FLUIDS
• Cheap and Economical
• Stable, Easy to Secure and Prepare
• With Preservative Action
• High Specific Gravity
• With Buffer Action
• Non-Allergenic / Non-corrosive
• WBC Diluting Fluid must be hypotonic
• RBC Diluting Fluid must be Isotonic
32
device used to count erythrocyte, leukocyte, and platelets, present in blood as well as other body fluids
hemacytometer
33
Each chamber has a total measurement of
9mm² (Subdivided into 9 squares that measures 1mm² each)
34
35
Refers to the routine procedure that gives an approximation of the total number of leukocytes in the circulation
WBC Count
36
Function of the WBC is to
provide immunity- the ability to resist infection
37
In a normal adult, WBC ranges about
4,000-11,000/mm3
38
A Count above normal can is called
Leukocytosis
39
leukocytosis PHYSIOLOGIC CAUSE
- Exercise
- Stress
- Obstetric labor
- Anesthesia
40
leukocytosis parhologic causes
-Infection
-Hematologic Disorder (leukemia)
41
counts below normal
Leukopenia
42
Leukopenia causes
• Viral Infection
• ionizing Radiation
• Chemicals
• Drugs
• Hematologic problems (aplastic anemia)
43
For RBC Counting, Blood is diluted with
isotonic solution
44
WBC Dilution Ratio
1:20
45
WBC Dilution Factor
20
46
WBC Blood Units
0.5
47
WBC Bulb Units
10
48
Blood is diluted with either:
-2% acetic Acid
- 1% Hydrochloric Acid
-Turk’s Diluting fluid (containing acetic acid-3ml, 1
ml of aqueous gentian violet, and 100 ml distilled
water
49
When WBC is markedly elevated (100-300 x10⁹/L)
the dilution can be increased
1:100
50
When WBC is also below 3 x10⁹/L, the dilution can be
reduced to
1:10
51
Difference between the total cells counted on each
side should agree
<10%
52
are resistant to lysis during blood
dilution and are counted as WBC in the
hemacytometer
Nucleated RBC
53
If more than ___ NRBC are seen in the blood smear
during differential count, the WBC count must be
corrected.
5
54
Cells Touching the _____ must be
counted.
top and left lines
55
Cells touching the _____ shall be
ignored.
bottom and right lines
56
For RBC Counting, Blood is diluted with
isotonic
solution
57
solution with equal concentration of
solutes within cells
isotonic solution
58
RBC Diluting Fluids w/ components:
Hayem’s Fluid
Gower’s Fluid
Dacie’s Fluid
59
1. HAYEM’S FLUID
• Sodium Chloride(Provides isonicity) 1 g
• Sodium Sulfate (Prevents Rouleux) 5 g
• Mercuric chloride (antiseptic) 0.5 g
• Distilled Water (Solvent) 200 ml
60
Provides isonicity
Sodium Chloride
61
Prevents Rouleux
Sodium Sulfate
62
antiseptic
Mercuric chloride
63
Solvent
Distilled Water
64
2. DACIES’S FLUID
• Trisodium Citrate- 3.13 g
• Formaldehyde (37%) 1 ml
• Distilled water 100 ml
65
3. GOWER’S FLUID
• Isotonic solution containing 12.5 g of sodium sulfate
and 33.3 g of glacial acetic acid in 200 ml distilled
water
66
• Isotonic solution containing 12.5 g of sodium sulfate
and 33.3 g of glacial acetic acid in 200 ml distilled
water
GOWER’S FLUID
67
RBC Dilution ratio
1:200
68
RBC Dilutionnfactor
200
69
RBc Blood up to
0.5 mark
70
RBc fluid up to
101 mark
71
For patients with ____, increase the
dilution. For _____, reduce the dilution (1:100)
polycythemia
anemia
72
are important in helping diagnose
bleeding disorders.
Platelet counts
73
Platelets Function primarily in
hemostasis and maintaining capillary integrity
74
Increased Platelet Count:
Thrombocytosis
75
Decreased platelet count:
Thrombocytopenia
76
