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Flashcards in Blood+Tissue Protozoa Deck (50)
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1

List some blood and tissue protozoal diseases

Malaria, Toxoplasma, Trypanosoma, Leishmania

2

Malaria parasite

Plasmodium spp:
P. falciparum, P. vivax, P. ovale, P. malariae, P.
Knowlesii
(similar life cycles)

3

Malaria protozoan carrier

Anopheles mosquito

4

Malaria transmission

Person to person via mosquito
Shared needles by drug users
Blood transfusion
Organ transplant

5

Define - Epidemic determinant factors

Environmental factors that help the parasite to survive

6

Epidemic determinant factors for Plasmodium spp

(a) Drought
(b) Elevated drug resistance
(c) Abnormal temperature
(d) Land pattern changes
(e) High malnutrition rates

7

How is drought an epidemic determinant factor for Plasmodium spp

Rainfall in usually dry area creates breeding sites

8

How is elevated drug resistance an epidemic determinant factor for Plasmodium spp

No effective antiparasitic means more spread of Plasmodium spp.

9

How is abnormal temp an epidemic determinant factor for Plasmodium spp

hot temp -climate change allows for optimal survival of mosquito species

10

How is land pattern changes an epidemic determinant factor for Plasmodium spp

People moving from country-town, less trees due to buildings, carbon emissions

11

How is high malnutrition rates an epidemic determinant factor for Plasmodium spp

anorexic + obese =malnourished b/c abnormal nutrition, easier for parasite to proliferate in malnourished host

12

Does each type of pathogen have virulence factors?
What makes it difficult for pathogens to invade?

Each pathogen has virulence factors - fungi, parasite, bacteria, virus
-If the host factors (normal flora etc) are intact it is difficult for pathogens to invade and overwhelm

13

Virulence factors of Plasmodium spp

-Antigenic variations (antigens on spp can change; fool host cell into thinking it isn’t a foreign pathogen)

-Cytoadherence of infected rbc (site adherence factor, allows pathogen infect rbc
->RBC well protected but spp overcome this

14

Malaria life cycle

1. Sporogenic cycle -> mosquito ingests gametocytes (male+female) via human
2. Gametocytes form macro and microgametes.
3. Develop ookinetes then oocysts,
4. They get to cavity of mosquito, burst open and releases sporozoites
5. Sporozoites in saliva which passes to human via bite (infective stage)

6. Sporozoites move to liver then form another cycle called exo-erythrocytic cycle.
7. Takes place outside rbcs.
8. Schizont (mature sporozoite) ruptures then goes to rbcs.

9. Now Erythrocitic cycle. (rbc stage)
10. It will enter rbcs, rbc will rupture and schizonts go to liver or blood.

P vivax and P ovale can remain dormant as hypnozoites in liver.

15

Lab ID - Malaria

Microscopy: Thick & Thin Blood film staining
Stained with Giemsa
-looking for the small trophozoites (ring) in the cells or the banana shaped gametocytes.
Gold std. id method
(purple capped tube - malaria)

Serological methods: to detect Abs is only used
for screening in blood donors. Ab may not be
present in acute infection.

16

Fluorescent dye technique - Malaria ID

Fluorescent dyes that stain nucleic acids have been used in the detection of blood parasites.

• Kawamoto technique - blood smears on a slide are stained with acridine orange and examined with either a fluorescence microscope or a light microscope adapted with an interference filter system.

• Differential staining of nuclear DNA in green and of cytoplasmic RNA in red, which allows recognition of the parasites.

17

Additional Diagnostic Tests - Malaria

Ag detection (rapid test):
(a) Plasmodium lactate dehydrogenase-pLDH,
(b) Histidine rich parasite-HRP2,
(c) Pan-Plasmodium LDH,
(d) Quantitative buffy coat-QBC

Molecular tests: PCR

18

Does Quantitative Buffy Coat Method have good sensitivity in detecting malarial parasites?

The QBC method is reported to have a good sensitivity for detection of malaria parasites.

19

Treatment for blood and tissue protozoa

Tissue Schizonticides: - Primaquine

• Blood Schizonticides: - Chloroquine-susceptible -
Chloroquine phosphate; Chloroquine-resistant -
Mefloquine, Quinine sulfate, Pyremethamine-
Sulfadoxine; Doxycycline, Halofantrine, Artemisinin
(Quinghaosu), Atovaquone-proguanil

•Gametocytocidal: - Quinidine gluconate, Artesunate,
Quinine dihydrochloride, Artemether

20

Malaria species - range in severity of anemia and list symptoms

Anaemia usually results mildly in P. ovale, moderately in P. vivax and P. malariae and very severe in P. falciparum.

Symptoms:
- Quotidian/recurring fever, nausea, vomiting,

21

Malaria - complications

• CNS involvement (cerebral malaria) - may or may not be in P. vivax, P. ovale or P. malariae type but in P. falciparum, there is and usually very severe.

• Nephrotic syndrome does not occur in P. ovale, may occur in P. vivax, mildly in P. falciparum but usually always in P. malariae

• Hypoglycaemia; Pulmonary oedema & Respiratory distress;
Metabolic (Lactic) Acidosis; Malaria of Pregnancy

22

Malaria prevention

Chemoprophylaxis (killing effect against parasite, either within the erythrocytes/ hepatocytes)
Chloroquine phosphate, Mefloquine,
Doxycycline, Atovaquone-proguanil

• PPE & Avoidance: Protective clothing, eradication of
mosquitoes and their breeding sites, use of mesh in
houses, use of insect repellants; health education

• Vaccines – still being developed

23

Parasitic flagellate protozan diseases from family Trypanosomatidae

Trypanosoma and Leishmania

24

Why is Trypanosoma and Leishmania called hemoflagellates?

• Because these protozoans require hematin obtained
from blood hemoglobin for aerobic respiration

25

Digenetic life cycle

Two host life cycle

26

Trypanosoma and Leishmania have a digenetic life cycle involving insects and vertebrates (T/F)

True

27

Hemoflagellates have up to 8 life cycle stages that differ in flagella placement (T/F)

True

28

Two stages (hemoflagellates) in invertebrates

Amastigote + Trypomastigote
---in vertebrates

29

Three stages

promastigote, paramastigote, and epimastigote
—in invertebrate hosts.

30

List two stages for Leishmania and Trypanosoma

Leishmania (promastigote (insect->infective stage), amastigote (mammalian stage))

Trypanosoma brucei (epimastigote (insect stage), trypomastigote (mammalian stage))