BMS 127 Ch. 4 Microscopy, Staining and Classification Flashcards
A nanometer is _________ than a micrometer.
1000 times smaller
Which of the following is the smallest?
a. decimeter
b. millimeter
c. nanometer
d. micrometer
nanometer
Resolution is best described as __________.
the ability to distinguish between two adjacent objects.
Curved glass lenses ______ & _______ the light.
refract; bend
Which of the following factors is important in making an image appear larger?
a. thickness of the lens
b. curvature of the lens
c. the speed of light passing through the lens
d. all of the above
all of the above
Which of the following is different between light microscopy and transmission electron microscopy?
a. magnification
b. resolution
c. wavelengths
d. all of the above
all of the above
Which type of microscopy produces a three-dimensional image with a shadowed appearance?
differential interference contrast microscope
What type of microscope combines the greatest magnification with the best resolution?
confocal microscope
What is another name for negative stains such as eosin?
capsule stains
In the binomial system of nomenclature, which term is always written in lowercase letters?
specific epithet
If an objective magnifies 40X, and each binocular lens magnifies 15X, the total magnification of the object being viewed is _____.
600X
The type of fixation developed by Koch for bacteria is ________.
heat fixation
Immersion oil ________(increases/decreases) the numerical aperture, which ________(increases/decreases) resolution because ________(more/fewer) light rays are involved.
increases; increases; more
____________ refers to the differences in intensity between two objects.
Contrast
Cationic chromophores such as methylene blue are ionically bound to ____________ (positively/negatively) charged chemicals such as DNA and proteins.
negatively
