BMS 127 Ch. 7 Microbial Genetics Flashcards
Which of the following is a true statement concerning prokaryotic chromosomes?
a. They typically have two or three origins of replication.
b. They contain single-stranded DNA
c. They are located in the cytosol
d. They are associated in linear pairs
c. They are located in the cytosol
Which of the following is most likely the number of base pairs in a bacterial chromosome?
a. 4,000,000
b. 4000
c. 400
d. 40
a. 4,000,000
What is a plasmid?
Extrachromosomal DNA
What the function of a histone?
to form ionic bonds with eukaryotic DNA and stabilizes
Nucleotides used in the replication of DNA
a. carry energy.
b. are found in four forms, each with a deoxyribose sugar, a phosphate, and a base
c. are present in the cytosol of cells as triphosphate nucleotides
d. all of the above
d. all of the above
What molecule functions as a “proofreader” for a newly replicated strand of DNA?
DNA Polymerase III
What is the addition of -CH3 to a cytosine nucleotide after DNA replication called?
methylation
In translation, what is the biding site through which tRNA molecules leave?
E site
What does the Ames test use to reveal potential mutagens?
auxotrophs and liver extract
What method of DNA repair involves enzymes that recognize and correct nucleotide errors in unmethylated strands of DNA?
mismatch repair
Which of the following is NOT a mechanism of natural genetic transfer and recombination?
a. transduction
b. transformation
c. transcription
d. conjugation
c. transcription
Cells that have the ability to take up DNA from their environment are said to be ________.
competent
Conjugation requires a ________ extending from the surface of a cell.
sex pilus
What genes are “jumping genes”?
transposons
Although cells P and Q are totally unrelated, cell Q receives DNA from cell P and incorporates this new DNA into its chromosome. This is a process called ________.
horizontal gene transfer