BMS 127 Ch. 7 Microbial Genetics Flashcards

0
Q

Which of the following is a true statement concerning prokaryotic chromosomes?

a. They typically have two or three origins of replication.
b. They contain single-stranded DNA
c. They are located in the cytosol
d. They are associated in linear pairs

A

c. They are located in the cytosol

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1
Q

Which of the following is most likely the number of base pairs in a bacterial chromosome?

a. 4,000,000
b. 4000
c. 400
d. 40

A

a. 4,000,000

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2
Q

What is a plasmid?

A

Extrachromosomal DNA

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3
Q

What the function of a histone?

A

to form ionic bonds with eukaryotic DNA and stabilizes

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4
Q

Nucleotides used in the replication of DNA

a. carry energy.
b. are found in four forms, each with a deoxyribose sugar, a phosphate, and a base
c. are present in the cytosol of cells as triphosphate nucleotides
d. all of the above

A

d. all of the above

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5
Q

What molecule functions as a “proofreader” for a newly replicated strand of DNA?

A

DNA Polymerase III

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6
Q

What is the addition of -CH3 to a cytosine nucleotide after DNA replication called?

A

methylation

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7
Q

In translation, what is the biding site through which tRNA molecules leave?

A

E site

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8
Q

What does the Ames test use to reveal potential mutagens?

A

auxotrophs and liver extract

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9
Q

What method of DNA repair involves enzymes that recognize and correct nucleotide errors in unmethylated strands of DNA?

A

mismatch repair

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10
Q

Which of the following is NOT a mechanism of natural genetic transfer and recombination?

a. transduction
b. transformation
c. transcription
d. conjugation

A

c. transcription

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11
Q

Cells that have the ability to take up DNA from their environment are said to be ________.

A

competent

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12
Q

Conjugation requires a ________ extending from the surface of a cell.

A

sex pilus

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13
Q

What genes are “jumping genes”?

A

transposons

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14
Q

Although cells P and Q are totally unrelated, cell Q receives DNA from cell P and incorporates this new DNA into its chromosome. This is a process called ________.

A

horizontal gene transfer

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15
Q

Which of the following is part of each molecule of mRNA?

a. palindrome
b. codon
c. anticodon
d. base pair

A

b. codon

16
Q

A nucleotide is composed of

a. a five carbon sugar
b. phosphate
c. a nitrogenous base
d. all of the above

A

d. all of the above

17
Q

In DNA, adenine forms ________ hydrogen bonds with _________.

A

two; thymine

18
Q

What is a sequence of nucleotides formed during replication of the lagging DNA strand?

A

an Okazaki fragment

19
Q

Which of the following is not part of an operon?

a. operator
b. promoter
c. origin
d. gene

A

c. origin

20
Q

Repressible operons are important in regulating prokaryotic _________.

A

RNA transcription

21
Q

What does transcription produce?

A

RNA molecules

22
Q

What does ligase play a major role in?

A

lagging strand replication

23
Q

Before mutations can affect a population permanently, they must be

a. lasting
b. inheritable
c. beneficial
d. all of the above

A

d. all of the above

24
Q

The three steps in RNA transcription are ________, ________ and _______.

A

initiation; enlongation; termination

25
Q

A triplet of mRNA nucleotides that specifies a particular amino acid is called a ________.

A

codon

26
Q

Three effects of point mutations are ______, _______ and ________.

A

silence; missense; and nonsense

27
Q

Insertions and deletions in the genetic code are also called _______ mutations.

A

frameshift

28
Q

An operon consists of _______, ______ and _________ and is associated with a regulatory gene.

A

promoter; operator; series of genes

29
Q

In general, __________ operons are inactive until the substrate of their genes’ polypeptides is present.

A

inducible

30
Q

A daughter DNA molecule is composed of one original strand and one new strand because DNA replication is ____________.

A

semiconservative

31
Q

A gene for antibiotic resistance can move horizontally among bacterial cells by ________, __________ and ____________.

A

transformation; transduction; bacterial conjugation

32
Q

____________ are nucleotide sequences containing palindromes and genes for proteins the cut DNA strands.

A

Transposons

33
Q

_______ _______ is a recombination event that occurs during gamete formation in eukaryotes.

A

Crossing over

34
Q

______RNA carries amino acids.

A

Transfer

35
Q

______ RNA and _______ RNA are antisense; that is, they are complementary to another nucleic acid molecule.

A

Short interference; micro