Bone marrow

Question 1

Which processes take place in the bone marrow contributing to peripheral blood?

Answer 1

Erythropoiesis 
Granulopiesis 
Megakaryopoiesis 
thrombopoiesis 
thrombopoiesis 
lymphopoiesis

Question 2

Where does hematopoiesis take place in prenatal and postnatal?

Answer 2

*Postnatal: Yolk-sac>liver and spleen.
From 4 months : Bone marrow >liver > spleen

Postnatal :Tibia ,Femur BEFORE TURNING 30 YRS
After turning 30: Vertebrae >Sternum > Rib

Question 3

T/F: After puberty, marrow contains a significant number of adipocytes, and these increase with age.Average adult marrow cellularity = 40-60%

Answer 3

False: After infancy

Question 4

How do we diagnose diseases in the bone marrow?

Answer 4

> Bone marrow biopsy

Question 5

What is the best site for marrow biopsy?

Answer 5

*Illiac crest and never sternum

Question 6

What time of sample is obtained?

Answer 6

• Bone aspirate

* Bone marrow trephine

Question 7

Uses of the aspirate?

Answer 7

*To provide cells for cytological examination
Procedure: Spread sample on a slide, stain, and exam microscopically to determine the morphology

• Provide anticoagulated specimen for flow cytometry, cytogenetics, and culture
• DNA PCR and FISH can be done on spread marrow / anticoagulated specimen
• RT-PCR requires anticoagulant specimens in a special medium

Question 8

Uses of the trephine biopsy?

Answer 8

• Used to see the architecture of the bone marrow rather than individual cells :
• granulomas,infiltrating neoplasm eg lymphoma ,carcinoma,marrow necrosis fibrosis