Brightfield Microscopy Flashcards
A microscope that allows light rays to pass directly to the eye without being deflected by an intervening
opaque plate in the condenser is called
brightfield microscope
microscopes, basic frame structure
Head
Arm
Base
The horizontal platform that supports the microscope slide is called the ___. Note that it has a clamping device, the ____, which is used for holding and moving the slide around on the stage.
stage; stage adjustment
This objective lens provides the highest magnification
Oil Immersion
This objective lens provides the second-highest magnification
High Dry
This objective lens provides the lowest magnification.
Low Power
This objective lens has the shortest working distance.
Oil Immersion
The coarse focus knob should be adjusted only when using this objective lens.
Low Power
This lens collects and focuses light from the lamp onto the specimen on the slide.
Condenser
This lens, also known as the eyepiece, often comes in pairs.
Diopter adjustments can be made to this lens.
Ocular
A ___ is used to regulate light passing through this Condenser.
diaphragm
True/False
Acetone is the safest solvent for cleaning an objective lens.
False;
use Green soap with warm water
Xylene is universally acceptable
Alcohol
True/False
Only lint-free, optically safe tissue should be used to wipe off
microscope lenses.
True
True or False
total magnification capability of a light microscope is only limited by the magnifying power of the lens system.
False; Magnification(10x) x Power of Ocular Lense(100x) = Total Magnification(1000)
True or False
The coarse focus knob can be used to adjust the focus when using any of the objective lenses
False; not in High-Dry Objective Lense
Once focus is achieved at one magnification, a higher power objective lens can be rotated into position without fear of striking the slide.
True
The resolving power of a microscope is a function of
the numerical aperture of the lenses.
the wavelength of light.
The coarse and fine focus knobs adjust the distance between
the stage and the condenser lens.
A microscope that maintains focus when the objective magnification is increased is called
refractive
The total magnification achieved when using a 100x oil immersion lens with 10x binocular eyepieces is
1000x
The most useful adjustment for increasing image contrast in low power magnification is
closing down the diaphragm
Before the oil immersion lens is rotated into place, you should
center the object of interest in the preceding lens.
place a drop of oil on the slide.
limit of resolution of the typical light microscope
microscope, the limit is .2 um
the limit of resolution of the unaided human eye is about
.2 mm
