Brucella and Legionella

Question 1

Brucella is normal flora of (2)

Answer 1

urinary tract and GI tract of sheep/goat

Question 2

Brucella characteristics (4)

Answer 2

Non Motile,
Non Encapsulated,
strict aerobe,
intracellular (inside phagocyte)

Question 3

Brucella
Catalase
Oxidase
Urease
Nitrate

Answer 3

Catalase (+),
Oxidase (+)
Urease (+)
Nitrate (+)

Question 4

Brucella growth factor

Answer 4

Erythritol (placental tissue)

Question 5

Brucella Incubation period

Answer 5

2-5 months

Question 6

Brucella Specimen (4)

Answer 6

blood
bone marrow
tissues,
bronchoalveolar lavage

Question 7

Brucella category

Answer 7

Class B of bioterrorism

Question 8

B. abortus (2)

Answer 8

● Bang’s bacillus or Malta fever - cattles
● Less invasive

Question 9

B. suis (3)

Answer 9

● Swine or pigs
● Highly invasive
● Can cause necrosis

Question 10

B. canis (2)

Answer 10

● Dogs
● Mild to localized infections

Question 11

B. melitensis diseases (7)

Answer 11

Undulant fever,
Malta fever,
Gibraltar fever,
Mediterranean fever,
Rock fever,
Gastric Remittent fever,
Crimean fever Maltese fever

Question 12

Most serious and Debilitating infection

Answer 12

B. melitensis

Question 13

B. melitensis Optimal specimen

Answer 13

Blood

Question 14

B. melitensis Definitive Test

Answer 14

Bone marrow culture

Question 15

Rose Bengal Test

Answer 15

B. melitensis

Question 16

B. melitensis media (4)

Answer 16

○ TSB
○ Castaneda Broth - best medium
○ BBA
○ W media - specific

Question 17

Facultative intracellular pathogen

Answer 17

LegionellaLegionella

Question 18

Legionella
Motility,
Catalase
Weakly Oxidase
Gelatinase

Answer 18

Motile
Catalase (+)
Weakly Oxidase (+);
Gelatinase (+)

Question 19

Legionella specimens (4)

Answer 19

sputum,
pleural fluid,
blood,
lung biopsy

Question 20

Most common human pathogen

Answer 20

L. pneumophila

Question 21

Where can you find L. pneumophila (5)

Answer 21

Air Conditioning ducts,
cooling towers,
plumbing system,
shower heads,
nebulizers

Question 22

L. pneumophila disease (2)

Answer 22

● Legionnaire’s disease
● Pontiac fever

Question 23

broad strict pneumonia

Answer 23

Legionnaire’s disease

Question 24

resembles allergic (non fatal)

Answer 24

Pontiac fever

Question 25

L. pneumophila MOT

Answer 25

air-borne

Question 26

L. pneumophila culture

Answer 26

○ BCYE - Buffered charcoal yeast extract
○ plates should be kept for 2 weeks since it’s hard to isolate
Feeley Gorman agar - brown colored

Question 27

Pittsburgh pneumonia

Answer 27

L. micdadei