[C] Chromatography

Question 1

What is chromatography used for?

Answer 1

To separate mixtures into their individual components.

Question 2

True or False: Chromatography can only be used for colored substances.

Answer 2

False

Question 3

What are the two main phases in chromatography?

Answer 3

The stationary phase and the mobile phase.

Question 4

Fill in the blank: In paper chromatography, the stationary phase is _____ paper.

Answer 4

filter

Question 5

What is the purpose of the solvent in chromatography?

Answer 5

To carry the substances through the stationary phase.

Question 6

Which factor affects the distance a substance travels in chromatography?

Answer 6

The affinity of the substance for the stationary phase versus the mobile phase.

Question 7

Multiple Choice: What is the stationary phase in thin-layer chromatography?

Answer 7

A solid adsorbent material.

Question 8

What does Rf value stand for?

Answer 8

Retention factor.

Question 9

How is Rf value calculated?

Answer 9

Distance traveled by the substance divided by distance traveled by the solvent.

Question 10

True or False: A higher Rf value indicates a greater affinity for the stationary phase.

Answer 10

False

Question 11

What is the role of a pencil in paper chromatography?

Answer 11

To mark the baseline without interfering with the separation.

Question 12

Fill in the blank: The _____ phase is usually a liquid in liquid chromatography.

Answer 12

mobile

Question 13

What type of substances can be separated using gas chromatography?

Answer 13

Volatile substances.

Question 14

Multiple Choice: In gas chromatography, what is the mobile phase?

Answer 14

A gas.

Question 15

What is the significance of the separation in chromatography?

Answer 15

It allows for the identification and analysis of individual components.

Question 16

True or False: Chromatography can be used for both qualitative and quantitative analysis.

Answer 16

True

Question 17

What is the purpose of spotting in chromatography?

Answer 17

To apply a small amount of the mixture onto the stationary phase.

Question 18

Fill in the blank: In chromatography, substances with a _____ affinity for the mobile phase travel further.

Answer 18

higher

Question 19

What can affect the results of chromatography?

Answer 19

Temperature, solvent choice, and the type of stationary phase.

Question 20

Multiple Choice: Which of the following is NOT a type of chromatography?

Answer 20

Magnetic chromatography.

Question 21

What is the first step in a chromatography experiment?

Answer 21

Prepare the stationary phase.

Question 22

True or False: Chromatography can be performed in a closed system.

Answer 22

True

Question 23

What should be recorded during a chromatography experiment?

Answer 23

The distance traveled by the solvent and each substance.

Question 24

Fill in the blank: The _____ of the solvent front should be marked after the experiment.

Answer 24

position

Question 25

What is one application of chromatography in real life?

Answer 25

Testing for drugs in urine.

Question 26

Multiple Choice: Which type of chromatography is best for separating complex mixtures?

Answer 26

High-performance liquid chromatography (HPLC).

Question 27

What does the term 'baseline' refer to in chromatography?

Answer 27

The starting line where the mixture is applied.

Question 28

True or False: The results of chromatography can be visualized using UV light.

Answer 28

True

Question 29

What is one disadvantage of paper chromatography?

Answer 29

Limited resolution for closely related compounds.

Question 30

Fill in the blank: Chromatography is often used in _____ analysis.

Answer 30

chemical

Question 31

What is the main advantage of gas chromatography?

Answer 31

High sensitivity and resolution for volatile compounds.