Carb Catabolism, Fermentation, Phenyl Red/MVRP Flashcards
(22 cards)
process that degrades compounds (glucose/) to release energy; cell use the energy to synthesize ATP
catabolism
group of organic compounds that include sugar and starches; contain CH2O
carbohydrates
Carbohydrate functions
energy source/storage, cell wall structural component
simple sugar with 3-7 carbon atoms
monosaccharide
type of carbohydrate composed of 2-20 monosaccharide units
Oligosaccharide
type of carb hat consist of 20+ monosaccharide molecules
polysaccharide
leaves cell to breaks down large substrates into their smaller components by addition of water
hydrolytic enzymes
process where O2 is used to make energy
oxidative catabolism
Starch is aka
amylum; which is why amylase hydrolizes starch
Bacterias that do not hydrolize salt well
Staphlycoccus and CLostridium (gram +)
_____ medium is used for glucose fermentation
OF medium
Phenyl red glucose/dextrose test
Phenyl red dye is pH indicator (6.8 to 7.4)
Phenyl red becomes yellow when the pH is
less than 6.8
phenyl red will become pink/purple when the pH is
greater than 7.4
If bacteria can break down the glucose, the ______ ______ end product will result in a be yellow color.
pyruvic acid
If bacteria cannot break down glucose, what happens to the color?
It will remain red
Enzyme needed to breakdown glucose
hexokinase
metabolic process in which an organism converts a carbohydrate, such as starch or a sugar, into an alcohol or an acid
fermentation
which test tubes indicate gas production (fermentation end product)?
Durham/inverted test tubes
test used to distinguish organisms that produce large amounts of acid from glucose & orgs that produce neutral product acetoin
MVRP test
if large amount of acid is produced, MV will remain _______
red
if neutral products are produced, it will turn _____
yellow
