Protein Catabolism/Gelatin/ H2S/ Indole

Question 1

subunits of proteins

Answer 1

amino acids

Question 2

amino acids consists of

Answer 2

carbon, hydrogen , oxygen, nitrogen and sometimes sulur

Question 3

amino acids are joined by & form small chains ____ or larger molecule _____

Answer 3

peptide bonds; peptide chain, polypeptide

Question 4

chemical reactions cells use to synthesize & assemble subunits into their macromolecules using ATP for energy

Answer 4

anabolism/biosynthesis

Question 5

enzyme that breaks down gelatin; breakdown of gelatin results in

Answer 5

gelatinase; smaller polypeptides, then into amino acids

Question 6

before an amino acid is used as a carbon/energy source, what must be removed?

Answer 6

The amino group

Question 7

removal of the amino group

Answer 7

deamination

Question 8

Deamination process

Answer 8

Amino group is converted to ammonia, which is excreted from the cell

Question 9

Deamination results in the formation of what?

Answer 9

organic acid

Question 10

how cam deamination be tested?

Answer 10

colored ferric ion complex will be formed with acid form; OR testing for ammonia with Nessler’s reagent , which will turn a deep yeloow

Question 11

removal of CO2 from amino acid

Answer 11

decarboxylation

Question 12

Bacteria with sulfur containing amino acids release?

Answer 12

Hydrogen sulfide H2S

Question 13

To detect H2S, what is added to a culture media?

Answer 13

Heavy metal salt containing Ferrous Ion