CELL BIOLOGY

Question 1

CELL CYCLE & MITOSIS MNEMONIC

Answer 1

Green—Growth & organelle duplication
Dogs—DNA replication
Can—Chromosomes condense
Mate—Middle
Producing—Pull apart
Tiny—Two nuclei
Dogs—Divide(cytoplasm and cell membrane)

Question 2

CELL CYCLE AND MITOSIS PROCESS

Answer 2

-Growth and DNA replication occur
-DNA condenses into chromosomes
-Chromosomes line up in the middle
-Chromosomes are pulled apart by fibres to opposite poles of cell
-Two nuclei divide to form round chromosomes
-Cytoplasm and Cell membrane divide to form 2 identical daughter cells

Question 3

PROCESS OF BINARY FISSION

Answer 3

-Circular DNA & plasmids replicate
-Cell grows & circular DNA strands move to opposite poles of the cell
-Cytoplasm begins to divide & new cells form
-Cytoplasm divides fully and two daughter cells produced with an equal number of circular DNA strands and variable number of plasmids

Question 4

RQP 02: MICROBIOLOY

Answer 4

1.Use bunsen burner on a blue flame
2. Remove lid of the bottle containing bacteria culture and flame neck of bottle through bunsen flame
3. Pass inoculating loop through the flame and use an inoculating loop to collect bacteria
4. Lift lid of agar plate at an angle and place bacteria on agar plate
5. Quickly flame the neck of the bottle again and replace the lid
6.Lift lid of agar plate at an angle and spread bacteria evenly across the agar
7.Place antibiotic soaked disks on the plate and a control disc that has been soaked in sterile water only and make not of which antiseptic is in each section of plate
8. Lightly tape the lid of the pétri dish to reduce entry of microorganisms from air and prevent anaerobic conditions
7.Incubate at 25° for a few days
9. Measure the diameter the zone of inhibition(clear zone) around the disks

Question 5

RQP 01 ; MICROSCOPY

Answer 5

1) Clip the slide onto the stage and lower stage to its lowest position
2) Select the objective lens with the lowest magnification
3)look through the eyepiece and turn the coarse focus knob until you see the object
4) Turn the focus on the fine focus knob, until object is in focus
5) Repeat steps 1-5 using an objective lens with a higher magnification to see object in greater detail

Question 6

OSMOSIS RQP

Answer 6

1.Use bunsen burner on a blue flame
2. Remove lid of the bottle containing bacteria culture and flame neck of bottle through bunsen flame
3. Pass inoculating loop through the flame and use an inoculating loop to collect bacteria
4. Lift lid of agar plate at an angle and place bacteria on agar plate
5. Quickly flame the neck of the bottle again and replace the lid
6.Lift lid of agar plate at an angle and spread bacteria evenly across the agar
7.Place antibiotic soaked disks on the plate and a control disc that has been soaked in sterile water only and make not of which antiseptic is in each section of plate
8. Lightly tape the lid of the pétri dish to reduce entry of microorganisms from air and prevent anaerobic conditions
7.Incubate at 25° for a few days
9. Measure the diameter the zone of inhibition(clear zone) around the disks