Cell Biology Lab Flashcards

1
Q

What is a P-100

A

A micropipetter

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2
Q

When do you reject Ho

A

When the P value is less than .05

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3
Q

What is a P value

A

In statistics, the P value tells you the likelihood that a random sample selected is representative of the population. The higher the P value, the higher probability that the sample is representative of the population.

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4
Q

If you have 250mg of ink mixed with 500mg of water, what is the RATIO of ink to water?

A

1:2

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5
Q

What is a normal distribution?

A

A normal distribution is a bell shaped curve that is symmetric around its mean, and is measured in units of standard deviation.

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6
Q

What test in R do you run if you have a normal distribution?

A

Shapiro test

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7
Q

What test in R do you run if the data is not normally distributed?

A

Wilcox test

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8
Q

What wavelength is the spectrophotometer set at in labs 1-3

A

600nm

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9
Q

What does it mean to reject Ho?

A

The data collected is not statistically significant to accept the null hypothesis.

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10
Q

How wide are the small squares in a hemocytometer?

A

1mm squared

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11
Q

What is Trypan Blue used for?

A

To identify dead cells

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12
Q

How does Trypan Blue enter the cell

A

Through ruptures in the cell membrane

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13
Q

What is the readout of lab 4?

A

Percentage of budding yeast cells

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14
Q

What color cells do you count in lab 4

A

White

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15
Q

What enzyme generates ethanol?

A

Alcohol dehydrogenase

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16
Q

How does ethanol affect a yeast cell?

A

It is highly toxic

17
Q

What happened to the yeast cells as the concentration of ethanol rose?

A

They died at a higher rate

18
Q

What time points were observed in lab 4 on yeast cells?

A

4hr, 4.5hr, 5hr

19
Q

What regenerates NAD+?

A

3-glycol dehydrogenase

20
Q

How are enzymes named?

A

By their function

21
Q

What is the purpose of SDS-Page?

A

To sort proteins by size

22
Q

Why do you use CBB

A

To determine if you have enough protein to work with

23
Q

How are the proteins moved through the gel

A

An electric field

24
Q

What speed do you centrifuge at

A

Max rpm (15000)

25
Q

What is the purpose of bromothymol blue

A

A dye that will stain the proteins

26
Q

What solution is the gel soaked with after taking it off the power

A

40% methanol