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Flashcards in Cell Fractionation Deck (4)
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1

What conditions are needed for cell fractionation?

• Cold. To reduce enzyme activity that might break down the organelles.
• Is of the same water potential as the tissue - to prevent organelles bursting or shrinking as a result of osmotic gain or loss of water.
• Buffered - so that the pH doesn't fluctuate. Any change in the pH could alter the structure of the organelles or affect the functioning of enzymes.

2

Homogenisation

Cells are broken up by a homogeniser.
This releases the organelles from the cell.
The resultant fluid (homogenate) is then filtered to remove and complete cells and large pieces of debris.

3

Ultracentrifugation

A method of separating the contents of a homogenised fluid of cells.

4

Order of contents of ultracentrifugation

Nuclei
Mitochondria
Lysosomes