Cell Morphology

Question 1

Largest bacterium

Answer 1

Thiomargarita Namibiensis

Question 2

Most bacteria range in what size

Answer 2

0.4 - 2 um

Question 3

3 Basic Bacteria Shapes

Answer 3

Cocci (spherical)
Bacilli (rod shaped)
Spirochetes (spiral)

Question 4

Cocci in pairs

Answer 4

Diplococci

Question 5

Cocci in chains

Answer 5

Streptococci

Question 6

Cocchi in clusters

Answer 6

Staphylococci

Question 7

Bacterial cells in four

Answer 7

Tetrad

Question 8

Cocci in groups of eight

Answer 8

Sarcinae

Question 9

Shape of bacilli with tapered, pointed ends are termed as?

Answer 9

Fusiform

Question 10

When a species differs in size and shape within a pure culture, it is termed as?

Answer 10

Pleomorphic

Question 11

Bacilli in single rods/paired

Answer 11

Diplobacilli

Question 12

Bacilli in chains

Answer 12

Streptobacilli

Question 13

Bacterium that align themselves side by side

Answer 13

palisades

Question 14

Bacterium ovoid in shape is termed as

Answer 14

Coccibacilli

Question 15

Examples of Basic dyes

Answer 15

Methylene Blue
Basic fuchsin
Crystal violet
Safranin
Malachite green

Question 16

Example of acidic dye

Answer 16

Eosin

Question 17

Type of staining that applies only single staining solution

Answer 17

Simple staining

Question 18

Basic dyes have an affinity for

Answer 18

Nuclei

Question 19

Acidic dyes have an affinity for

Answer 19

Cytoplasm

Question 20

Type of staining directed towards the forms and shapes of cells

Answer 20

Simple Staining

Question 21

Type of staining that applies 2 or more stains

Answer 21

Differential satining

Question 22

4 components of Differential Staining

Answer 22

Primary Stain
Mordant
Decolorizer
Counterstain

Question 23

Type of staining which results in bacteria as light colored bodies against a dark background

Answer 23

Negative Staining

Question 24

In Negative staining, which comes first on the slide, Organism or Stain?

Answer 24

Stain

Question 25

Examples of dyes used in Negative staining

Answer 25

India Ink Nigrosin Stain

Question 26

Gram stain was developed by who

Answer 26

Hans Christian Gram

Question 27

Who did Hans Christian Gram work with

Answer 27

Carl Friedlander

Question 28

Primary stain of Gram Staining method

Answer 28

Crystal Violet

Question 29

Mordant used in Gram staining method

Answer 29

Iodine

Question 30

Decolorizing agent used in Gram staining method

Answer 30

Alcohol/Alcoholacetone

Question 31

Counterstain used in Gram staining method

Answer 31

Safranin

Question 32

Which has a thicker cell wall, Gram+ bacterium or Gram- bacterium

Answer 32

Gram+

Question 33

What does Gram+ bacteria have that Gram- dont

Answer 33

Teichoic acid Lipoteichoic acid Thick peptidoglycan

Question 34

What do Gram- bacteria have that Gram+ dont

Answer 34

Porin Lipopolysaccharides Thin peptidoglycan

Question 35

Color of stained gram+ bacteria

Answer 35

blue to purple

Question 36

Color of stained gram- bacteria

Answer 36

pink

Question 37

Determines the Gram staining characteristics of a species

Answer 37

Cell wall structure

Question 38

May be used in gram staining instead of fixation

Answer 38

Methyl Alcohol

Question 39

If crystal violet is rinsed too vigorously...

Answer 39

Poor to No staining of Gram- organisms

Question 40

If decolorization is too vigorous or prolonged...

Answer 40

Gram+ will not stain

Question 41

If decolorization is insufficient, organisms may be...

Answer 41

Falsely Gram+

Question 42

If Counterstain (Safranin) is prolonged...

Answer 42

Gram+ will be affected

Question 43

If counterstain (safranin) is insufficient...

Answer 43

failure in staining Gram- materials

Question 44

Primary stain in Acid-Fast Staining Method

Answer 44

Carbol Fuchsin

Question 45

Penetration of dye by heat

Answer 45

Ziehl-Neelsen method

Question 46

Penetration of dye by a detergent or cold with tergitol

Answer 46

Kinyoun method

Question 47

Decolorizing agent used in Acid-Fast Staining method

Answer 47

Acidified alcohol

Question 48

Counterstain used in Acid-Fast Staining method

Answer 48

Methylene Blue

Question 49

Color of stained Acid-Fast bacteria

Answer 49

Red

Question 50

Color of stained Non Acid-Fast bacteria

Answer 50

Blue

Question 51

Acid-Fast staining is used to identify:

Answer 51

Saccharomyces Coccidian Parasites

Question 52

Fluorochrome stain used in Acid-Fast Staining used for Screening Specimens

Answer 52

Auramine-Rhodamine

Question 53

Myobacteria in Fluorescent Stain stains what color

Answer 53

Bright Orange

Question 54

Myobacteria in Kinyoun and Ziehl-Neelstain Stain stains what color

Answer 54

Red

Question 55

Background material in Kinyoun and Ziehl-Neelstain Stain stains what color

Answer 55

Blue

Question 56

A flourochrome dye that stains both Gram+ and Gram- bacteria

Answer 56

Acridine Orange

Question 57

A colorless dye that binds to cellulose and chitin

Answer 57

Calcofluor White

Question 58

Stain that may be used as a specific stain for rapid screening of clinical specimens for fungal elements

Answer 58

Calcofluor White

Question 59

Yeast cells, Pseudohyphae, and Hyphae, stains what color under Calcofluor Stain?

Answer 59

Bright Apple-Green / Blue-White

Question 60

The neutral dye used in Rapid Modified Wright-Giemsa Stain is a combination of?

Answer 60

Basic Thiazine Dyes & Acid Eosin

Question 61

Methylene Blue has traditionally been used to stain what bacterium

Answer 61

C. diptheriae

Question 62

Used to stain walls of medically important fungi grown in slide culture

Answer 62

Lactophenol Cotton Blue

Question 63

Commonly used to stain bacterial spores

Answer 63

Schaefer-Fulton Spore stain

Question 64

Primary stain used in Schaefer-Fulton Spore staining method

Answer 64

Malachite Green

Question 65

Counterstain used in Schaefer-Fulton Spore staining method

Answer 65

Safranin

Question 66

In the Schaefer-Fulton Spore staining method, Endospores stain what color

Answer 66

Green

Question 67

In the Schaefer-Fulton Spore staining method, Bacterial cells stain what color

Answer 67

Pink / Red