(LAB) Specimen Collection

Question 1

When should specimen be collected

Answer 1

Acute phase of illness

Question 2

What to use when patient is on antimicrobial

Answer 2

Thiol Broth

Question 3

Sulfonamide can be neutralize by addition of?

Answer 3

P-amino benzoic acid

Question 4

Penicillin can be counteracted by adding?

Answer 4

Penicillinase

Question 5

Components of Bacto-Penase Broth

Answer 5

Brain Heart Infusion
Penicilinase
P-amino benzoic acid

Question 6

Preservative for Urine

Answer 6

Boric Acid

Question 7

Preservative for stool

Answer 7

Polyvinyl alcohol & Buffered Formalin

Question 8

Not recommended for viral specimens

Answer 8

Calcium Alginate

Question 9

Urine, stool, sputum, swabs, and viral specimens should be stored at what temperature?

Answer 9

Refrigerator Temp (4C)

Question 10

CSF should be stored at what temperature

Answer 10

Incubator Temp (35C)

Question 11

Specimens for anaerobic culture, sterile body fluid, genital specimen and swabs (ear and eye)
are held in what temperature?

Answer 11

Room Temp (22 - 25C)

Question 12

Serum should be kept at what temp

Answer 12

-20C

Question 13

Tissue specimens and Fecal specimens should be stored at what temp

Answer 13

-70C

Question 14

Specimens that should be Plated ASAP

Answer 14

• Body fluids
• Bone specimens
• Gastric Biopsy
• Suprapubic aspirate of urine
• Prostatic Specimens

Question 15

Specimen that can be stored indefinitely at room temp

Answer 15

Hair, Nail, or Skin scrapings

Question 16

Specimens that should be immediately stored at 35C

Answer 16

• Blood
• Bone marrow specimens
• Corneal scrapings

Question 17

Specimen that can be stored for 6hrs at 35C

Answer 17

CSF

Question 18

Specimen that can be stored for 72hrs at 4C

Answer 18

Rectal swab
Stool culture

Question 19

Maintains the integrity of the specimen from the time of specimen collection until
laboratory processing of the sample

Answer 19

Transport Medium

Question 20

Transport medium for stool pathogens

Answer 20

Cary Blair

Question 21

Transport medium for virus transport media

Answer 21

Stuart’s

Question 22

Transport medium for Respiratory specimen

Answer 22

Amies

Question 23

Transport medium for Neisseria

Answer 23

Transgrow / Cary Blair

Question 24

Transport medium for Streptococcus agalactiae

Answer 24

Cary Blair

Question 25

Level 1 or Critical / Invasive Specimens

Answer 25

- Amniotic Fluid - Blood - Brain - CSF - Serous Fluids

Question 26

Level 2 or Unpreserved Specimens

Answer 26

- Bone - Feces - Sputum - Tissue - Other Body Fluids

Question 27

Level 3 or Quantitation Required Specimens

Answer 27

- Catheter Tip - urine - Tissue

Question 28

Level 4 or Preserved Specimens

Answer 28

- Urine - Feces - Swabs in holding media

Question 29

Blood should be drawn when

Answer 29

Febrile Episode (30 - 45mins before fever spikes)

Question 30

Concentration of SPS should not exceed ______

Answer 30

0.025% w/v

Question 31

What should be added to prevent interference in blood

Answer 31

1% gelatin

Question 32

Signs of growth in blood

Answer 32

Hemolysis Gas bubbles Turbidity

Question 33

Ideal manner of collection for blood culture

Answer 33

Butterfly Infusion

Question 34

How is CSF collected

Answer 34

Lumbar Tap / Spinal Tap

Question 35

Purposes of the 4 containers for CSF

Answer 35

1st - ClinChem/Sero 2nd - Micorbio for culture 3rd - Clin Hema for cell count 4th - Additional tests for Micobio

Question 36

Specimen of choice for detecting GIT pathogens

Answer 36

Stool

Question 37

4 samples from Respiratory Tract

Answer 37

Throat Swab Nasal Swab Nasopharyngeal Swab Sputum

Question 38

How many morning specimens for sputum should be collected

Answer 38

3 (1hr intervals)

Question 39

When should Sputum be collected

Answer 39

Morning (more concentrated)

Question 40

Used to determine the cause of vaginitis, urethritis, cervicitis as well as childbirth infections

Answer 40

Genitourinary Specimens

Question 41

Often used to determine STIs

Answer 41

Genitourinary Specimens

Question 42

Most fastidious organisms

Answer 42

Genitourinary Specimens

Question 43

Culture Method for Genitourinary Specimens

Answer 43

Chocolate Agar Plate

Question 44

Transport medium for Genitourinary Specimens

Answer 44

Jembec / Gono Pak

Question 45

How many swab must be collected for Genitourinary Specimens

Answer 45

2 (1 for gram staining, 1 for culture)

Question 46

Results from animal or human bites, burns, ulcers and traumatic wounds

Answer 46

Exogenous wound infection

Question 47

Attribute to bacterial sources within the patient

Answer 47

Endogenous wound infection

Question 48

5 Types of Culture Media

Answer 48

- Nonselective - Selective - Differential - Enriched Media - Enrichment Broth

Question 49

Culture Media selections

Answer 49

Blood Agar Plate Chocolate Agar plate MacConkey Agar

Question 50

Incubation Temp

Answer 50

35 - 37C

Question 51

Routine Incubation Time

Answer 51

48 - 77hrs

Question 52

Incubation Time for Anaerobes and Broth

Answer 52

5 - 7 days

Question 53

Use of cotton swab may release what

Answer 53

Toxic Fatty Acids

Question 54

3 different swab equipment that can be used for specimen collection for Bacterial Culture

Answer 54

Calcium Alginate Dacron Rayon

Question 55

3 way to get urine sample

Answer 55

Midstream clean catch Catheterized Suprapubic aspirate