Cell Structure Flashcards
(176 cards)
it contains the ocular lens
What magnification does the eye piece have? Why?
Has 10x magnification because it contains ocular lens
What can you add/take away from a light microscope that is inserted in the eye piece?
Eye piece graticule can be inserted or taken out (ruler)
What is a turret on a light microscope?
-Contains multiple objective lenses- can be rotated to different lenses
-also known as the revolving nose piece
What is the purpose of the objective lenses on light microscope?
- can be clicked into place by rotating the turret
- contains 4 different lenses= 4 possibilities of magnification to view specimen with
Where is the specimen located on light microscope?
On stage- ensure slide fits in 90* angle
What is the purpose of the stage?
-Specimen is placed onto the stage
- Clips are spring loaded- easily be opened using a lever
What is a condenser in microscopes?
Converges the light rays together to make a beam of focused light
-to pass through aperture in stage
-and then the sample on the slide
What is the iris diaphragm
Underneath the stage above the condenser- size of aperture through which light can pass can be controlled by moving a lever
- by controlling amount of light which passes through slide the specimen can be illuminated but with enough contrast to see details
What is the upper control
Stage clips can be moved backwards+ forwards to position sample
What is the lower control
Stage clips can be moved from side to side to position sample
What is the function of the course focus knob
Bigger/ rough adjustments in height of stage- so sample appears in field of view
What is the function of the fine focus knob
Smaller adjustments- to fine focus the image and view different parts of the same e.g. if 3 dimensions you can focus on each layer
What is the light source in light microscope
Light bulb or mirror
What image does the light microscope form/ produce
2D coloured images
Explain how image is magnified in light microscope
Rays of light pass through sample to form an image—- image is magnified by passing through objective lens times by ocular lens= observer sees magnified image of sample
What is the total magnification in a light microscope
Objective lense times ocular lens equals total magnification
Define microscopic
Items that are too small to be seen with the naked human eye
Define macroscopic
Items that are large enough to be seen with the naked human eye
What is the purpose of microscopes
-Produces a magnified, of the object
-allows biologists to make more accurate descriptions of structures
-give comparisons with other structures
-so we can examine organelles in more details
Advantages of light microscope
-uses light rays to observe object
-can observe living organisms
-does not use harsh chemicals e.g. for staining
-easy to set up and use
-cheap and portable
-colored image
Disadvantages of Light microscope
-low magnification up to 2000 x
-low resolution due to long wavelength of light (200nm)
How to use a light microscope
1) Take a thin slice of specimen- use tweezers to place it in middle of a clean slide
2) Stain the specimen (e.g. eosin to see cytoplasm of cell)
3) Place a cover slip on top of specimen using a mounted needle at a 90* angle
4) Use the arms to clip the slide onto the stage and position so that it sits over the hole
5)Rotate the turret so that lowest objective lens power is in place
6) Use course focus knob to adjust the stage so that it sits underneath the objective lens and look through eye piece to ensure image is roughly in focus
7) Turn fine focus knob to adjust the focus of image until a clear image can be viewed from eye piece lens
8) Rotate the objective lens and select 10 x power and focus image into place using the course and fine adjustment knobs
Define Magnification
refers to how many times larger the image is compared to the object
Define resolution
minimum distance between two objects in which they can still be viewed as separate
-higher resolution= more detail can be distinguished