cell structure Flashcards
(102 cards)
the cell theory states that
- all living organisms are composed of one or more cells.
- cells are the basic structural and functional units in living things.
- all cells arise from pre-existing cells.
living cells typically have:
- membranes
- organelles
- hereditary information (DNA)
units of measurement
1 centimetre (cm) = 10^-2 m
1 millimetre (mm) = 10^-3 m
1 micrometer (µm) = 10^-3 mm = 10^-6 m
1 nanometer (nm) = 10^-3 µm = 10^-6 mm = 10^-9 m
1 angstrom (Å) = 10^-10 m
what is the difference between a eukaryote and a prokaryote
eukaryotes have a true nucleus and other membrane bound organelles, while prokaryotes do not
functions of nucleus
site of DNA replication and also the site of RNA synthesis via transcription
structure of nucleus
(or what the nucleus contains/is made up of)
- nuclear envelope
- nucleoplasm
- chromatin
- nucleolus
describe the nuclear envelope and its function(s)
composed of two membranes (an outer membrane and an inner membrane) separated by a fluid-filled space of 25 nm.
the outer membrane of the nuclear envelope is continuous with another organelle, the endoplasmic reticulum.
the nuclear envelope is perforated by nuclear pores. these pores are formed by large protein complexes known as the nuclear pores complexes (NPCs), which permit the passage of large molecules such as RNA (mRNA, tRNA), proteins and ribosomal subunits.
describe the nucleoplasm and its function(s)
the gel-like matrix of the nucleus.
contain substances such as chromatin, enzymes, chemical substances (ions, proteins / enzymes) and nucleotides.
it serves as a medium for diffusion of metabolites and large macromolecules
describe chromatin and its function(s)
DNA is organised along with histone proteins into chromatin.
double stranded DNA coils around histone octamer to form nucleosomes, with linker DNA between each nucleosomes. this results in chromatin described as beads-on-a-string.
during cell division, the chromatin coiled up into a highly condensed form and becomes visible as chromosomes.
what two varieties is chromatin found in?
euchromatin (transcriptionally active) and heterochromatin (transcriptionally inactive)
describe euchromatin
loosely coiled form of chromatin that is rich in gene concentration.
how does the structure of euchromatin make it transcriptionally active?
loosely coiled structure allows RNA polymerase and transcription factors to bind, initiating transcription.
describe heterochromatin
modifications to DNA and histone proteins results in a more tightly coiled chromatin structure.
what regions are usually heterochromatic?
regions that are non-coding (e.g. centromere, telomere) or contain genes that are transcriptionally inactive (due to spatial and temporal regulations)
describe the nucleolus
observed as one or more, large, dense and roughly spherical bodies in the nucleus of non-dividing cells, not surrounded by a membrane.
functions of the nucleolus
site of rRNA synthesis and assembly of ribosomal subunits
describe the structure of the smooth ER
- appears smooth due to lack of ribosomes on its surface
- contains a meshwork of fine tubules
- continuous with the outer membrane of the nuclear envelope
functions of the smooth ER
site of lipid synthesis (e.g. phospholipids, steroids, cholesterol)
storage and release of Ca^2+
(idk if below need remember)
in the adrenal gland, sER produces steroid hormones.
detoxification of drugs and poisons in the liver. SER may contain enzymes that catalyse reactions for detoxifying harmful drugs, alcohol, barbiturates (sedatives) and waste metabolic products.
sER of some stomach cells secrete hydrochloride acid, necessary for protein digestion.
structure of rough ER
- appears “rough” under electron microscope because of the presence of ribosomes on its surface
- a system of flattened membrane-bound sacs called cisternae
- continuous with the outer membrane of the nuclear envelope
function of rough ER
site of proteins synthesis where polypeptides fold into its native 3d conformation after being synthesised at the bound ribosomes
describe the golgi apparatus
consists of a stack of smooth surfaced, flattened membrane-bound sacs called cisternae and a system of associated vesicles called Golgi vesicles
state the functions of the golgi apparatus
- site of proteins and lipids chemical modification, e.g. glycosylation
- sorts and packages proteins into vesicles and targets them to various cellular locations.
describe the functions of the golgi apparatus
the polypeptides in ER are packaged into transport vesicles, which travel along microtubule tracks, to fuse with Golgi apparatus at the cis face (forming face).
In the GA’s cisternae space, the proteins and lipids undergo final chemical modification, e.g. glycosylation.
At the trans face (maturing face), vesicles pinches off from the GA forming Golgi vesicles.
what are the two types of golgi vesicles?
lysosomes: vesicles containing hydrolytic enzymes
secretory vesicles: vesicles containing proteins bound for secretion or proteins that are embedded on the cell surface membrane