cell structure Flashcards

(102 cards)

1
Q

the cell theory states that

A
  1. all living organisms are composed of one or more cells.
  2. cells are the basic structural and functional units in living things.
  3. all cells arise from pre-existing cells.
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2
Q

living cells typically have:

A
  • membranes
  • organelles
  • hereditary information (DNA)
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3
Q

units of measurement

A

1 centimetre (cm) = 10^-2 m
1 millimetre (mm) = 10^-3 m
1 micrometer (µm) = 10^-3 mm = 10^-6 m
1 nanometer (nm) = 10^-3 µm = 10^-6 mm = 10^-9 m
1 angstrom (Å) = 10^-10 m

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4
Q

what is the difference between a eukaryote and a prokaryote

A

eukaryotes have a true nucleus and other membrane bound organelles, while prokaryotes do not

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5
Q

functions of nucleus

A

site of DNA replication and also the site of RNA synthesis via transcription

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6
Q

structure of nucleus
(or what the nucleus contains/is made up of)

A
  1. nuclear envelope
  2. nucleoplasm
  3. chromatin
  4. nucleolus
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7
Q

describe the nuclear envelope and its function(s)

A

composed of two membranes (an outer membrane and an inner membrane) separated by a fluid-filled space of 25 nm.

the outer membrane of the nuclear envelope is continuous with another organelle, the endoplasmic reticulum.

the nuclear envelope is perforated by nuclear pores. these pores are formed by large protein complexes known as the nuclear pores complexes (NPCs), which permit the passage of large molecules such as RNA (mRNA, tRNA), proteins and ribosomal subunits.

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8
Q

describe the nucleoplasm and its function(s)

A

the gel-like matrix of the nucleus.

contain substances such as chromatin, enzymes, chemical substances (ions, proteins / enzymes) and nucleotides.

it serves as a medium for diffusion of metabolites and large macromolecules

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9
Q

describe chromatin and its function(s)

A

DNA is organised along with histone proteins into chromatin.

double stranded DNA coils around histone octamer to form nucleosomes, with linker DNA between each nucleosomes. this results in chromatin described as beads-on-a-string.

during cell division, the chromatin coiled up into a highly condensed form and becomes visible as chromosomes.

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10
Q

what two varieties is chromatin found in?

A

euchromatin (transcriptionally active) and heterochromatin (transcriptionally inactive)

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11
Q

describe euchromatin

A

loosely coiled form of chromatin that is rich in gene concentration.

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12
Q

how does the structure of euchromatin make it transcriptionally active?

A

loosely coiled structure allows RNA polymerase and transcription factors to bind, initiating transcription.

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13
Q

describe heterochromatin

A

modifications to DNA and histone proteins results in a more tightly coiled chromatin structure.

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14
Q

what regions are usually heterochromatic?

A

regions that are non-coding (e.g. centromere, telomere) or contain genes that are transcriptionally inactive (due to spatial and temporal regulations)

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15
Q

describe the nucleolus

A

observed as one or more, large, dense and roughly spherical bodies in the nucleus of non-dividing cells, not surrounded by a membrane.

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16
Q

functions of the nucleolus

A

site of rRNA synthesis and assembly of ribosomal subunits

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17
Q

describe the structure of the smooth ER

A
  • appears smooth due to lack of ribosomes on its surface
  • contains a meshwork of fine tubules
  • continuous with the outer membrane of the nuclear envelope
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18
Q

functions of the smooth ER

A

site of lipid synthesis (e.g. phospholipids, steroids, cholesterol)
storage and release of Ca^2+
(idk if below need remember)
in the adrenal gland, sER produces steroid hormones.
detoxification of drugs and poisons in the liver. SER may contain enzymes that catalyse reactions for detoxifying harmful drugs, alcohol, barbiturates (sedatives) and waste metabolic products.
sER of some stomach cells secrete hydrochloride acid, necessary for protein digestion.

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19
Q

structure of rough ER

A
  • appears “rough” under electron microscope because of the presence of ribosomes on its surface
  • a system of flattened membrane-bound sacs called cisternae
  • continuous with the outer membrane of the nuclear envelope
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20
Q

function of rough ER

A

site of proteins synthesis where polypeptides fold into its native 3d conformation after being synthesised at the bound ribosomes

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21
Q

describe the golgi apparatus

A

consists of a stack of smooth surfaced, flattened membrane-bound sacs called cisternae and a system of associated vesicles called Golgi vesicles

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22
Q

state the functions of the golgi apparatus

A
  1. site of proteins and lipids chemical modification, e.g. glycosylation
  2. sorts and packages proteins into vesicles and targets them to various cellular locations.
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23
Q

describe the functions of the golgi apparatus

A

the polypeptides in ER are packaged into transport vesicles, which travel along microtubule tracks, to fuse with Golgi apparatus at the cis face (forming face).

In the GA’s cisternae space, the proteins and lipids undergo final chemical modification, e.g. glycosylation.

At the trans face (maturing face), vesicles pinches off from the GA forming Golgi vesicles.

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24
Q

what are the two types of golgi vesicles?

A

lysosomes: vesicles containing hydrolytic enzymes

secretory vesicles: vesicles containing proteins bound for secretion or proteins that are embedded on the cell surface membrane

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25
the endomembrane system consists of
the nuclear envelope, rough and smooth endoplasmic reticulum (ER), the Golgi apparatus as well as the cell's plasma membrane, and includes the vesicles that bud off these membranes for intracellular transport exocytosis and endocytosis.
26
describe the structure of lysosomes
small, spherical vesicles that is surrounded by a single membrane. appear as darkly staining spherical bodies under electron microscope. contain hydrolytic enzymes such as lipases, proteases and nucleases. contents of lysosomes are acidic as the enzymes have a low optimum pH of 5.
27
functions of lysosomes
1. digestion of pathogens / materials ingested by phagocytosis / endocytosis 2. degradation of worn-out organelles during autophagy 3. autolysis 4. for the breakdown of extracellular content
28
describe the digestion of pathogens / materials ingested by endocytosis
after the cell carries out phagocytosis / endocytosis, the pathogen / materials are contained within a phagosome / endosome. fusion of the lysosome with the phagosme / endosome results in the formation of secondary lysosomes where ingested materials are digested.
29
phagocytosis vs endocytosis
(dont need exact wording just need understanding) Endocytosis is a general term for the cellular process in which substances are brought into the cell by engulfing them in a vesicle formed from the plasma membrane. Phagocytosis is a specific type of endocytosis, and the two terms are related but not interchangeable
30
describe the degradation of worn-out organelles during autophagy
worn-out organelles within a cell are fused with lysosomes, forming autophagosomes. enzymes in the lysosomes break down the organelles. the soluble products are absorbed into the surrounding cytoplasm where they may be used in the construction of new organelles.
31
describe autolysis
upon injury or infection, hydrolytic enzymes in lysosomes are released, leading to self-destruction of the cell.
32
state an example of lysosomes for the breakdown of extracellular content
the sperm releases its hydrolytic enzymes by exocytosis to digest the sheath of nutrient cells surrounding the ovum, to facilitate fertilisation
33
describe the structure of mitochondria (as well as how it relates to functions for some)
appears under EM mostly as rod-shaped or cylindrical (but may be dumbbell or spherical) vary in size, diameter within the range 0.5-1.5μm and length about 2.5-10μm consists of a double membrane separated by an extremely narrow fluid-filled space called intermembrane space. Smooth outer membrane: Highly permeable to small solutes but blocks passage of proteins and other macromolecules Highly convoluted inner membrane which forms cristae (singular: crista): Irregular series of partitions to increase the surface area for enzyme (ATP synthase) and protein (of electron transport chain) attachment needed for cellular respiration (oxidative phosphorylation) Intermembrane space: Temporary storage of protons to generate a proton gradient for electron transport chain The membranes enclose the mitochondrial matrix which is semi-fluid and finely granular. It is the site of Krebs cycle during aerobic respiration and site of fatty acid oxidation. The matrix contains circular mitochondrial DNA (mtDNA) and 70S ribosomes, which are characteristic of prokaryotes (refer to B. Prokaryotes). These enable mitochondria to be semi-autonomous whereby they can grow and divide independently of the cel
34
function of mitochondria
site of aerobic respiration for ATP synthesis
35
how do chloroplasts try to absorb as much light as possible?
by moving through the cell by cytoplasmic streaming
36
structure of chloroplast (and how it relates to functions for some)
Biconvex discs / lens-shaped Enclosed by a double membrane collectively known as the chloroplast envelope The outer and inner membranes are separated by a very narrow fluid-filled intermembrane space. Outer membrane – This is a smooth, permeable membrane that allows small molecules and ions to pass freely. However, larger molecules require transport proteins. Inner membrane – This membrane is less permeable and contains specific transport proteins that regulate the movement of substances such as metabolites, ions, and proteins into and out of the chloroplast. Thylakoid membrane are found in the stroma, inside the chloroplasts. It is a system of flattened membranous sacs where the light-dependent reactions of photosynthesis occur, including the processes involving photosystems I and II, electron transport chain, and ATP synthesis. Thylakoids form stacks known as grana (singular: granum), and the thylakoid connecting the grana is known as the inter-granal thylakoid / lamellae. The fluid surrounding the thylakoid membrane is the stroma, which contains the chloroplast circular DNA, 70S ribosomes, starch grains and enzymes.
37
function of chloroplast
site of photosynthesis, where light-dependent reactions of photosynthesis occur on the thylakoid membrane and light-independent reactions occur in the stroma
38
structure of plant vacuoles
filled with cell sap, an aqueous solution of dissolved food materials, ions, waste products and pigments. enclosed by single membrane known as tonoplast.
39
functions of plant vacuoles
1. Stores reserves of important organic compounds (eg. protein storage in seeds) 2. Stores inorganic ions. e.g. K+ and Cl- 3. Disposal sites for metabolic by-products e.g. calcium oxalate crystals / latex, that would endanger the cell if they accumulated in the cytosol 4. May contain pigments that colour the cells. e.g red and blue pigments of petals that help attract pollinating insects to flowers, red onion. 5. May help protect the plant against predators by containing compounds that are poisonous or unpalatable to animals such as alkaloids. 6. Plays a role in plant growth by absorbing water and elongating the cell
40
structure of animal vacuoles
usually very much smaller and less permanent than plant vacuole small vacuoles are often called vesicles and may contain engulfed solids or liquids
41
functions of animal vacuoles
1. Food vacuoles: Formed by phagocytosis, e.g. in the case of intracellular digestion by some protozoa and macrophages 2. Contractile vacuoles: Found in many freshwater protists to pump excess water from the cell (osmoregulation) e.g. Paramecium had contractile vacuoles that pump excess water out of the cell.
42
list the membrane bound organelles
nucleus, endoplasmic reticulum, Golgi apparatus, lysosome, mitochondria, chloroplast, vacuoles
43
list the non-membrane bound organelles
ribosomes, centrioles, cytosol, cytoskeleton, cellulose cell wall
44
what two types of ribosomes are there?
1. free ribosomes, which are suspended in the cytosol 2. bound ribosomes, which are attached to rough endoplasmic reticulum
45
structure of ribosomes
Consists of 2 subunits – a small subunit and a large subunit. Ribosomes are composed of ribosomal proteins and rRNA. rRNA is synthesized in the nucleolus of eukaryotic cells. This is also the site where ribosomal RNA and proteins assemble into ribosomal subunits.
46
function of ribosomes
site of polypeptide synthesis during translation
47
in eukaryotes, where are proteins that functions within the cytosol synthesised?
at the free ribosomes
48
in eukaryotes, where are the proteins that are membrane-bound, enclosed within membrane or destined for secretion synthesised?
at the bound ribosomes on the rough ER
49
describe the synthesis of ribosomes in eukaryotes
rRNA genes transcribed to give rRNA in the nucleolus. Ribosomal protein genes are transcribed in the nucleus to give mRNA in the nucleus. mRNA is translated at free ribosomes in the cytoplasm. Ribosomal proteins are transported into the nucleolus via nuclear pores. Ribosomal proteins and rRNA assemble in the nucleolus to form separate small and large subunits. The subunits assemble on mRNA during translation.
50
where are centrioles found?
Found in animal cells and lower plant cells (e.g. algae and mosses) but absent in higher plant cells. Located next to the nucleus. Found within a region known as the centrosome during nuclear division.
51
structure of centrioles
Exist as a pair of rod-like structures, positioned with their longitudinal axis at right angles to each other Cylinder is made up of 9 triplets of microtubules arranged in a ring.
52
function of centrioles
organise spindle fibres during cell division of animal cells
53
what does the cytosol contain
contains water (90%), organic molecules, inorganic ions and waste products (CO2) as well as many enzymes.
54
what is the function of the cytosol
Store vital chemicals and site of certain metabolic pathways e.g. glycolysis, fatty acid synthesis, translation
55
what does the cytoskeleton consist of?
microtubules, intermediate fibres, and microfilamets
56
function of cytoskeleton
maintain cell shape, anchor organelles, and cause cell movement
57
structure of microtubules
made of globular protein tubulin (α-tubulin and β-tubulin) tubulin tube may elongate by adding tubulin units to one end straight, hollow fibres about 25nm in diameter may occur singly or in bundles may dissociate or reassemble to build microtubules elsewhere in the cell
58
structure of microfilaments
made of globular protein actin solid structures (not tubular) with diameter about 5-7nm which appears as a helix of two intertwining actin chains may occur as bundles in the cytoplasm may dissociate or reassemble to build microfilaments elsewhere in the cell
59
structure of intermediate fibres
made of diverse proteins within the keratin family solid structure with diameter of about 10nm may occur as bundles in the cytoplasm more stable than microtubules and microfilaments to enforce cells and organise tissue
60
functions of microtubules
cellular support tracks for organelle movement (involves protein known as kinesin and dyenin) cellular movement -- formation of cilia and flagella for motility separation of chromosomes during nuclear division deposition of cellulose
61
function of microfilaments
cellular support localised contraction of cells, such as the pinching of cell into two during cell division muscle contraction cytoplasmic streaming - important in moving organelles such as mitochondria and chloroplast
62
function of intermediate fibres
structural role: provides mechanical strength to cells and tissues fixes the location of certain organelles e.g. nucleus sits within a cage of intermediate filaments
63
structure of cellulose cell wall
External to plasma membrane Consists of cellulose fibres embedded in an amorphous polysaccharide matrix of pectin/lignin Permeable to water and solutes
64
function of cell wall
To provide mechanical support for plant cell and to the plant (especially herbaceous plants) As water enters the cell osmotically, the cell wall resists expansion and an internal pressure is created which provides turgidity for the cell and the plant. The strength may be increased by the presence of lignin in the matrix between the cellulose fibres.
65
what do prokaryotic cells ALWAYS contain
peptidoglycan cell wall, plasma membrane, genetic material, 70S ribosomes
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what do prokaryotic cells contain NOT ALL THE TIME
plasmids, capsule, flagella, pili
67
structure of petidoglycan cell wall
Peptidoglycan consists of parallel polysaccharide chains made of alternating units of N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), cross-linked in regular fashion by short peptide chains. Bacteria can be classified as gram positive or gram negative based on the structure of their cell walls.
68
gram positive bacteria vs gram negative bacteria
gram positive bacteria: - Have a thick layer of peptidoglycan and no outer membrane - Stained blue-purple by gram stain gram negative bacteria: - Have a thin layer of peptidoglycan and an outer membrane / envelope. - Stained red by gram stain
69
functions of peptidoglycan cell wall
confers the cell rigidity and shape prevents the cell from lysis when it absorbs water via osmosis
70
plasma membrane structures and functions
similar to that of the eukaryotic cells
71
other membranal structures in prokaryotic cells
mesosome (only in some bacteria) and photosynthetic membranes (only in photosynthetic bacteria)
72
what are mesosomes
infoldings of the cell membrane which are formed in some bacteria during binary fission
73
functions of mesosomes
- facilitate the separation of the two daughter molecules of DNA after replication. - increase the surface area for enzymatic reactions that are associated with respiration.
74
what do photosynthetic membranes contain
photosynthetic pigments e.g. chlorophyll
75
describe the genetic material in prokaryotic cells/bacteria
Bacteria have only 1 chromosome, which is a single circular double-stranded DNA containing only several thousand genes. Found in nucleoid region (not membrane bound). Associated with histone-like proteins Transcriptionally active genes are found in loosely coiled regions Transcriptionally inactive genes found in supercoiled regions
76
what are plasmids
Are small, self-replicating circular DNA (contains its own origin of replication) occurring in addition to the chromosomal DNA They possesses only a few genes, which generally give extra survival advantage
77
give examples of how plasmids possess genes that give extra survival advantage
E.g. genes which confer resistance to antibiotics E.g. F plasmid contains fertility factor gene which confers sex pili
78
structure of capsules
Is a slimy secretions of certain bacteria. Usually consist of polysaccharides.
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functions of capsules
Can serve to unite bacteria into colonies. Also enable bacteria to stick to surfaces such as teeth, mud and rocks, and offer useful additional protection to the bacteria.
80
structure of flagella
rigid and wave-shaped
81
functions of flagella and how it functions
many bacteria are motile due to one or more flagella cork screw motion of flagella facilitates movement of bacteria
82
structure of pili
Are surface appendages / fine protein rods projecting from the walls of some bacteria. Shorter and thinner than flagella
83
function of pili
aid in attachment to specific cells or surfaces
84
example of function of pili
Sex pili are involved in bridging two bacteria, thus allowing the donor bacteria to transfer DNA to the recipient bacteria. This results in conjugation, a form of horizontal gene transfer.
85
what does a virion consist of
1. viral genome 2. capsid protein 3. envelope (present only in some viruses)
86
what is a genome / structure of genome
The viral genome is a molecule of nucleic acid (either DNA or RNA) that functions as the genetic material of the virus. It can be: - single or segmented - circular or linear - single-stranded or double-stranded The genes present on the viral genome are relatively few in number, ranging from 3-1000 depending on species.
87
function of viral genome
codes for the synthesis of the viral components and viral enzymes required for replication.
88
what is a capsid / structure of capsid
The capsid is the protective protein coat enclosing the viral genome. Together, the structure is known as nucleocapsid. Capsids are formed from structural subunits called capsomeres, arranged in a precise and highly repetitive pattern around the nucleic acid. The information for proper assembly of proteins into capsomeres is contained within the structure of the proteins themselves, and the overall process of assembly is thus called self-assembly.
89
function of capsid
The capsid serves to protect and introduce the genome into the host cells.
90
what are naked viruses?
viruses without envelope
91
what are enveloped viruses?
viruses that have complex membranous structures surrounding the polyhedral or helical nucleocapsid
92
what are envelopes composed of
phospholipids and glycoproteins (for most viruses)
93
how are envelopes derived
they are derived from host cell membranes by a process called budding. The envelope comes from the host cell’s nuclear membrane, ER, vacuolar membranes, or cell surface membrane. Although the envelope is usually of host origin, the virus does incorporate proteins of its own, often appearing as glycoprotein spikes, into the envelope.
94
what are the functions of glycoprotein spikes
the glycoprotein spikes function in attaching the virus to receptors on susceptible host cells (recognition).
95
what are bacteriophages?
viruses that only infect bacteria
96
typical structure of bacteriophage
1. Genome: dsDNA 2. Capsid: Capsomeres, Sheath, Base plate, Tail fibres 3. No envelope / Naked
97
what are viruses?
obligate intracellular parasites
98
why are viruses obligate intracellular parasites?
because they must invade a cell (host) to replicate and multiply. Viruses require the host’s machinery, e.g. ribosomes and enzymes, to synthesize their own genetic materials and proteins.
99
arguments for viruses being non-living
1. Lack of Cellular Structure: Viruses are not made of cells, which are considered the basic units of life according to cell theory. 2. No Metabolism: They do not carry out metabolic processes on their own, such as energy production or nutrient assimilation. 3. Dependence on Host: Viruses cannot reproduce independently. They need a host cell to replicate, which suggests they cannot sustain life processes autonomously. 4. No Response to Stimuli: Viruses generally do not respond to environmental stimuli in the way living organisms do.
100
arguments for viruses being living
1. Genetic Material: Viruses contain genetic material (DNA or RNA), which they use to direct the production of new virus particles within a host cell. 2. Evolution: They can evolve over time through mutations and natural selection, a characteristic of living organisms. 3. Reproduction: While they rely on a host, they do reproduce, producing numerous copies of themselves once inside the host cell.
101
are viruses considered cells?
according to cell theory, viruses are not considered cells.
102
why aren't viruses considered cells (according to cell theory?)
1. Living organisms are composed of one or more cells: Viruses are not made up of cells. They lack the cellular structure found in living organisms, such as a plasma membrane, cytoplasm, and organelles. 2. The cell is the basic unit of structure and function in living things: Viruses do not have the structural components or functional independence of cells. They cannot carry out metabolic processes or reproduce on their own; they require a host cell to replicate. 3. All cells arise from pre-existing cells: Viruses do not come from other cells. Instead, they replicate by assembling new virus particles inside a host cell, which is a different mechanism from cellular division (e.g., mitosis or binary fission).