Cells calculation toolkit Flashcards
(12 cards)
1
Q
General formula for concentration
A
c = n or m / v
2
Q
Concentration changes (dilutions)
A
c1v1 = c2v2
3
Q
amount changes (dilutions)
A
n1/v1 = n2/v2
4
Q
volume of a sphere
A
(4πr^3) / 3
5
Q
Beer-Lambert Law
A
A = cƐl
where c = conc. (mole/l)
Ɛ = molar extinction coefficient (l/mole/cm)
l = path length (cm)
6
Q
How find out concentration by absorbance?
A
- dilution series of known conc.
- run absorbance assays
- draw calibration curve
- record absorbance of unknown solution, use curve.
7
Q
Formula for moles
A
moles = mass/mr
8
Q
Moles molecules
A
moles = molecules/avogadro’s no.
9
Q
Formula for catalytic activity
A
A = amount/time
A = katal (K) or enzyme unit (U)
amount can be moles or mass
10
Q
Michaelis-Menten equation
A
Vo = Vmax ([S]/[S]Km)
11
Q
mV Oxygen conc. (from oxygen electrode)
A
1000mV = air saturated water = 0.255μmole O2/ml
at 20°C sol. of oxygen in water = 0.255μmole O2/ml
12
Q
1 litre –> decimetres cubed
A
1L = 1dm^3
