Cellular and Sub cellular Components

Question 1

Color of top that has NO FIBRINOGEN

What does it chelate in the blood?

Answer 1

EDTA (Violet/lavander top)

Chelates Calcium

Question 2

A non additive top that forms clot

What cellular component does it produce?

Answer 2

Red top

SERUM

Question 3

What kind of sample has Heparin?

Answer 3

Capillary sample

Question 4

What are the therapeutic blood components?

IN ORDER

Answer 4

Whole blood
Packed RBC
Fresh Frozen Plasma
PH concentrate
Cryoprecipitate
Cryosupernate

Question 5

What can we get in whole blood?

Answer 5

Plasma
WBC
RBC
Platelets

Question 6

What blood components are reduced in Anemic patients? *what can be the cause?

Answer 6

Hemoglobin (Hgb)
Hematocrit? (Hct)
*Active bleeding

Question 7

In fresh frozen plasma, what is the factor that can cause active bleeding?

Answer 7

REDUCED Factor VIII

Question 8

It is the only blood component stored at room temperature? *What is the shelf life?

Answer 8

PH concentrate

*shorter shelf life

Question 9

ORDER OF DRAW and what does each top contain?

Answer 9

YELLOW - Sodium Polyatenol Sulfonate (SPS)
BLUE - Na (sodium) Citrate
RED - non additive
GREEN - Heparin
Purple - EDTA
Gray - NaFl (sodium flouride) enoulase?

Question 10

Top used for blood culture

Answer 10

YELLOW

Question 11

Top used for coagulation studies

Answer 11

BLUE

Question 12

RED top is used for what tests?

Answer 12

Blood chem and serological testing

Question 13

GREEN top is used for what tests?

Answer 13

Blood gas analysis and Acute Myocadial Infartion (Troponin test)

Question 14

Top used for sugar level

Answer 14

GRAY

Question 15

In CBC, what Leukocytes are HIGHER in bacterial infection?

Answer 15

Neutrophils and Monocytes

Question 16

In CBC, what Leukocytes are HIGHER in allergic reaction and PARASITIC INFECTION?

Answer 16

Eosinophil

Question 17

HIGHER count of Basophil in CBC, indicates?

Answer 17

Allergic reaction

Question 18

LOWER count of ________ in CBC, indicates VIRAL INFECTION

Answer 18

Lymphocytes

Question 19

In CBC, what Leukocytes are HIGHER in ACTIVE TB or bacterial infection?

Answer 19

Monocytes

Question 20

CBC range of Hemoglobin

Answer 20

120 g/L to 145 g/L

Question 21

CBC range of Hematocrit

Answer 21

35 (L/L?) to 48 (L/L)

Question 22

CBC range of RBC

Answer 22

5-10k per liter

Question 23

Differential count of WBC. HIGHEST TO LOWEST.

Answer 23

NLMEB (Never Let Monkey Eat Banana)
Neutrophil
Lymphocytes
Monocytes
Eosinophil
Basophil

Question 24

Platelet ranges in CBC

Answer 24

150-450x10^3 /L

Question 25

Isolation of organelles in their relatively pure form and analysis of macromolecules

Answer 25

Sub cellular Fractionation

Question 26

3 general procedures of sub cellular fractionation

Answer 26

Extraction Homogenization Centrifugation

Question 27

Process of isolating organelles using 0.025M *_____ solution.

Answer 27

Extraction | *Sucrose

Question 28

In extraction, Temperature must be?

Answer 28

LESS THAN 4 degrees celcius

Question 29

Why sucrose solution or NSS solution is used in extraction?

Answer 29

It does not cross the cell membrane and does not cause the organelles to swell

Question 30

process where cells are opened in an isotonic buffer

Answer 30

Homogenization

Question 31

Homogenization is achieved by?

Answer 31

Sonication Detergent French press Mechanical homogenization

Question 32

High frequency soundwaves

Answer 32

Sonication

Question 33

used for lysis

Answer 33

Detergent

Question 34

for high pressure

Answer 34

French press

Question 35

Blender

Answer 35

Mechanical homogenization

Question 36

procedure that is repeated and progressively higher speeds will fractionate

Answer 36

Centrifugation

Question 37

What are the fractions we can get in Sub cellular Fractionation? *INDICATE TIME

Answer 37

Nuclear - 10 mins Mitochondiral - 20 mins Microsomal - 40 - 60 mins

Question 38

Fraction that has nucleic acids, DNA, and RNA

Answer 38

Nuclear fraction

Question 39

Mitochonrial fraction has?

Answer 39

Mitochondria (predominantly carbs) | Lysosomes and golgi bodies (can be isolated)

Question 40

Microsomal fraction has?

Answer 40

Microsomes, ribosomes, cytoplasm and endoplasmic reticulum

Question 41

Different tests in qualitative analysis and what can they identify?

Answer 41

``` Biuret test - proteins Sudan IV - lipids Molisch test - carbohydrates Dische test - DNA Orcinol test - RNA ```

Question 42

What is the reaction medium in Biuret test?

Answer 42

NaOH

Question 43

What is the oxidizing agent in Biuret test?

Answer 43

CuSO4

Question 44

Positive color for Biuret

Answer 44

Violet or purple

Question 45

uses hydrophobic dye for _____ and what is the positive color?

Answer 45

Sudan IV for Lipids. Orange to red

Question 46

What is the ability of Sudan IV?

Answer 46

Absorb colors in fats

Question 47

test for carbs. | What can be seen in RAPID and SLOW?

Answer 47

Molisch Rapid: Monosaccharides Slow: Disaccharides and Polysaccharides

Question 48

what reagents are combined for Molish?

Answer 48

Alpha-napthol in alcohol + concentrated H2SO4

Question 49

test for 2-deoxypentose* | What color of formation?

Answer 49

Dische. BLUE. | *DNA

Question 50

test for pentose* | What color?

Answer 50

Orcinol/Bial's *5 carbon sugar BLUE-GREEN

Question 51

nucleic acid in nuclear fraction

Answer 51

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Question 52

Protein in nuclear fraction

Answer 52

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Question 53

Lipids in nuclear fraction

Answer 53

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Question 54

Carbs in nuclear fraction

Answer 54

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Question 55

nucleic acid in mitochondrial fraction

Answer 55

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Question 56

Protein in mitochondrial fraction

Answer 56

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Question 57

Lipids in mitochondrial fraction

Answer 57

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Question 58

Carbs in mitochondrial fraction

Answer 58

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Question 59

nucleic acid in microsomal fraction

Answer 59

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Question 60

Protein in microsomal fraction

Answer 60

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Question 61

Lipids in microsomal fraction

Answer 61

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Question 62

Carbs in microsomal fraction

Answer 62

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