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SLMC INTERNSHIP > Cellular Immunology Part 1 > Flashcards

Cellular Immunology Part 1 Flashcards

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1
Q

The field concerning the interactions among cells and molecules of the immune system, and how such interactions contribute to the recognition and elimination of pathogens

A

Cellular Immunology

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2
Q

The passage of cells in single file in front of a laser so they can be detected, counted, and sorted.

A

Flow Cytometry

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3
Q

Cell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths detected by detectors

A

Flow Cytometry

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4
Q

Cell size

A

Forward Scatter

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5
Q

Cell granularity/lobes

A

Side Scatter

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6
Q

____________ collected by _____________ and _________ creates patterns that are characteristic of the size and internal complexity of the cell.

A

Light scatter collected by forward (FCS) and side scatter (SSC) creates patterns that are characteristic of the size and internal complexity of the cell.

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7
Q

CD is an abbreviation for

A

Cluster of Differentiation

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8
Q

cell surface markers which are very useful for the identification and characterization of leukocytes and the different subpopulations of leukocytes

A

CD molecules or CD markers

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9
Q

CD45

A

Backbone marker

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10
Q

Hematopoietic marker expressed on all leukocytes

A

CD45

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11
Q

CD3

A

Pan T Lymphocytes

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12
Q

CD4

A

T Helper cells

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13
Q

CD 8

A

T suppressor

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14
Q

Cytotoxic T cells

A

CD8

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15
Q

CD16/56

A

Natural Killer Cells

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16
Q

CD19

A

Pre B cells

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17
Q

CD20

A

Pan B cells

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18
Q

(6) Tests with Flow Cytometry as Principle

A
  1. FACS CD4 (Fluorescence Activated Cell Sorting)
  2. Primary Immunodeficiency Panel 1 (PID 1)
  3. Primary Immunodeficiency Panel 3 (PID 3)
  4. Flow PRA (Panel Reactive Antibody)
  5. Flow Cytometric Crossmatching
  6. PNH Panel (Paroxysmal Nocturnal Hemoglobinuria)
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19
Q

Used to enumerate the absolute counts of CD4 T lymphocytes

A

Fluorescence Activated Cell Sorting (FACS CD4)

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20
Q

FACS CD4 sample requirement?

A

1 EDTA
1 Sodium Heparin (4ml whole blood)

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21
Q

Markers of FACS CD4

A

CD3
CD4
CD8

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22
Q

CD4:CD8 Ratio

Normal:

A

2:1

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23
Q

CD4:CD8 Ratio

HIV+/Px with AIDS

A

1:2

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24
Q

Primary Immunodeficiency Panel 1 is also known as

A

Lymphocyte Subset Enumeration Test

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25
Sample requirement of PID 1
1 EDTA 1 Sodium Heparin (4ml whole blood)
26
CD Markers of PID 1
CD3 CD4 CD8 CD16/56 CD19 CD20
27
Primary Immunodeficiency Panel 3 is also known as
Lymphocyte Subset Enumeration Test
28
Sample requirement of PID 3
1 EDTA 1 Sodium Heparin 1 Red Top (4ml whole blood)
29
Test with Serology Test?
Primary Immunodeficiency Panel 3
30
PID 3 Serology Tests
IgG IgA IgM IgE Complement 3
31
Flow PRA
Panel Reactive Antibody
32
a routine screening measure to assess the degree of a potential kidney recipient's sensitization, as a result of prior exposure to external HLA antigens during previous blood transfusions, pregnancies, or organ transplantations
Flow Panel Reactive Antibody
33
Flow PRA is also known as
Lymphocyte Antibody Test
34
Sample requirement of PRA
2x 4ml Red top (Serum)
35
Result of PRA is via ___________
Percentage (%Qualitative: Positive or Negative with Percentage)
36
Flow PRA cutoff:
10%
37
Flow PRA is commonly requested for
Kidney Transplant Patients
38
True or False: In the test flow cytometric crossmatching both the donor and recipient must be tested
True
38
Flow Cytometric Crossmatching is also known as
T and B cell crossmatching
39
Time Sensitive Test
Flow Cytometric Crossmatching
40
Assess preformed antibodies in the recipient against donor's antigen (Flow Cytometry)
Flow Cytometric Crossmatching
41
Sample Requirement for Flow Cytometric Crossmatching
2 ACD Tubes 1 Red Top Each
42
Always ask if the recipient undergoes Hemodialysis. If yes, samples must be collected after ___-___hours post HD
24 - 48 hours
43
CD Markers of Flow Cytometric Crossmatching
CD3 (T cell) CD 22 IgG (B cell)
44
Detects the presence or absence of white blood cell (WBC) markers called antigens. These antigens are protein structures found on or within WBCs
Immunophenotyping
45
Accepted Samples Except PNH: Bone Marrow Aspirate: Peripheral Blood: Cerebrospinal Fluid: Other Bodily Fluids:
Bone Marrow Aspirate: 2-4mL Sodium Heparin Peripheral Blood: 2x 4mL Sodium Heparin Cerebrospinal Fluid: 3-5mL Other Bodily Fluids: (e.g. Pleural Fluid)
46
CD34 is a marker for
Marker for Blasts
47
Characterized by destruction of red blood cells, blood clots, and impaired bone marrow function
Paroxysmal Nocturnal Hemoglobinuria
48
PNH is a decreased or absent expression of ________ and/or _______
CD55 and/or CD59
49
Sample requirement for PNH Panel
2x 4mL EDTA
50
CD Markers tested in PNH Panel
CD45 CD55 CD59 FLAER
51
Paroxysmal Nocturnal Hemoglobinuria is also known as
Ham Test
52
CD55 also known as
DAF - Decay Accelerating Factor
53
CD59 is also known as
MIRL - Membrane Inhibitor of Reactive Lysis
54
(2) Machines used in Flow Cytometry
BDFacsLyric BDFacsCalibur
55
FLAER means
Fluorescent Aerolysin
56
(2) Tests for Complement Dependent Cytotoxicity (CDC)
1. Tissue Crossmatching 2. Human Leukocyte Antigen (HLA B27)
57
CDC means
Complement Dependent Cytotoxicity
58
Tissue Crossmatching is also known as
T Cell Crossmatching
59
True or False: In the test Tissue Crossmatching both the donor and recipient should not be tested
False
60
Assess preformed antibodies in the recipient against donor's antigen (CDC)
Tissue Crossmatching
61
Sample Requirement of Tissue Crossmatching
2 ACD Tubes 1 Red Top Each
62
(3) Disease associated with Human Leukocyte Antigen
Ankylosing Spondylitis Reactive Arthritis Uveitis
63
Sample Requirement for HLA B27
2 ACD Tubes
64
A. Recipient Serum (May contain Donor-specific HLA antibodies) + Donor Lymphocytes + Complement - No donor-specific HLA antibodies in recipient Serum; No Antibody binds Result:
Negative Crossmatch (No cell Lysis)
65
A. Recipient Serum (May contain Donor-specific HLA antibodies) + Donor Lymphocytes + Complement - Donor-specific HLA antibodies in recipient serum: Antibody binds Complement activated Result:
Positive Crossmatch (>20% of cells lysed)
66
HLA is also known as
MHC - Major Histocompatibility Complex
67
Location of HLA
Short arm of Chromosome 6
68
Proteins that help the body's immune system to tell difference between self and non-self
Human Leukocyte Antigen
69
A procedure in which the tissues of a prospective donor and recipient are tested for compatibility prior to transplantation.
HLA Typing
70
Mismatched donor and recipient tissues can lead to rejection of the tissues
HLA Typing
71
Test used to match patients and donors for bone marrow, cord blood, or organ transplants
HLA Typing
72
HLA Tissue Typing Class I and II Principle:
HLA Class I done by Serological Method. HLA Class II DNA typing by Micro SSP.
73
HLA Tissue Typing Class I and II Commonly for
Kidney Transplant
74
What method used in HLA Tissue Typing Class I and II
Serological Method
75
HLA Tissue Typing Class I and II Locus: HLA I: HLA II:
HLA I: A, B, C HLA II: DR, DQ
76
HLA Tissue Typing Class I and II Sample requirement:
2x ACD (10cc) 1x Red Top (10cc)
77
True or False. HLA Tissue Typing Class I and II performed blood typing
True
78
HLA Tissue Typing Class I and II Machine:
ABI 7500 Fast Real Time PCR
79
HLA DNA Typing with C and DP Principle:
RSSO - Reverse Sequence Specific Oligonucleotide
80
HLA DNA Typing with C and DP Commonly for:
Bone Marrow Transplant Kidney Transplant (Rarely)
81
HLA DNA Typing with C and DP Method used:
Molecular Method
82
HLA DNA Typing with C and DP Locus: HLA I: HLA II:
HLA I: A, B, C HLA II: DR, DQ, DP
83
HLA DNA Typing with C and DP Sample Requirement:
2x ACD (10cc) ONLY
84
True or False. HLA DNA Typing with C and DP performed blood typing
False
85
HLA DNA Typing with C and DP Machine:
LUMINEX LABScan 100

SLMC INTERNSHIP (2 decks)

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