Cellular Immunology Part 2 (Other Tests)

Question 1

Quantitative monitoring test for HBV/HIV/HCV

Answer 1

HBV/HIV/HCV (Viral Load)

Question 2

Sample Requirement for Viral Load

Answer 2

Plasma

Question 3

GeneXpert Instrument Systems automate and integrate sample purification, nucleic acid amplification, and detection of the target sequence using _______________ and ____________

Answer 3

Real-Time Reverse Transcriptase PCR (RT-PCR)

Real Time PCR

Question 3

Viral load is performed on ______

Answer 3

Cepheid GeneXpert Instrument Systems

Question 4

Human Papilloma Virus (HPV) High risk with ____, ____ Genotyping

Answer 4

16, 18/45 Genotyping

Question 5

Human Papilloma Virus (HPV)

Sample Requirement (Most common)

Answer 5

Cervical Sampler

Question 6

Cartridge based assay; advantage: Faster TAT

Answer 6

Human Papilloma Virus (HPV)

Question 6

Human Papilloma Virus (HPV)

Sample Requirement (Other samples accepted)

Answer 6

Tongue Mass
Laryngeal Mass

Question 7

BK Virus is also known as

Answer 7

Human Polyomavirus 1

Question 8

causes trivial symptoms in the immunocompetent, although it may cause significant pathology in the immunosuppressed transplant recipient (Kidney transplant)

Answer 8

BK Virus

Question 9

Is an abbreviation of the name of the first patient whom the virus was isolated from.

Answer 9

BK Virus

Question 9

Monitoring test together with CMV viral load

Answer 9

BK Virus

Question 10

Usually, primary infection has occurred during childhood then the virus could be latent through life, especially in the kidneys and urinary system

Answer 10

BK Virus

Question 11

BK Virus

Organ of Interest:

Answer 11

Kidneys

Question 11

Principle of BK Virus

Answer 11

Real Time PCR

Question 12

BK Virus

Specimen used:

Answer 12

Plasma
Serum
Urine

Question 13

T-SPOT TB TEST

Principle:

Answer 13

IGRA - Interferon Gamma Release Assay

Question 14

BK Virus

Machine used:

Answer 14

3M Integrated Cycler

Question 15

Is a blood test used to see whether a person has been infected with Mycobacterium tuberculosis (the bacteria causing TB.

Answer 15

IGRA - Interferon Gamma Release Assay

Question 16

Works by measuring the body’s immune response to the TB bacteria

Answer 16

IGRA

Question 17

May be used to diagnose latent TB infection

Answer 17

IGRA

Question 17

T-Spot TB Test

Specimen Requirement:

Answer 17

3x 4mL Lithium Heparin (freshly collected)

Question 18

T-Spot TB Test

TB-specific antigens:

Answer 18

ESAT-6 and CFP10

Question 19

To evaluate if the chromosome 1p or 19q has deletion or no deletion

Answer 19

FISH 1P19Q

Question 20

FISH 1P19Q Short arm of chromosome __, and long arm of chromosome ___.

Answer 20

Short arm - 1 Long arm - 19

Question 21

FISH 1P19Q Specimen used:

Answer 21

Tissue Block (Brain)

Question 22

FISH 1P19Q Result: <.80 Deletion (Astrocytoma) - >.80 Non-Deletion (Oligodendroglioma) -

Answer 22

<.80 Deletion (Astrocytoma) - Bad Prognosis >.80 Non-Deletion (Oligodendroglioma) - Good Prognosis

Question 23

STR DNA Analysis

Answer 23

Short Tandem Repeat

Question 24

STR DNA Analysis Principle:

Answer 24

Capillary Electrophoresis

Question 25

STR DNA Analysis separates molecules due to their electrophoretic mobilities

Answer 25

Capillary Electrophoresis

Question 26

A molecules electrophoretic mobility depends on its charge and how much it is attracted or repelled by the voltage as well as the frictional drag force that resists movement

Answer 26

STR DNA Analysis

Question 27

Reasons for DNA Testing:

Answer 27

Personal Legal Embassy

Question 27

Anode: Cathode:

Answer 27

Anode: (+) Cathode: (-)

Question 28

STR DNA Analysis Sample requirement:

Answer 28

Buccal Swabs

Question 29

Paternity/Maternity testing

Answer 29

STR DNA Analysis

Question 30

STR DNA Analysis Machine:

Answer 30

ABI 3500 Genetic Analyzer

Question 31

STR Engraftment Analysis/Chimerism Principle:

Answer 31

Capillary Electrophoresis

Question 32

STR Engraftment Analysis/Chimerism Sample requirement:

Answer 32

Buccal Swabs 2x 4mL EDTA

Question 33

For monitoring of transplant patients

Answer 33

STR Engraftment Analysis/Chimerism

Question 34

STR Engraftment Analysis/Chimerism Machine:

Answer 34

ABI 3500 Genetic Analyzer

Question 35

An infection disease caused by the SARS-CoV-2 virus

Answer 35

Coronavirus Disease (COVID-19)

Question 36

Coronavirus Disease (COVID-19) is formerly known to be

Answer 36

2019-nCoV

Question 36

Positive sense single-stranded RNA (+ssRNA) viruses with a crown-like appearance under an electron microscope due to the presence of spike glycoproteins on the envelope

Answer 36

Coronavirus Disease (COVID-19)

Question 37

Coronavirus Disease (COVID-19) Sample collection:

Answer 37

Nasopharyngeal ad Oropharyngeal swabs

Question 38

Coronavirus Disease (COVID-19) Machine:

Answer 38

Nextractor

Question 39

Automated system for rapid isolation of DNA and RNA form a variety of samples

Answer 39

Nextractor

Question 40

Was developed to amplify, isolate or identify RNA sequences

Answer 40

Reverse Transcription PCR (RT-PCR)

Question 41

The principle is to convert RNA into its complementary DNA sequence by reverse transcriptase, to synthesize a second strand with DNA polymerase, and finally to generate a ds cDNA molecule which can be amplified by PCR in the normal way

Answer 41

Reverse Transcription PCR (RT-PCR)

Question 42

Reverse Transcription PCR (RT-PCR) Machine:

Answer 42

Biorad Cfx 96 Real Time System

Question 43

Reverse Transcription PCR (RT-PCR) Reagent:

Answer 43

Sansure Biotech (Novel Coronavirus (2019-nCoV) Nucleic Acid Diagnostic Kit)