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Ch 1 Microscopes and Staining Flashcards

(11 cards)

1
Q

Hans Janssen and son Zacharias Janssen

A

invented first compound microscope in 1590

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2
Q

Galileo Galilei

A

developed occhiolino or compound microscope with a convex and a concave lens

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3
Q

Anton van Leewenhoek

A

-credited for bringing microscope to the attn. of biologist, even though lenseswere already being produced in the 1500s

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4
Q

What type of microscope generally used?

A
  • Compound, bright-field microscope

- Watson “Service” developed in early 1900s

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5
Q

phase contrast M

  • interference microscope
  • differential interference microscope
A

examine unstained cells and tissues still living

  • quantification of tiss mass
  • assessing surface properties of cells
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6
Q

Dark-field M

A
  • only light that’s been scattered or diffracted by structures in spec reaches objective
  • useful in examining autoradiographs
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7
Q

Flourescence

A
  • makes use of ability of certain molecules to fluoresce under UV light
  • various filters used
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8
Q

Confocal M

A

to visualize in very thin optical sections, like optical dissection

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9
Q

UV M

A
  • detecting nucleic acids and proteins

- uses quartz lens with UV light source

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10
Q

Polarizing M

A
  • highly ordered molecules or arrays of molecules can rotate angle of plane of polarized light, seeing birefringence (double refraction)
  • striated muscle, testicular interstitial cells
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11
Q

Electron M

  • TEM
  • SEM
A
  • like light M, but using e-
    • those e- passed, lighter than those absorbed or scattered.
    • freeze fracture

-e- beam doesn’t pass through specimen but is scanned across surface

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