Flashcards in Ch 2. Cells Involved in Immune Response Deck (73):
NKT cells have what type of TCR?
V alpha 24/J alpha 18, V beta 11
Tfh subtype are induced by what cytokine?
IL-21 is important for?
Tfh differentiation, production of antibody in germinal centers
CD marker for IL-2Ra
Transcription factors for Th1 differentiation?
T-bet, STAT 4
Transcription factors for Th2 differentiation?
GATA-3, STAT 6
Transcription factors for Th17 differentiation?
RORyT, STAT 3
Transcription factors for Tfh differentiation?
Bcl-6 (upregulates CXCR5, down regulates CCR7)
Transcription factors for Treg differentiation?
FoxP3, STAT 5
Th1 differentiation induced by?
Th2 differentiation induced by?
Th17 differentiation induced by?
Th9 differentiation induced by?
Treg differentiation induced by?
Th1 produce what major cytokine?
Th2 produce what major cytokine?
IL-4, -5, -13
Th17 produce what major cytokine?
Th9 produce what major cytokine?
Tfh produce what major cytokine?
Treg produce what major cytokine?
Difference between nTreg and iTreg
nTreg are thymus derived and express FoxP3, CD4, CD25. iTreg are peripheral derived, no FoxP3
cytokine for NK development
cytokine needed for proliferation of T cell progenitors
CD marker for common gamma chain
CCR 7 binds?
CCL 19, 21 (high conc in T cell parafollicular zone)
CXCR 5 binds?
CXCL 13 (high conc in B cell follicle)
Pro-B cell surface markers
CD 43, 19, 20
Pre-B cell surface markers
CD 43, 19, 20; pre-BCR (u heavy chain and surrogate light chain)
Immature naive B cell markers
CD 19, 20, IgM
Mature naive B Cell markers
CD 19, 20, IgM, IgD
double negative T cells in development express what markers?
CD3, preTCR (B-chain, pre-T alpha chain)
Double positive T cells express?
CD3, CD4, CD8, TCRab
Bim binds bax & bak and activates which caspase?
FasL (CD178) and Fas (CD95) binds and activates which caspase?
both caspase 8 and 9 converge on which caspase for apoptosis?
monocytes and macrophages express what CD marker?
CD14, a component of TLR4 and LPS receptor (note: CD33 on macrophages)
major cytokines secreted by macrophages
TNF-a, IL-1, IL-6, IL-12
mutation in XLP?
SH2D1A gene mutation (encodes SAP)
EBV infection in XLP can lead to?
marker for Langerhans cells
CD11c, Birbeck granules, CD1a and CD207
unlike other dendritic cells, pDCs express?
CD123 and NO CD11c!
FcER1 is expressed primarily on what cells?
mast cells and basophils
what is the function of PGE2
bronchodilation, suppress mast cells and eos, blocks 5-LO activation
function of LTC4, LTD4, LTE4
increase vasc permeability, bronchoconstriction, mucus secretion
location of MCt
respiratory, GI mucosa
location of MCtc
respiratory/GI submucosa, skin, eye, synovium, blood vessels
Which MC type has C5aR and responds to C5a, C3a, opiates and vancomycin and has a whorled appearance?
composition of high affinity IgE receptor on mast cells and basophils?
one alpha, one beta, two gamma chains
How is the high affinity IgE receptor different on dendritic cells, monocytes, and eosinophils?
no beta chain (one alpha, two gamma chains)
CD marker for low affinity IgE receptor?
which allergen cleaves CD23 (low affinity IgE receptor?)
Der p 1
What percent reduction is needed in specific IgE for clinical efficacy of Xolair?
What autoAb can be found in CIU?
IgG against alpha chain of high affinity receptor
What chain of the high affinity IgE receptor binds IgE?
Which cytokine is important for basophil expansion/differentiation
IL-3 (binds CD123 the IL-3 receptor)
eosinophils migrate to tissue in response to?
RANTES (CCL5), eotaxin 1, -2 (CCL11, CCL24)
Eosinophil levels are suppressed by?
primary cause of eosinophilia in U.S.? worldwide?
mediators that attract neutrophils to tissue?
IL-8 (CXCL8), LTB4
antibodies to what antigen in HIT?
PF4 (platelet antigen)
antibodies to what antigen in ITP?
antibodies to what antigen in TTP?
eczema, thrombocytopenia, infections
target antigen and immunoflourescense for pemphigus vulgaris
desmoglein 3, intercellular IgG,IgM
target antigen and immunoflourescense for bullous pemphigoid
BP230 (BPAg1), BP180 (BPAg2), linear IgG at BMZ
target antigen for dermatitis herpetiformis
transglutaminase, granular IgA in dermis
target antigen for linear IgA dermatosis
BP180 (BPAg2), linear IgA at BMZ
what mediators released from epithelial cells are important in Th2 polarization?
E-selectin and P-selection on endothelium bind to?
Sialyl-Lewis X on leukocytes
L-selectin on leukocytes bind to?
Sialyl-Lewis X on GlyCAM-1/MadCAM-1 on HEV
ICAM on endothelium bind to?
LFA-1 (CD11aCD18 integrin) on leukocytes
VCAM on endothelium bind to?