Ch 2.3 Dna Replication Flashcards
1
Q
Why is replication semi conservative?
A
Each daughter has one new strand one parent strand
2
Q
What is DNA polymerase?
5
A
- large enzyme complex
- uses template strand to add complementary sequence on new strand
- reads dna 3-5
- makes new strand 5-3
- Needs OH from primer to start
3
Q
Circular dna replication?
A
Starts at origin I’d replication (oriC) till both sides meet
4
Q
Liner dna replication
A
Multiple origins of replication (OriR)
5
Q
How is the dna is separated for replication?
A
- unwinds a bit at a time
- Helicase: unwinds
- topoisomerase: cuts to release stress
6
Q
How primers are made for replication
A
-Uses RNA primase ( spect ion rna pol)
7
Q
Leading vs lagging strand
A
Leading: continus
Lagging: fragments
8
Q
Lagging strand
A
- new rna primer
- Okazaki fragments
- primers are replaced by DNA polymerase 1
- connected with DNA ligase
9
Q
What does replication look like?
A
The lagging strand loops around so replication can continue in the same direction
