CH 3Lab Nucleic Acids and The Encoding of Biological Info Flashcards Preview

Final Exam > CH 3Lab Nucleic Acids and The Encoding of Biological Info > Flashcards

Flashcards in CH 3Lab Nucleic Acids and The Encoding of Biological Info Deck (18):
1

What is the main enzyme for making a polymer of RNA?

RNA polymerase

2

How does the enzyme above recognize where the information for a gene is?

The promoter region known as the TATA box

3

Where does transcription and translation occur in a eukaryotic cell?

Nucleus and the cytoplasm respectively

4

Where does transcription and translation occur in a prokaryotic cell?

Both occur in the cytoplasm

5

Where does transcription end?

It ends at the 3' TTATTT 5' sequence

6

What is transcription and why is it done?

Process of copying DNA into RNA Used to blueprint for protein synthesis

7

What are the 4 molecular factors required for transcription?

RNA polymeraseActivator proteinsEnhancerMediator complex

8

What are the three phases of transcription?

InitiationElongation Termination

9

What are introns?

Non coding regions. Removed during transcription

10

What are exons?

Coding regions. Kept during transcription

11

Where does translation occur in a eukaryotic cell?

In the cytoplasm

12

What are the two roles of tRNA ^MET in the initiation process?

Finds the start siteInitiates brining in other amino acids

13

What is the role of the 5' cap.

Scans for UAG

14

What is happening at the A, P and E sites respectively?

At the A site tRNA moves in and attaches to codonAt the P site transfer of amino acids to form an amino acid chainAt the E site the tRNA exits the site

15

How does translation end?

When it reaches the stop codon UAG

16

What are the molecular factors required for translation?

mRNA, release factors, aminoacyl tRNA synthetase, tRNA and ribosomes

17

Where does mRNA translation begin?

At the 5' cap

18

What molecule is the source of information for transcription?

DNA