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chap 11 Flashcards

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1
Q

are the growth of microorganism in a culture medium

A

culture

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2
Q

culture medium is composed of mixture of nutrients such as

A

carbon, nitrogen, sulfur, phosphorus, hydrogen, oxygen, and buffer

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3
Q

patterns of microbial growth

A

liquid, semi-solid, solid medium

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4
Q

these inhibitory agents are added into medium to facilitate the isolation of desired bacteria while suppressing other microorganism presents in the sample

A

dyes, salt, and antimicrobials

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5
Q

if several plates will be inoculated, media should be arranged from__ to __

A

most enriched to selective

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6
Q

isolation/identification of microorganism in the areas of industrial and food microbiology requires

A

specialized culture media

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6
Q

types of culture

A

pure
mixed
stock culture

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7
Q

contains a single species

A

pure culture

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8
Q

more than one species

A

mixed culture

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9
Q

composed of several species contained in a separate culture medium

A

stock culture

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10
Q

should be cultivated in a large volume of broth and divided small vials to lengthens the shelf life of culture for a year

A

stock culture

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11
Q

used for academic and industrial purposes

A

stock culture

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12
Q

classification of culture media
according to consistency

A

liquid medium
semi-solid medium
solid medium

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13
Q

does not contain any amount of agar

A

liquid medium

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14
Q

liquid medium allow the growth of

A

aerobes, anaerobes, and facultative anaerobes

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15
Q

ex; of liquid medium

A

brain heart infusion
trypticase soy broth
thioglycollate

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16
Q

semi solid medium contain ___ agar

A

0.5 % to 1% agar

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17
Q

used to observed bacterial motility and detect indole and sulfide production

A

semi solid medium

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18
Q

ex semi solid

A

sulfide indole motility medium

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19
Q

solid medium contains __ agar

A

2% to 3% agar

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20
Q

ex. of solid medium

A

triple sugar iron agar
macconkey agar
blood agar plate
chocolate agar plate

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21
Q

a sulfated polymer

A

agar

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22
Q

agar is made up of

A

d-galactose
3,6-anhydro-L-galactose
D glucuronic acid

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23
Q

agar is usually derived from

A

red algae

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24
cooling temp of culture medium to petrinplates is
55-60 degree celsius
25
melting temp of agar __
80-90 degree celsius
26
agar solidify at
40-50 degree celsius
27
molten agar in amount of ___ should be transferred to __
20-25 mL STERILEPLATES
28
according to composition
synthetic or defined medium non synthetic or complex medium tissue culture medium
29
medium which all components are known
synthetic or defined medium
30
designed for research purposes either liquid or solid medium
synthetic or defined medium
31
preferred for the isolation of cyanobacteria and chemoorganotrops
synthetic or defined medium
32
synthetic or defined medium is preferred for isolation of
cyanobacteria and chemoorganotrophs
33
synthetic or defined medium ex
BG-11 medium
34
medium which some of substance are unknown
non synthetic or complex medium (peptone, yeast extract, and meat)
35
useful for isolation of medically significant bacteria
non synthetic or complex medium
36
non synthetic or complex medium ex
nutrient broth medium trypticase soy broth macconkey agar
37
utilized for obligate intracellular bacteria
tissue culture medium (rickettsia and chlamydia)
38
ex tissue culture medium all of these ex are used for isolation of
heLa 229 cells- human cervical tissue cells mcCoy cells and w 138 cells - fibroblast chlamydia
39
utilized for propagation of rickettsia
embryonated eggs
40
plated media are distributed into __
dish or plate
41
tube media prepared either
liquid, slant, buut, and slant or butt
42
according to dispensing what are the examples
triple sugar iron agar sulfide indole motility medium simmon's citrate agar lysine iron agar
43
routine in laboratory without additional supplements
supportive media/simple media
44
media that support most non fastidious bacteria
supportive media/simple media
45
supportive media/simple media is usually composed of
meat and soybean extracts
46
ex supportive media/simple media
nutrient agar trypticase soy broth nutrient broth
47
propagate certain group of bacteria from mixture of organism
enrichment mediacon
48
contain specific nutrients and without additional supplements
enrichment media
49
these media are incubated for certain periods then subculture to isolate desired organism
enrichment media
50
used as supplement to agar plate to detect aerobes, anaerobes, microaerophiles
enrichment media
51
enrichment media ex.
gram negative broth alkaline peptone water
52
alkaline peptone water adjusted to ph__ which is best for ___ before inoculation into __
8.4 vibrio spp. thiosulfate citrate bile-salt-sucrose agar
53
selenite F is for isolation of __
salmonella
54
__ is for isolation of salmonella
selenite f
55
general support enrichment medium that promotes growth of almost all non-fastidious bacteria
thioglycollate
56
enrichment broth for salmonella and proteus
tetrathionate
57
tetrathionate is for isolation of
salmonella and proteus
58
it is both enrichment and selective medium that contains sodium citrate and sodium deoxycholate
gram negative broth w/c inhibit gram positive bacteria
59
enrichment for group B streptococci
lim broth
60
lim broth is for isolation of
group B streptococci
61
media with additional supplements such as blood, vit, yeast extract for growth of fastidious organism and ex.
enriched media and non selective media BAP (BLOOD AGAR PLATES) AND CAP (COLUMBIA BLOOD AGAR
62
solid type of media
enriched media and non selective media
63
utilized to differentiate hemolytic patterns of bacteria
BAP (BLOOD AGAR PLATES)
64
used for recovery of haemophilus spp.
columbia blood agar
65
promote isolation of fastidious organism
blood agar plates columbia blood agar
66
this media allows visualization of metabolic differences between groups of media
differential media
67
ph indicator of macconkey agar
neutral red
67
__ separates lactose fermenters ___ from non lactose fermenters ___
macconkey agar pink colonies colorless colonies
68
___ Characterized bacteria and recommended for urine culture
cystine lactose electrolyte deficient agar yellow colonies
69
media incorporated with antibiotics, dyes/chemicals to inhibit growth of other organisms while promoting growth of desired bacteria ex
selective media MAC
70
to inhibit gram positive bacteria
crystal/ gentian violet,basic/carbon fuchsin and bile salts
71
to inhibit gram negative bacteria
potassium tellurite and sodium azide
72
to inhibit swarming of swarming bacteria
alcohol and chloral hydrate
73
used to isolate bacteria with specific growth requirement ex.
special media thiosulfate citrate bile salt sucrose agar lowenstein jensen medium
74
composed of whole eggs and malachite green
lowenstein jensen medium
75
supports growth through inspissation and not autoclaving
lowenstein jensen medium
76
selective for sterilization through boiling and not autoclaving
thiosulfate citrate bile salt sucrose agar for vibrio spp.
77

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