chap 14

Question 1

wet mount and hanging drop preparation

Answer 1

motility test

Question 2

stainings are

Answer 2

gram staining- acid fast staining- structural staining

Question 3

comsist of plastic strips and microtubes that contain dehydrated biochemical substrates

Answer 3

analytical profile index

Question 4

commony used for identification of gram negative enteric bacteria

Answer 4

analytical profile index

Question 5

analytical profile index is incubated for

Answer 5

18-24 hours at 35 degree celsius

Question 6

in this method known pattern of microbial growth are compared with test organisim using computer software

Answer 6

automated method for identification of bacteria

Question 7

pure bacterial isolate during incubation at

Answer 7

35 DC for 16-24 hours

Question 8

producing acid or alkaline end products as early as

Answer 8

2 hours

Question 9

a basic detection of antibody response from antigenic simulatuon

Answer 9

serological test

Question 10

one diagnosic test for organism that are difficult to culture - atypical bacteria

Answer 10

serological test

Question 11

performed by adding antibodies to bacter suspension thay will bind to surface of organism

Answer 11

bacterial agglutination

Question 12

used to identify bacteria that are difficult to cultivate in culture medium

give ex.
positive results

Answer 12

bacterial agglutination
widal test
visible clumps/agglutination

Question 13

uses artificial carriers such as katex particles or treated RBC or biological carrier like bacterial cells as reagent which can adsorb test antigen and react with specific antibody

Answer 13

particle agglutinations

Question 14

in this test, antigen in patient specimens bind to antibodies that present in surface of latex beads

Answer 14

latex agglutination

Question 15

uses antubody bound to particle to increase visibility or agglutination reactions btween antigen and antibody

Answer 15

coagglutination

Question 16

composed of two parts that test systems include antigen causes the diseases and indicator which consist sheeps red blood cells, complement fixing antibody

Answer 16

complement fixation

Question 17

uses soluble antigen that reacting with antibodies in serum sample

Answer 17

flocculation tests

Question 18

sensitive and specific method for detection of antibodies against certain pathogens

Answer 18

ELISA enzyme linked immunosorbent ASSAY

Question 19

commonly used for rapid identification of bacterial and viral antigens

Answer 19

immunofluorescent assay

Question 20

utilizef fluorescent dye and specific kabeled antibodies

Answer 20

direct FAT

Question 21

two step or sndwich tech thaht is mor sensitive than other test of FA

Answer 21

Indirect FAT

Question 22

based on electrophoretic separation of bacterial protein in supporting media

Answer 22

western blot

Question 23

strain typing tech that useful addition to epidemiologic research

Answer 23

pulsed field gel electrophoresis

Question 24

virus that attack’s bacteria

Answer 24

bacteriophage

Question 25

this method is based on specificity of phage surface receptors for cell

Answer 25

bacteriophage typing

Question 26

currently considered most important method for microbial identification

Answer 26

molecular diagnosis

Question 27

most commonly used amplification tech in molecular diagnosis

Answer 27

polymerase chain reaction

Question 28

a dye that binds to double stranded DNA but not tonsingle stranded

Answer 28

SYBR green

Question 29

determine the order kf nucleotides in DNA fragments

Answer 29

DNA sequencing

Question 30

molecular approach that does not necessiate nuclear acid amplification has h specificity and h sensitivity

Answer 30

fluorescence in situ hybridization

Question 31

refers to grouping of DNA at micro or nano level

Answer 31

microarrays and nanoarrays

Question 32

detect gene expression of target microorganisms, identifies bacte, new gene and mutation

Answer 32

microarrays and nanoarrays

Question 33

It is the study ofmicrobialgenes collectedfrom the environment.

Answer 33

metagenomics

Question 34

is useful in the study of the indigenous flora in the human body and gut

Answer 34

metagenomics

Question 35

Itis the study of proteins (proteome) on cellular level.

Answer 35

proteomics

Question 36

In clinical microbiology, it identifies protein expression i n biosamples suspected with pathogens. It also determines the effectiveness of drug therapy across diseases.

Answer 36

proteomics