Chap 8: Analytical Methods and Validity test Flashcards
(13 cards)
Techniques use for Screening
- Immunoassay
- Chromatography
It is based on antigen-antibody reactions
Immunoassay
Example of Immunoassay
- EIA
- EMIT
-Fluorescence polarization - Radioimmunoassay (RIA)
Separation of a mixture is the main outcome of this method
Chromatography
Example of Chromatography
- TLC
- GC
- HPLC
Initial validity test
- Physical appearance
- Urine SG, pH and nitrites
- Urine Creatinine
- Presence of oxidizing adulterant
The specimen contains exogenous substance at a concentration greater than what is considered a normal physiological concentration
Adulterated
Adulterated spx is confirmed when the nitrite is
> /= to 500 ug/L
Adulterated spx is confirmed when the pH is
< 3 or = to 11
Substituted urine has a creatinine concentration of ___
< 442.0 umol/L
Substituted urine has a SG of ___
< 1.002 or >/= 1.020
Diluted urin is confirmed when the creatinine concentration is ___
< 1768.0 umol/L
Diluted urin is confirmed when the SG is ___
<1.003