CHAPTER 1 Flashcards

Liquid handling tool selection (52 cards)

1
Q

Meant for general use with aqueous solutions

A

Air displacement pipettes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Two types of pipettes

A

Air displacement pipettes
Positive displacement pipettes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Used for highly viscous and volatile
liquids

A

Positive displacement pipettes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Highly accurate for standard pipetting applications

A

Air displacement pipetting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

May affect the performance of air displacement pipettes

A

Temperature
Atmospheric pressure
Specific gravity
Viscosity of the solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

This is based on direct contact of the piston with the liquid

A

Positive displacement pipetting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

In air displacement pipettes the tips contain both the cylinder/capillary and the piston (T/F)

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

In positive displacement pipettes, a certain volume of air remains between the piston and the liquid. (T/F)

A

F

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

In positive displacement pipetting, the piston is in direct contact with the liquid. (T/F)

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

The type of experiment you are performing and the physical properties of the liquid will determine the correct pipette tip to use. (T/F)

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

A multi-purpose tip for many laboratory applications with a variety of performance requirements that range from high accuracy to reagent dispensing with greater tolerance

A

Standard Tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

This is available for applications demanding the highest level of purity

A

Sterile standard tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Beneficial when the assay is sensitive to cross-contamination, or if the sample can contaminate the lower part of the pipette

A

Filter tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

This prevents liquid from accidentally splashing the inside of the pipette, and reduces aerosols from penetrating the pipette tip cone during pipetting

A

Filter tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

This is recommended for low volume applications in genetic studies, forensics, PCR, and radioisotope sampling

A

Filter tip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Filter tips are recommended for low volume applications in …

A

Genetic studies
Forensics
PCR
Radioisotope sampling

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Filter tips are available with either … – both of which are designed to prevent cross-contamination

A

Self-sealing barrier
Standard filter tips

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Filter tips are available with either self-sealing barrier or standard filter tips – both of which are designed to

A

Prevent cross-contamination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

This allow you to access the bottom of test tubes, reagent bottles, flasks, and other vessels without touching the shaft of the pipette against the side of the tube.

A

Extended length tips

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

This adds a layer of security to protect samples, and virtually eliminates the chance of carryover contamination.

A

Extended length tips

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

The longer tip length allows you to reach the bottom of long or narrow vessels that standard tips cannot reach (T/F)

22
Q

The shorter tip length allows you to reach the bottom of long or narrow vessels that standard tips cannot reach (T/F)

23
Q

This is designed for unique pipetting applications to save time, reduce contamination, and increase accuracy, precision, and productivity

A

Specialty tips

24
Q

Specialty tips are designed for unique pipetting applications to

A

Save time
Reduce contamination
Increase accuracy, precision, and productivity

25
Utilizing polymer technology makes the inner surface of the pipette tip more hydrophobic, resulting in a significant reduction in sample loss due to adhesion.
Low retention pipette tips
26
Benefits include improved sample delivery and conservation of expensive reagents
Low retention pipette tips
27
Benefits of low retention pipette tips
Improved sample delivery Conservation of expensive reagents
28
This tip feature a distal end orifice that is nearly 70 percent larger than that of a standard pipette tip
Wide orifice tip
29
This provide the added flexibility required for handling difficult-to-pipette samples
Wide orifice tip
30
This is designed for researchers working with macromolecules like genomic DNA and are especially critical for transferring fragile cellular samples such as macrophages, hybridomas, and hepatocytes, as well as other viscous materials
Wide orifice tip
31
Tips
Standard tips Filter tips Extended length pipete tips Long retention pipette tips Specialty tips Wide orifice tips Gel Loading Tips Solvent Safe Carbon Filtered Tips Individually Wrapped Tips
32
Tips used for agarose gels
Round gel loading tips
33
Tips used for your polyacrylamide gels
Specialized Ultra Round and Ultra Flat gel tips
34
Best solution for handling the pipetting rigors of Combinatorial Chemistry.
Solvent Safe Carbon Filtered Tips
35
These specialized tips keep strong acids, bases, and aggressive organic solvents from causing pipette failures and critical inaccuracies.
Solvent Safe Carbon Filtered Tips
36
This is for extremely sensitive applications
Individually Wrapped Tips
37
This is deal for extremely sensitive applications requiring strict aseptic conditions
Individually Wrapped Tips
38
Due to their versatility and wide volume range these are the most used laboratory instruments
Variable volume air displacement single-channel pipettes
39
Most commonly used in microplate applications, such as ELISA, PCR, or cell culture.
Multichannel pipettes
40
Offer instant usability for small scale multichannel work.
Manual multichannel pipettes
41
These are available as 8- or 12-channel versions to work with 96-well microplates, and as a 16-channel version to work with 384-well microplates.
Multichannel pipettes
42
These are versatile laboratory workhorses that can be programmed to perform most laboratory tasks.
Electronic pipettes
43
The most commonly used function of electronic pipettes is the ..
Aliquoting of a reagent into multiple doses - MULTIDISPENSING
44
By using a multichannel electronic pipette and repeat dispensing modes in microplate filling, the time needed for filling the plate can be reduced from several minutes to less than a minute. (T/F)
T
45
Electronic pipettes that offer adjustable tip spacing increase efficiency in samples transfers to move several samples at once between different labware formats. (T/F)
T
46
These are used in cell and tissue culture applications, and in general laboratory liquid dosage when more than 1 ml volumes are pipetted.
Serological pipets
47
These are made of glass or polystyrene. Plastic, disposable pipets are useful in applications where sterility is a requirement.
Serological pipets
48
Help to aspirate and dispense liquids accurately and with precision.
Serological Pipet Fillers
49
In serologic pipets, the speed of both aspiration and dispensing can be adjusted separately to work with a variety of liquids. (T/F)
T
50
This is ideal for simple repetitive tasks with a dispensing range up to 5 ml.
Manual Stepper pipettes
51
This is a reliable and easy tool for dispensing reagents directly from the reagent bottle.
Bulk reagent dispenser
52
This offers speed and accuracy with no extra work steps in everyday liquid dispensing.
Bulk reagent dispenser