Specimen Storage Requirements for Nucleic Acid Extraction

Question 1

DNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP?:24 hours

Answer 1

22°–25°C

Question 2

DNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP?:72 hours *

Answer 2

2°–8°C

Question 3

DNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP?: At least 1 year *

Answer 3

–20°C

Question 4

DNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP?:More than 1 year *

Answer 4

–70°C

Question 5

DNA

Whole blood, buffy coat,
bone marrow, fluids
22°–25°C : TIME?

Answer 5

24 hours

Question 6

DNA

Whole blood, buffy coat,
bone marrow, fluids
2°–8°C : TIME?

Answer 6

72 hours *

Question 7

DNA

Whole blood, buffy coat,
bone marrow, fluids
–20°C : TIME?

Answer 7

At least 1 year *

Question 8

DNA

Whole blood, buffy coat,
bone marrow, fluids
–70°C : TIME?

Answer 8

More than 1 year *

Question 9

RNA

Whole blood, buffy coat,
bone marrow, fluids
22°–25°C

Answer 9

Not recommended

Question 10

RNA

Whole blood, buffy coat,
bone marrow, fluids
2°–8°C: TIME

Answer 10

2–4 hours *

Question 11

RNA

Whole blood, buffy coat,
bone marrow, fluids
–20°C: TIME

Answer 11

2–4 weeks *

Question 12

RNA

Whole blood, buffy coat,
bone marrow, fluids
–70°C : TIME

Answer 12

More than 1 year *

Question 13

RNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP ; 2–4 hours *

Answer 13

2°–8°C

Question 14

RNA

Whole blood, buffy coat,
bone marrow, fluids
TEMP ; –20°C

Answer 14

2–4 weeks *

Question 15

RNA

Whole blood, buffy coat,
bone marrow, fluids
TIME ; –70°C

Answer 15

More than 1 year *

Question 16

RNA

Fluids collected in specialty
22°–25°C ; TIME

Answer 16

5 days

Question 17

RNA

Fluids collected in specialty
TEMP ; 5 days

Answer 17

22°–25°C

Question 18

RNA

Fluids collected in specialty
TEMP ;TIME

Answer 18

22°–25°C : 5 days

Question 19

RNA

RNA protection tubes
2°–8°C ; TIME

Answer 19

7 days

Question 20

RNA

RNA protection tubes
–20°C ; TIME

Answer 20

2–4 weeks

Question 21

RNA

RNA protection tubes
–70°C ; TIME

Answer 21

At least 7 months

Question 22

RNA

RNA protection tubes
TEMP ; 7 days

Answer 22

2°–8°C

Question 23

RNA

RNA protection tubes
TEMP ; 2–4 weeks

Answer 23

–20°C

Question 24

RNA

RNA protection tubes
TEMP ; At least 7 months

Answer 24

–70°C

Question 25

RNA Tissue 22°–25°C

Answer 25

Not recommended

Question 26

RNA Tissue 2°–8°C

Answer 26

Not recommended

Question 27

RNA Tissue –20°C

Answer 27

Not recommended

Question 28

RNA Tissue –70°C ; TIME

Answer 28

At least 2 years

Question 29

RNA Tissue –140°C (nitrogen vapor) ; TIME

Answer 29

At least 2 years

Question 30

RNA Tissue TEMP ; At least 2 years

Answer 30

–70°C

Question 31

RNA Tissue TEMP ; At least 2 years

Answer 31

–140°C (nitrogen vapor)

Question 32

RNA Cell lysates in GITC TEMP ; 1–2 weeks

Answer 32

22°–25°C

Question 33

RNA Cell lysates in GITC TEMP ; TIME

Answer 33

22°–25°C ; 1–2 weeks

Question 34

RNA Cell lysates in GITC 22°–25°C ; TIME

Answer 34

1–2 weeks

Question 35

RNA Cell lysates in RNA storage solution (Ambion) TEMP ; 1 week

Answer 35

22°–25°C

Question 36

RNA Cell lysates in RNA storage solution (Ambion) 22°–25°C ; TIME

Answer 36

1 week

Question 37

RNA Cell lysates in RNA storage solution (Ambion) TEMP ; 1 month

Answer 37

2°–8°C

Question 38

RNA Cell lysates in RNA storage solution (Ambion) TEMP ; More than 1 year

Answer 38

–20°C

Question 39

RNA Cell lysates in RNA storage solution (Ambion) 2°–8°C ; TIME

Answer 39

1 month

Question 40

RNA Cell lysates in RNA storage solution (Ambion) –20°C ; TIME

Answer 40

More than 1 year

Question 41

RNA Microorganisms in culture 22°–25°C ; TIME

Answer 41

24 hours

Question 42

RNA Microorganisms in culture 2°–8°C ; TIME

Answer 42

72 hours

Question 43

RNA Microorganisms in culture –20°C ; TIME

Answer 43

2–4 weeks

Question 44

RNA Microorganisms in culture –70°C ; TIME

Answer 44

More than 1 year

Question 45

RNA Microorganisms in culture TEMP ; 24 hours

Answer 45

22°–25°C

Question 46

RNA Microorganisms in culture TEMP ; 72 hours

Answer 46

2°–8°C

Question 47

RNA Microorganisms in culture TEMP ; 2–4 weeks

Answer 47

–20°C

Question 48

RNA Microorganisms in culture TEMP ; More than 1 year

Answer 48

–70°C