Chapter 10 Blood Collection Procedures

Question 1

Plasma

Answer 1

Liquid portion of the UNCLOTTED blood and still contains clotting factors

Question 2

Serum

Answer 2

liquid portion of blood that has been allowed to clot (coagulate). Clotting factors are no longer present as they have been used to clot the blood.

Question 3

Tubes with anticoagulants

Answer 3

Light blue
Green (light or dark)
Pink
Lavender
Royal blue with a lavender stripe
Gray

Question 4

What will tubes with anticoagulants produce?

Answer 4

Plasma when separated in a centrifuge

Question 5

Tubes without anticoagulants List (4)

Answer 5

Clear-used as a discard tube
Red-glass
Tiger Top/Gold Top/ Marble Top/ SST
Royal blue with red stripe

Question 6

What will tubes without anticoagulants produce?

Answer 6

Serum when separated in a centrifuge

Question 7

What is the order of draw

Answer 7

1. Blood Culture Bottles (Broth) BCX2
2. Clear or Red (glass)/Light blue top (Sodium Citrate) 100% full
3. Royal Blue with Red Stripe (none)
4. Red top (Clot Activator if plastic)
5. Gold, Tiger, Marble, SST (Silica/Gel)
6. Light green top (Lithium Heparin/Gel) (PST)
7. Dark Green Top (Sodium Heparin) *put on ice
8. Pink Top (Potassium EDTA) 2/3 full
9. Lavender top (EDTA)
10. Royal Blue w/Lavender Stripe (EDTA)
11. Gray Top (Sodium Fluoride / Potassium Oxalate)

Dark Blue (As called for in draw order)

Question 8

1. Blood Cultures

Answer 8

x2
Additive-Nutrient Broth
Lab responsible for specimen testing: Microbiology

Each set consists of two bottles
Aerobic (w/oxygen)
Anaerobic (w/out oxygen)

Question 9

2. Clear Top Tube or Red top tube

Answer 9

Discard to avoid Thrombin and other cells

Question 10

2. Light Blue Top Tube

Answer 10

Additive: Sodium Citrate
Yields: Plasma
Lab responsible for specimen testing: Coagulation or Hematology

9 parts blood to 1 part additive (filled completely)
3-4 times inverted

Question 11

Common Tests for Light Blue

Answer 11

Prothrombin Time (PT)-
Partial Thromboplastin Time (PTT)-
Activated Partial Thromboplastin Time (aPTT)

D-Dimer

Question 12

4. Red Top Tube Clot

Answer 12

Additive: Glass-none
Plastic-clot activator (S)ETOH
Yields: Serum

Lab responsible for specimen testing:Chemistry

Question 13

Common Tests for Red Top Tube

Answer 13

“Plan vacuum tube” no addditve or anticoagulant. In this tube, blood will clot naturally within 30-60 minutes

serum hCG
serum ETOH

Question 14

5. Tiger/Marble/Red-Gray Speckled, Gold or SST Top Tube additives

Answer 14

Additive: Silica (clot activator) & Thixotropic Gel (serum separator)
Yields: Serum

Lab responsible for specimen testing: Chemistry

Question 15

Common Tests for SST Tube

Answer 15

Basic Metabolic Panel (BMP)
Complete/Comprehensive Metabolic Panel/Profile (CMP)
Serum hCG
Lipids/Cholesterol
Hepatic Panel
Progesterone

Question 16

6. Light (Lithium Heparin) Green Top Tube (PST)
   Additives

Answer 16

Additive: Lithium Heparin (an anticoagulant), and Thixotropic Gel (a plasma separator)
Yields: Plasma

Lab responsible for specimen testing: Chemistry

Question 17

Common Tests for Light PST (LITHIUM HEPARIN & THIXOTROPIC) green top tube

Answer 17

STAT Electrolytes “LYTES”-

(P) hCG

(P) hCG qualitative-

(P) hCG quantitative

Question 18

7. Dark Green Top Tube (Sodium Heparin)

Answer 18

Additive: Sodium Heparin an anticoagulant
Yields: Plasma

Lab responsible for specimen testing: Chemistry

Question 19

Common tests for Dark Green Top Tube

Answer 19

Ammonia

**Must be placed on ice

Question 20

8. Pink Top Tube (P) in (K2)
   Additives

Answer 20

Additive: Potassium EDTA
Yields: Plasma

Lab responsible for specimen testing: Blood Bank

2/3 full or more

Question 21

Common Tests for Pink Top Tube

Answer 21

Type and Screen Ratio (TSR)

Cross Match

Question 22

9. Lavender or Light/Dark Purple Top Tubes
   Additives

Answer 22

Additive: Ethylendiaminetetraacetic Acid (EDTA)
Yields: Plasma

Lab responsible for specimen testing: Hematology

Question 23

Common tests for Lavender or Light/Dark Purple Top Tubes (7)

Answer 23

Erythrocyte Sedimentation Rate (ESR)
Sickle Cell
Complete Blood Count (CBC) **never centrifuged
Hematocrit
Hemoglobin
Platelets
Hemoglobin A1C (HgA1C)- 3 month span for diabetes

Question 24

11. Gray Top Tube
    Additives

Answer 24

Additives: Sodium Fluoride an antiglycolytic agent (glucose preservative) and Potassium Oxalate an anticoagulant
Yields: Plasma

Lab responsible for specimen testing: Chemistry

Question 25

Common Tests for Gray Top Tube (3)

Answer 25

Oral Glucose Tolerance Test (OGTT) Lactic Acid Should be drawn without stasis (no tourniquet applied) **sample must be placed on ice Alcohol (P) ETOH- do NOT clean the site with alcohol

Question 26

Sodium Fluoride

Answer 26

Preservative that prevents the breakdown of sugar. It will preserve the integrity of glucose in the sample for 3 days

Question 27

If glucose test requires serum which tube top color should be used instead of Gray?

Answer 27

SST

Question 28

Potassium Oxalate

Answer 28

Is an anticoagulant that prevents clotting by binding to calcium in the sample.

Question 29

**Royal Blue Top Tube Both additives

Answer 29

Additive: Red Stripe-None Blue/Lavender Stripe-EDTA Yields: Red Stripe-Serum Blue/Lavender Stripe-Plasma Lab responsible for specimen testing: Toxicology

Question 30

**common Test for Royal Blue Top Tube

Answer 30

Lead (Pb) Poisoning Heavy Metal Toxicology

Question 31

3. Royal Blue Top Tube with Red Stripe No additive tests

Answer 31

Lead (Pb) Poisoning Heavy Metal Toxicology

Question 32

10. Royal Blue Top Tube with Blue/Lavender Stripe EDTA Tests

Answer 32

Lead (Pb) Poisoning Heavy Metal Toxicology

Question 33

What does the Royal Blue Top Tube with Red Stripe yield?

Answer 33

Serum

Question 34

What does Royal Blue Top Tube with Blue/Lavender stripe yield

Answer 34

Plasma

Question 35

TJC The Joint Commission

Answer 35

Solely look out for patients Establish standards and practices of ops in all healthcare settings Improve healthcare

Question 36

CLSI Clinical & Laboratory Standards Institute

Answer 36

Develops standards Sets standards globally nfp Phlebotomy programs approval, cert exam, questions and standard of care based on CLSI standards