Chapter 11 Flashcards

1
Q

What are the three methods to destroy or reduce undesirable microbes?

A

Physical, Chemical, and Mechanical

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1
Q

What are the highest resistance of microbes?

A

Prions, bacterial endospores

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2
Q

True or False?
Moderate resistance of microbes consist of Pseudomonas sp., Mycobacterium tuberculosis, Staphylococcus aureus, protozoan cysts.

A

True

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3
Q

Fill in the blank:
LEAST RESISTANCE
1) most bacterial ______ cells
2) _____ spores and ______, yeasts
3) enveloped __________
4) protozoan _________

A

1) Vegetative
2) fungal and hyphae
3) viruses
4) trophozoites

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4
Q

Define Sterilization

A

removal or destruction of all viable microbes

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5
Q

Define Germicide:

A

also called microbicide; any chemical agent that kills pathogenic microorganism, used on inanimate materials or on living tissue, but cannot kill resistant microbial cells

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6
Q

What term means “destruction of vegetative pathogens on inanimate objects”

A

Disinfection

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7
Q

What term means “chemicals applied to body surfaces to destroy or inhibit vegetative pathogens”

A

Antisepsis

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8
Q

Define Sanitization:

A

cleansing technique that removes microorganism and debris from inanimate surfaces

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9
Q

what does suffix “cide” mean

A

kill the bacteria

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10
Q

what suffix means to inhibit bacteria’s ability to produce

A

static

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10
Q

What is microbial death?

A

permanent loss of reproductive capability, even under optimum growth conditions

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11
Q

what are the 4 modes of action of antimicrobial agents

A

Cell wall, Cell membrane, Nucleic acid synthesis, Alter protein function

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12
Q

Fill in the blank:

Factors that govern the effectiveness of a particular agent
1) _______ time
2) Concentration the _____
3) _______ of organisms
4) __ of the environment
5) Mode of ______
6) Present of ________ or _______ substances

A

1) Exposure
2) agent
3) Number
4) PH
5) action
6) solvents or interfering

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13
Q

What are the 5 methods of Physical Agents

A

1) Heat - moist & dry
2) cold temperatures
3) Desiccation
4) Radiation
5) Filtration

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14
Q

What does Moist heat consist of?

A

Autoclaving, pasteurization, and boiling water

15
Q

Is pasteurization a method of sterilization?

A

No, it doesn’t sterilize it kills non-spore forming pathogens and lowers overall microbe count

16
Q

What’s the difference of between autoclaving and boiling water?

A

Autoclaving sterilizes and Boiling water disinfects

17
Q

What’s the difference between Dry and Moist heat

A

Dry heat uses higher temperatures

18
Q

What are 2 examples of Dry heat

A

Dry Oven - used for glassware, metals, powder, oils

Incineration - Bunsen burner for needles/loops

19
Q

What does refrigeration do?

A

slows the growth of microbes

20
Q

What is Desiccation?

A

removal of water from cells, lead to metabolic inhibition.
-used for foods: raisins, jerky
-Lyophilization: freeze drying/ “snap frozen”

21
Q

How does radiation work?

A

occurs as waves or particles bombard molecules in cell. damages DNA=mutations

22
Q

True or False
Ionizing radiation has a deep penetrating power and can penetrate paper & plastic. Can penetrate cells and breaks DNA. Also consists of Gamma rays (xrays)

A

True

23
Q

What kind of radiation is used to cut down microbial loads in medical equipment, loops, and gloves.

A

Ionizing radiation

23
Q

Define cold sterilization

A

sterilize materials that are sensitive to heat or chemicals

24
Q

Fill in the Blank

Nonionizing Radiation
1) ____ penetrating power
2) must be _____ exposed
3) used to _____ & _____
4) _________ rays
5) UV light creates ______ ______, which interfere with replication

A

1) little
2) directly
3) sterilize & disinfect
4) Ultraviolet
5) pyrimidine dimers

25
Q

What is ultraviolet rays used for?

A

airborne contaminants in hospital & operation rooms, school, waste water

25
Q

What is the normal UV range wavelength and which length is lethal?

A

normal: 100nm to 400nm
lethal: 240-280nm

26
Q

Describe chemical agents

A

-occur as liquid, gas, or solid
If solid may be dissolved in water (aqueous) and alcohol (tincture)

27
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29
Q
A