Chapter 14: Glycolysis Flashcards Preview

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Flashcards in Chapter 14: Glycolysis Deck (16)
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1
Q

what is glycolysis

A

central metabolic pathway

degradation of glucose to yield metabolic energy

2
Q

overall rxn

A

glucose + 6 O2 –> 6 CO2, + 6 H2O

3
Q

energy and molecules gained from glycolysis

A

2 NADH

2 ATP

2 pyruvate

4
Q

what is glycolysis coupled to?

A

ATP synthesis

5
Q

how many enzymes catalyze glycolysis?

A

10

6
Q

are all intermediates phosphorylated?

A

yes

negative charges on phosphate keep molecules inside cell b/c repel negative charges of plasma membrane

7
Q

prep phase

A

invest 2 ATP

8
Q

payoff phase

A

oxidation to pyruvate

formation of ATP and NADH

9
Q

5 types of rxns in glycolysis enzyme names

A

kinase

isomerase

aldolase

hydrogenase/dehydrogenase

dehydratase

10
Q

kinase

A

phosphate transfer from high E phosphate donating molecule to a substrate (or phosphate form substrate to ADP)

4 steps (1, 3, 7, 10)

11
Q

isomerase

A

intramolecular rearrangement

3 steps (2, 5, 8)

12
Q

aldolase

A

c-c bond cleavage

1 step (4)

13
Q

hydrogenase/dehydrogenase

A

oxidation reduction (also uses NADH and NAD+)

1 step (6) 2NAD + –> 2NADH + H+

14
Q

dehydratase

A

dehydration loss of water

1 step (9) lose 2 H2O b/c happens twice

15
Q

overall prep phase rxn

A

glucose + 2 ATP —> 2 glyceraldehyde 3-P + 2 ADP

costly (2 ATP)

phosphorylating the molecules keeps in cell

delta G is large and negative; essentially irreversible

16
Q

overall payoff phase rxn

A

(2) G-3-P + 2 NAD+ + 2Pi + 4ADP –> (2) pyruvate + 2 NADH + 4 ATP + 2 H2O

net gain of 2 ATP and 2 NADH

also gain 2 pyruvate