Chapter 2 Flashcards

1
Q

What does cell morphology mean?

A

The cell shape

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2
Q

What is the smallest microbe cell?

A

.2 micrometers-Mycoplasma sp.

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3
Q

What is the largest microbe cell?

A

700 micrometers-Epulopiscium fishelsoni

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4
Q

List two characteristics about Eupulopiscium fishelsoni.

A

It has thousands of copies of genome. It has unusual cell division.

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5
Q

Why should cells be small?

A

Larger surface-to-volume ratio, which gives a faster rate of nutrient exchange, faster growth, high population densities, and faster rates of evolution.

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6
Q

How small is too small for a cell? Why is this too small?

A

.15 micrometers. This is too small because they need space for proteins, nucleic acids, ribosomes, etc. This can create streamlined genomes, which results in missing genes.

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7
Q

What helps with the rigidity of the cytoplasmic membrane in eukaryotes? In bacteria?

A

Sterols; hopanoids

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8
Q

How is the membrane made in bacteria?

A

Ester linkages bond fatty acids to glcyerol

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9
Q

How are archaeal membranes different than other membranes?

A

Ether linkages bond hydrophobic side chains (isoprene) to glycerol, instead of binding fatty acids. Isoprene is composed of 5 hydrocarbons

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10
Q

What area do integral membrane proteins span?

A

Across the whole membrane

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11
Q

List three functions of the cytomplasmic membrane.

A
  1. Permability barrier
  2. Protein anchor
  3. Energy conservation
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12
Q

How does the cytoplasmic membrane serve as a permeability barrier?

A

It prevents leakage and functions as a gateway for transport of nutrients into, and wastes out of, the cell

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13
Q

How does the cytoplasmic membrane serve as a protein anchor?

A

It serves as a site of many proteins that participate in transports, bioenergetics, chemotaxis

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14
Q

How does the cytoplasmic membrane serve as energy conservation?

A

It serves as a site of generation and use of the proton motive force

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15
Q

Simple transport

A

Driven by the energy in the proton motive force

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16
Q

Group translocation

A

Chemical modification of the transported substance driven by phosphoenolpyruvate

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17
Q

ABC transporter

A

Periplasmic binding proteins are involved and energy comes from ATP

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18
Q

What system runs group translocation?

A

The phosphotransferase system

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19
Q

What happens during the use of the phosphotransferase system?

A

The substrate is chemically modified as it passes through the membrane. Translocates groups like fructose, glucose, and mannose.

20
Q

Describe ABC transport systems.

A

There are 200 systems of ATP-binding cassette proteins. This involves the uptake of sugars, amnio acids, sulfate, phosphate, and trace metals.

21
Q

Which bacterial cell has an outer membrane?

A

Gram-negative

22
Q

List 3 functions of cell walls.

A

They prevent osmotic lysing, they determine the shape, and they provide rigidity.

23
Q

What is peptidoglycan?

A

A polysaccharide composed mainly of N-acetylglucosamine and N-acetylmuramic acid

24
Q

What does the outer membrane contain?

A

Phospholipid, protein, and polysacchardides; AKA lipopolysaccharide layer (LPS)

25
List 4 characteristics of Lipid A.
Its an endotoxin, its toxic to animals, its in salmonella, shigella, and eschirchia, and causes gas, diarrhea, and vomiting.
26
Where is the periplasm located?
Between outer surface of cell membrane and inner surface of outer membrane
27
What does the periplasm contain?
Proteins, such as hydrolytic enzymes, binding enzymes, and chemoreceptors.
28
What are porins?
Channels for solutes. There are specific and nonspecific porins
29
How are porins composed?
3 identical subunits with 4 channels
30
What is the cell wall of some Archaea made of?
Pseudomurein
31
What is the S-layer in Archaea (and some bacteria)?
Its a paracrystalline surface layer of interlocking protein or glycoprotein molecules making up the outermost layer of wall.
32
What functions is the S-layer involved in?
Osmotic lysis, sieve, and retention of proteins
33
What are two polysaccharides layer types?
Capsules and slime layers
34
Capsules
organized, excludes particles
35
slime layer
disorganized, does not exclude particles, difficult to see
36
What functions do capsules/slime layers have?
Attachment of cells to surface (biofilms), pathogen evasion or immune system, and prevent desiccation.
37
Fimbriae
Filamentous structures of protein
38
Adhere to structures, such as what?
Neisseria, Salmonella, and Bordetalla
39
Pili is involved in what?
Conjugation and adhesion to host tissues, like Neisseria, and Streptococcus pyogenes
40
Type IV pili
Has twitching motility. Its colonization factors are vibrio and neisseria
41
Carbon and energy storage polymers
- Poly-beta-hydroxylalkonaotes | - Glycogen
42
Cell inclusions include _______ and ________.
polyphosphate and sulfur
43
Magnatosomes
Magnetite that responds to magnetic field, called magnetotaxis
44
Where are magnetosomes found?
In sediment bacteria, low O2 levels
45
Where are gas vesicles found?
In planktonic Bacteria and Archaea
46
What do gas vesicles do?
Confer buoyancy and allow cells to adjust to vertical position
47
Structure of gas vesicles
GvpA-vesicle shell GvpC-crosslinks GvpA -Gas-permeable -Watertight