CHAPTER 3 Flashcards
(45 cards)
What do you call to the microscopic study of the normal tissues of the body?
Histology
It is the microscopic study of tissues affected by disease.
Histopathology
The procedures adopted for the preparation of material for such studies are known as?
histologic or histopathologic techniques
It is the simplest, least invasive test and uses the smallest needle to simply remove cells from the area of abnormality.
Fine needle aspiration
It is a technique that removes not only cells, but also a small amount of the surrounding tissue.
core needle biopsy
Technique that takes out even more surrounding tissue. It takes out some of the abnormality, but not all.
If the lesion is found to be cancerous, further surgery may be needed to
incisional biopsy
Technique that generally removes the entire area in question.
excisional biopsy
A primary technique for obtaining
diagnostic full-thickness skin specimens.
Punch biopsy
It requires basic general
surgical and suture-tying skills
It is where small fragments of tissue are “shaved” from a surface (usually skin).
Shave biopsy
It is where tissue is scooped or spooned to remove tissue or growths from body cavity such as endometrium or cervical canal.
Curettings
Specimens are usually received in fixative (preservative) but sometimes they arrive fresh and must be immediately fixed.
True or False
True
Once tissues are removed from the body, their proteins and cells are digested and broken down by their own enzymes, independent of a bacterial action. What do you call this process?
autolysis
retarded by cold and accelerated at room temperature. It is more severe
Preserved tissues are usually examined when
there is an immediate need for evaluation.
True or False
False
Fresh tissues are usually examined when
there is an immediate need for e
What are some of the advantages of examining fresh tissue?
- Can be examined in the living state
- allow protoplasmic activities such as motion, mitosis, and phagocytosis to be observed.
Give the disadvantage of using fresh tissues
- tissues examined in the fresh state are not permanent
- liable to develop the changes that have usually been observed after death
What are the Methods of Fresh Tissue Examination?
- Teasing or Dissociation
- Squash Preparation (Crushing)
- Smear Preparation
- Touch Preparation (Impression Smear)
It is a process whereby a selected tissue specimen is immersed in isotonic salt solution such as normal saline or Ringer’s solution in a petri dish or watch glass, carefully dissected with a needle and separated by direct or zigzag spread using an applicator stick.
Teasing or Dissociation
Types of microscopy used for teasing or dissociation
Phase Contrast or Bright Field microscopy
Use of the phase contrast microscope greatly increases the structural detail of the cells examined in the living state, allowing movement and mitotic division to be observed.
True or False
True
It is a process whereby small pieces of tissue (not more than one mm. in diameter) are placed in a microscopic slide and forcibly compressed with another slide or with a cover glass.
Squash Preparation (Crushing)
Smear preparations can be made permanent by fixing them while still wet, staining them to demonstrate specific structures and inclusions, and mounting the cleared specimen beneath a cover glass with a suitable mounting medium.
True or False
True
It is a type of smear preparation where -With an applicator stick or a platinum loop, the material is rapidly and gently applied in a direct or zigzag line throughout the slide, attempting to obtain a relatively uniform distribution of secretion.
Streaking
A method of smear preparation where a selected portion of the material is transferred to a clean slide and gently spread into a moderately thick film by teasing the mucous strands apart with an applicator stick.
Spreading
It is a little more tedious than streaking, but has the advantage of mai
This is done by placing a drop of secretion or sedimentupon one slide and facing it to another clean slide. The material disperses evenly over the surface of the two slides.
Pull-Apart
