Chapter 3 Bacterial Genera

Question 1

What is the first stain of Gram-staining?

Answer 1

crystal violet

Question 2

What is the second stain of Gram-staining?

Answer 2

iodine

Question 3

What is the third stain of Gram-staining?

Answer 3

alcohol

Question 4

What is the fourth stain of Gram-staining?

Answer 4

safranin

Question 5

What is the pneumonic for bacteria that do not grain stain?

Answer 5

CRUM LACE

Question 6

Dark field microscopy is useful for detection of what bacteria?

Answer 6

spirochetes

Question 7

What two bacteria species grow on chocolate agar?

Answer 7

Neisseria and Haemophilus

Question 8

What bacteria grows on thayer martin?

Answer 8

Neisseria

Question 9

TCBS media is used to grow what species of bacteria?

Answer 9

Vibrio

Question 10

What two media are used to grow Bordatella pertussis?

Answer 10

Regan-Lowe and Bordet-Gengou

Question 11

What media is used to grow Legionella?

Answer 11

BCYE

Question 12

The Nagler test is used for what bacteria? What enzyme is being tested for?

Answer 12

Clostridium perfringens

lecithinase

Question 13

Catalse test is used to differentiate what two bacteria?

Answer 13

Staph (+) and Strep (-)

Question 14

Do gram-negative or gram-positive bacteria produce oxidase? What group of gram-negative bacteria do no produce oxidase?

Answer 14

Negative

Entero

Question 15

Nitrites in the urine are indicative of what bacteria? What enzyme accomplishes this reaction?

Answer 15

enterobactericiae

Nitrate reductase

Question 16

Chocolate agar is used to grow which two bacteria?

Answer 16

Neisseria and H. influenzae

Question 17

What UTI causing organism does not produce nitrate reductase?

Answer 17

S. saprophyticus

Question 18

What genus of bacteria is grown at 42C under microaerophilic conditions?

Answer 18

Camplyobacter

Question 19

What media can produce an oxygen gradient?

Answer 19

thioglycollate

Question 20

Is teichoic acid found in gram positive or gram negative bacteria?

Answer 20

gram positive

Question 21

Are streptococci catalase negative or positive?

Answer 21

negative

Question 22

Is S. epidermidis coagulase positive or negative?

Answer 22

negative

Question 23

Is S. saprophytiucus coagulase positive or negative?

Answer 23

negative

Question 24

Is S. pneumoniae bile-soluble or bile-insoluble?

Answer 24

bile soluble

Question 25

What is the most important bug in GAS?

Answer 25

Strep pyogenes

Question 26

What is the most important bug in GBS?

Answer 26

Strep. agalactiae