Chapter 6 Flashcards

microbial growth and nutrition (42 cards)

1
Q

What are the essential nutrients of a bacterial cell?

A

Carbon, hydrogen, nitrogen, oxygen, phosphorus, sulfur (CHNOPS)

These nutrients are crucial for various cellular functions and structures.

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2
Q

Differentiate between macronutrients and micronutrients.

A

Macronutrients are required in large quantities and are crucial for cell structure and metabolism, while micronutrients are present in smaller amounts and are involved in enzyme function and protein structure

Examples of macronutrients include CHNOPS, while micronutrients include trace elements like manganese, zinc, and nickel.

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3
Q

List and define the four terms that describe an organism’s sources of carbon and energy.

A
  • Heterotroph: obtains carbon in organic form
  • Autotroph: uses inorganic CO2 as carbon source
  • Phototroph: gets energy from sunlight
  • Chemotroph: gets energy from chemical compounds

These terms categorize organisms based on their nutritional requirements.

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4
Q

What is a saprobe?

A

An organism that derives nutrients from decaying organic matter

Saprobes are essential for recycling nutrients in ecosystems.

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5
Q

What is a parasite?

A

An organism that derives nutrients from the cells or tissues of a living host

This can lead to damage or death of the host.

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6
Q

True or False: The terms saprobe and parasite can oversimplify the nutritional strategies of some organisms.

A

True

These terms may not encompass the complexity of certain organisms’ lifestyles.

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7
Q

Compare diffusion and osmosis.

A

Diffusion is the movement of molecules from high to low concentration; osmosis specifically refers to the movement of water across a selectively permeable membrane.

Both processes are passive transport mechanisms.

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8
Q

Identify the effects of isotonic, hypotonic, and hypertonic conditions on a cell.

A
  • Isotonic: no net movement of water
  • Hypotonic: water enters the cell, causing it to swell
  • Hypertonic: water leaves the cell, leading to plasmolysis

Understanding these effects is crucial for cell biology and microbiology.

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9
Q

Name two types of passive transport.

A
  • Simple diffusion
  • Facilitated diffusion

Both transport mechanisms do not require energy.

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10
Q

What is one type of active transport?

A

Carrier-mediated active transport

This process requires energy to move substances against their concentration gradient.

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11
Q

What is the optimum temperature range for mesophiles?

A

Between 20°C and 40°C

Most medically significant microorganisms fall into this category.

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12
Q

Define psychrophiles.

A

Microbes that have an optimum temperature below 15°C and can grow at 0°C

They are typically found in cold environments like polar ice and deep oceans.

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13
Q

What organisms are classified as thermophiles?

A

Microbes that grow optimally at temperatures greater than 45°C

They are often found in environments associated with volcanic activity.

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14
Q

What is the definition of obligate aerobes?

A

Organisms that cannot grow without oxygen

Examples include many fungi and certain bacteria like Bacillus species.

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15
Q

Fill in the blank: An organism that does not utilize oxygen but can survive in its presence is called an _______.

A

Aerotolerant anaerobe

These organisms do not use oxygen in metabolism but can tolerate it.

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16
Q

What are the three types of associations microbes can have with their hosts?

A
  • Mutualism
  • Commensalism
  • Parasitism

Each association has different implications for the host and the microbe.

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17
Q

What are anaerobes?

A

Microbes that lack the metabolic enzyme systems for using oxygen in respiration.

18
Q

Define aerotolerant anaerobes.

A

Organisms that do not utilize oxygen but can survive and grow to a limited extent in its presence.

19
Q

What are facultative anaerobes?

A

Microbes that do not require oxygen for metabolism but can use it when it is present.

20
Q

Give examples of facultative anaerobes.

A
  • Many gram-negative intestinal bacteria
  • Staphylococci
21
Q

What are the three categories of microbes based on oxygen usage?

A
  • Those that use oxygen and detoxify it
  • Those that can neither use oxygen nor detoxify it
  • Those that do not use oxygen but can detoxify it
22
Q

Which atmospheric gas has the greatest impact on microbial growth?

23
Q

What is the pH scale?

A

A series of numbers ranging from 0 to 14 that expresses the degree of acidity or alkalinity of a solution.

24
Q

What is the pH of pure water?

25
What are osmophiles?
Microbes that live in habitats with high solute concentration.
26
Define halophiles.
Microbes that prefer high concentrations of salt.
27
What are obligate halophiles?
Organisms that grow optimally at solutions of 25% NaCl but require at least 9% NaCl.
28
What are facultative halophiles?
Microbes that are remarkably resistant to salt, even though they do not normally reside in high salt environments.
29
What are barophiles?
Bacteria that exist under pressures ranging from a few times to over 1,000 times the pressure of the atmosphere.
30
Define symbiosis.
A general term to denote a situation in which two organisms live together in a close partnership.
31
What is mutualism?
A type of symbiosis where organisms live in an obligatory but mutually beneficial relationship.
32
What is commensalism?
A relationship where one partner receives benefits while the other is neither harmed nor benefited.
33
What is parasitism?
A relationship where the host organism provides the parasitic microbe with nutrients and a habitat, and the host suffers from the relationship.
34
What is binary fission?
The process by which one cell becomes two.
35
What is the average generation time for most bacteria?
30 to 60 minutes.
36
What is the generation time for Mycobacterium leprae?
10 to 30 days.
37
What are the four phases of a bacterial growth curve?
* Lag phase * Exponential phase * Stationary phase * Death phase
38
What happens during the log phase of the bacterial growth curve?
The bacteria undergo rapid exponential growth, doubling at a constant rate due to optimal conditions.
39
What is turbidity in relation to microbial growth?
A clear nutrient solution becomes turbid, or cloudy, as microbes grow in it.
40
What is a Coulter counter?
An electronic device that scans a fluid as it passes through a tiny pipette to count cells.
41
True or False: Biofilms are composed solely of a single species of microorganism.
False
42
What is one benefit of biofilms for microorganisms?
They allow microorganisms to share nutrients and exchange genetic material.