Chapter 7 - Enzyme kinetics

Question 1

What does competitive inhibitor affect? How does it change?

Answer 1

Km but not Vmax

Km increases

Question 2

competitive inhibitor

Answer 2

the compound is similar to substrate and compete for the sams site

Question 3

In presence of competitive inhibitor, what could be done to overcome effect of this inhibitor?

Answer 3

increase the substrate concentrations

Question 4

uncompetitve inhibitor

Answer 4

inhibitor binds to site other than the substrate binding pocket only when the substrate is present

Question 5

noncompetitve inhibitor

Answer 5

the inhibitor bind to other site than substrate binding pocket whether substrate is present or not

Question 6

what does uncompetitive inhibitor affect?

Answer 6

affect both Vmax and Km

Question 7

What does noncompetitve inhibitor affect( in pure and mixed inhibition)

Answer 7

Vmax decrease but Km stays the same (in pure)

Km increases and Vmax decreases (in mixed)

Question 8

What does noncompetitve inhibitor can bind?

“pure” noncompetitve inhibitor

Answer 8

free E or ES complex

if binds free E and ES same

Question 9

What does uncompetitive inhibitor bind? competitive inhibitor?

Answer 9

only ES complex

only E (enzyme acive site)

Question 10

What does K(i) measure? Its equation?

Answer 10

measure affinity btw enzyme and inhibitor

[E][I]/ [EI]

Question 11

K(m) increases mean that??

Answer 11

the substrate concentration also increase to reach the same vmax/2