Chapter 7 - Enzyme kinetics Flashcards by luan nguyen | Brainscape

Flashcards in Chapter 7 - Enzyme kinetics Deck (24)

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1

What does competitive inhibitor affect? How does it change?

Km but not Vmax

Km increases

2

competitive inhibitor

the compound is similar to substrate and compete for the sams site

3

In presence of competitive inhibitor, what could be done to overcome effect of this inhibitor?

increase the substrate concentrations

4

uncompetitve inhibitor

inhibitor binds to site other than the substrate binding pocket only when the substrate is present

5

noncompetitve inhibitor

the inhibitor bind to other site than substrate binding pocket whether substrate is present or not

6

what does uncompetitive inhibitor affect?

affect both Vmax and Km

7

What does noncompetitve inhibitor affect( in pure and mixed inhibition)

Vmax decrease but Km stays the same (in pure)

Km increases and Vmax decreases (in mixed)

8

What does noncompetitve inhibitor can bind?

"pure" noncompetitve inhibitor

free E or ES complex

if binds free E and ES same

9

What does uncompetitive inhibitor bind? competitive inhibitor?

only ES complex

only E (enzyme acive site)

10

What does K(i) measure? Its equation?

measure affinity btw enzyme and inhibitor

[E][I]/ [EI]

11

K(m) increases mean that??

the substrate concentration also increase to reach the same vmax/2

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