What does competitive inhibitor affect? How does it change?
Km but not Vmax
Km increases
competitive inhibitor
the compound is similar to substrate and compete for the sams site
In presence of competitive inhibitor, what could be done to overcome effect of this inhibitor?
increase the substrate concentrations
uncompetitve inhibitor
inhibitor binds to site other than the substrate binding pocket only when the substrate is present
noncompetitve inhibitor
the inhibitor bind to other site than substrate binding pocket whether substrate is present or not
what does uncompetitive inhibitor affect?
affect both Vmax and Km
What does noncompetitve inhibitor affect( in pure and mixed inhibition)
Vmax decrease but Km stays the same (in pure)
Km increases and Vmax decreases (in mixed)
What does noncompetitve inhibitor can bind?
“pure” noncompetitve inhibitor
free E or ES complex
if binds free E and ES same
What does uncompetitive inhibitor bind? competitive inhibitor?
only ES complex
only E (enzyme acive site)
What does K(i) measure? Its equation?
measure affinity btw enzyme and inhibitor
[E][I]/ [EI]
K(m) increases mean that??
the substrate concentration also increase to reach the same vmax/2