Cholexterol Determination

Question 1

Cholesterol Esterase (CE) ________ esters to free cholesterol and fatty acids

Answer 1

hydrolyzes

Question 2

wavelength?

Answer 2

500 nm

Question 3

Reagent and Standard are stable stored ________ until expiration date on label

Answer 3

2-8 degrees celcius

Question 4

what temperature must a medtech bring the reagent and standard before use

Answer 4

Room Temperature

Question 5

Blood should be collected following a _________ specimen

Answer 5

12 hour fast

Question 6

What should be avoided in collecting sample

Answer 6

hemolysis

Question 7

cholesterol standard

Answer 7

200mg/dL

Question 8

What color is the intensity proportional to total cholesterol concentration?

Answer 8

Red color or O-Quinoneimine dye

Question 9

Incubate all cuvettes at ______ for _____ minutes

Answer 9

37 degree celcius, 5 minutes

Question 10

How many mL/uL

Standard- Standard (s)

Answer 10

0.01 mL (10uL)

Question 11

Reagent: RB, S, U

Answer 11

1.0mL

Question 12

Sample to Sample (U)

Answer 12

0.01 mL (10uL)

Question 13

Read S and U vs RB at ____ nm within ___ minutes

Answer 13

500 nm, 60 minutes

Question 14

Reference Range: Adolescent

Answer 14

120-210 mg/dL

Question 15

Reference Range: Adult

Answer 15

140-310 mg/dL

Question 16

Reference Range: Desirable for Adult

Answer 16

140-200 mg/dL

Question 17

Reference Range: Newborn

Answer 17

53-135 mg/dL

Question 18

Reference Range: Infant

Answer 18

70-175 mg/dL

Question 19

Reference Range: Child

Answer 19

120-200 mg/dL

Question 20

_______________ with absorption maxima at 500 nm is produced when phenol is oxidatively coupled with ______________ in the presence of ______________ with ___________

Answer 20

Quinoneimine Chromogen
4-aminophenazone
Peroxidase
Hydrogen Peroxide

Question 21

How many months can extend the stabilities at -20C for Total Cholesterol?

Answer 21

3 months

Question 22

How many days can extend the stabilities at -20C for HDL Cholesterol?

Answer 22

7-14 days

Question 23

Interfering substance: Anticoagulants such as ______________ and _____________ will result in false low values

Answer 23

sodium flouride and oxalate

Question 24

Cholesterol Reagent Components

Answer 24

4-aminophenazone
Phenol
Peroxidase
Cholesterol Esterase
Cholesterol Oxidase
Buffers and Stabilizers

Question 25

The free cholesterol so produced plus the performed cholesterol is the __________ in the presence of _____________________ to__________________ and ___________________

Answer 25

oxidized cholesterol oxidase cholest-4-en-3-en-one hydrogen peroxide

Question 26

Cholesterol Determination Method

Answer 26

Cholesterol Oxidase Method

Question 27

Primary Reaction

Question 28

Secondary Reaction

Question 29

Tertiary Reaction

Question 30

2 forms of Cholesterol: measure ng method

Answer 30

free cholesterol

Question 31

2 forms of Cholesterol: Serum

Answer 31

Cholesterol esters

Question 32

Reaction: end product ng 2nd reaction

Answer 32

cholest-4-en-3-en-one and Hydrogen Peroxide

Question 33

Reaction: color developing

Answer 33

4-aminophenazone+ Phenol

Question 34

what enzyme are use in secondary reaction?

Answer 34

cholesterol oxidase

Question 35

what enzyme are used in primary reaction?

Answer 35

cholesterol esterase

Question 36

what enzyme are used in tertiary reaction?

Answer 36

peroxidase

Question 37

The test is not influenced by ____________ values up to _____________ mg/dL or by ____________ levels up to ______ mg/dL

Answer 37

hemoglobin 200mg/dL bilirubin 10 mg/dL

Question 38

Pang zero, calibrate sa reading

Answer 38

Reagent Blank